Food Restriction Induces Changes in Ovarian Folliculogenesis, Cell Proliferation, Apoptosis, and Production of Regulatory Peptides in Rabbits

The aim of this study is to examine the influence of food restriction on rabbit ovarian functions. A total of eight females were fed ad libitum (NF), while eight females were subjected to 50% food restriction (RF). One month later, all females were euthanized. Weights and lengths of ovaries and uter...

Descripción completa

Detalles Bibliográficos
Autores: Hadjadj, Imane, Fabova, Zuzana, Garcia Pardo, Maria Luz, Agea, Iván, Loncová, Barbora, Morovic, Martin, Makovicky, Peter, Argente, María José, Sirotkin, Alexander V.
Tipo de recurso: artículo
Fecha de publicación:2025
País:España
Institución:Universidad Miguel Hernández de Elche
Repositorio:REDIUMH. Depósito Digital de la UMH
OAI Identifier:oai:dspace.umh.es:11000/36551
Acceso en línea:https://hdl.handle.net/11000/36551
Access Level:acceso abierto
Palabra clave:food restriction
apoptosis
hormone
ovarian follicle
rabbit
id ES_db4cbbdd9b5f8cd3650a74e2b7031a9a
oai_identifier_str oai:dspace.umh.es:11000/36551
network_acronym_str ES
network_name_str España
repository_id_str
spelling Food Restriction Induces Changes in Ovarian Folliculogenesis, Cell Proliferation, Apoptosis, and Production of Regulatory Peptides in RabbitsHadjadj, ImaneFabova, ZuzanaGarcia Pardo, Maria LuzAgea, IvánLoncová, BarboraMorovic, MartinMakovicky, PeterArgente, María JoséSirotkin, Alexander V.food restrictionapoptosishormoneovarian folliclerabbitThe aim of this study is to examine the influence of food restriction on rabbit ovarian functions. A total of eight females were fed ad libitum (NF), while eight females were subjected to 50% food restriction (RF). One month later, all females were euthanized. Weights and lengths of ovaries and uterine horns were measured. Representative parts of the ovaries were subjected to histomorphometry analysis of folliculogenesis. Granulosa cells were isolated and cell viability, proliferation (accumulation of PCNA, cyclin B1, and BrdU-positive cells), apoptosis (accumulation of bax, caspase 3, and DNA fragmentation) were evaluated. Granulosa cells were subjected to proteomic analysis by using the nano HPLC-Chip-MS/MS method. Estradiol and progesterone release by ovarian and granulosa cells was assessed by ELISA. Ovarian and uterine horn weights were lower in RF than NF. The diameter of follicles and oocytes and the thickness of the theca and granulosa cells were higher in RF than NF. RF showed a lower percentage of cells containing bax and caspase 3, occurrence of DNA fragmented cells, and estradiol and progesterone. RF had higher incorporation of BrdU, a higher proportion of cells containing PCNA and cyclin B1, and a lower percentage of viable cells. RF produced more specific proteins than NF, including peptides involved in cell differentiation, proliferation/division, mitotic cell cycle, and GTP-ase activity. In conclusion, food restriction can activate reproduction by (1) selection of the growing primordial follicles, (2) better transformation of secondary to preovulatory follicles, (3) increasing growth of oocytes, (4) increasing proliferation and decreasing apoptosis in granulosa cells, (5) changes in ovarian secretory activity, and (6) changes in the number of peptides.MDPIDepartamentos de la UMH::Tecnología Agroalimentaria202520252025info:eu-repo/semantics/articleapplication/pdf19application/pdfhttps://hdl.handle.net/11000/36551reponame:REDIUMH. Depósito Digital de la UMHinstname:Universidad Miguel Hernández de ElcheIngléshttps://doi.org/10.3390/ani15091282info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0/oai:dspace.umh.es:11000/365512026-05-27T13:36:21Z
dc.title.none.fl_str_mv Food Restriction Induces Changes in Ovarian Folliculogenesis, Cell Proliferation, Apoptosis, and Production of Regulatory Peptides in Rabbits
title Food Restriction Induces Changes in Ovarian Folliculogenesis, Cell Proliferation, Apoptosis, and Production of Regulatory Peptides in Rabbits
spellingShingle Food Restriction Induces Changes in Ovarian Folliculogenesis, Cell Proliferation, Apoptosis, and Production of Regulatory Peptides in Rabbits
Hadjadj, Imane
food restriction
apoptosis
hormone
ovarian follicle
rabbit
title_short Food Restriction Induces Changes in Ovarian Folliculogenesis, Cell Proliferation, Apoptosis, and Production of Regulatory Peptides in Rabbits
title_full Food Restriction Induces Changes in Ovarian Folliculogenesis, Cell Proliferation, Apoptosis, and Production of Regulatory Peptides in Rabbits
title_fullStr Food Restriction Induces Changes in Ovarian Folliculogenesis, Cell Proliferation, Apoptosis, and Production of Regulatory Peptides in Rabbits
title_full_unstemmed Food Restriction Induces Changes in Ovarian Folliculogenesis, Cell Proliferation, Apoptosis, and Production of Regulatory Peptides in Rabbits
title_sort Food Restriction Induces Changes in Ovarian Folliculogenesis, Cell Proliferation, Apoptosis, and Production of Regulatory Peptides in Rabbits
dc.creator.none.fl_str_mv Hadjadj, Imane
Fabova, Zuzana
Garcia Pardo, Maria Luz
Agea, Iván
Loncová, Barbora
Morovic, Martin
Makovicky, Peter
Argente, María José
Sirotkin, Alexander V.
author Hadjadj, Imane
author_facet Hadjadj, Imane
Fabova, Zuzana
Garcia Pardo, Maria Luz
Agea, Iván
Loncová, Barbora
Morovic, Martin
Makovicky, Peter
Argente, María José
Sirotkin, Alexander V.
author_role author
author2 Fabova, Zuzana
Garcia Pardo, Maria Luz
Agea, Iván
Loncová, Barbora
Morovic, Martin
Makovicky, Peter
Argente, María José
Sirotkin, Alexander V.
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Departamentos de la UMH::Tecnología Agroalimentaria
dc.subject.none.fl_str_mv food restriction
apoptosis
hormone
ovarian follicle
rabbit
topic food restriction
apoptosis
hormone
ovarian follicle
rabbit
description The aim of this study is to examine the influence of food restriction on rabbit ovarian functions. A total of eight females were fed ad libitum (NF), while eight females were subjected to 50% food restriction (RF). One month later, all females were euthanized. Weights and lengths of ovaries and uterine horns were measured. Representative parts of the ovaries were subjected to histomorphometry analysis of folliculogenesis. Granulosa cells were isolated and cell viability, proliferation (accumulation of PCNA, cyclin B1, and BrdU-positive cells), apoptosis (accumulation of bax, caspase 3, and DNA fragmentation) were evaluated. Granulosa cells were subjected to proteomic analysis by using the nano HPLC-Chip-MS/MS method. Estradiol and progesterone release by ovarian and granulosa cells was assessed by ELISA. Ovarian and uterine horn weights were lower in RF than NF. The diameter of follicles and oocytes and the thickness of the theca and granulosa cells were higher in RF than NF. RF showed a lower percentage of cells containing bax and caspase 3, occurrence of DNA fragmented cells, and estradiol and progesterone. RF had higher incorporation of BrdU, a higher proportion of cells containing PCNA and cyclin B1, and a lower percentage of viable cells. RF produced more specific proteins than NF, including peptides involved in cell differentiation, proliferation/division, mitotic cell cycle, and GTP-ase activity. In conclusion, food restriction can activate reproduction by (1) selection of the growing primordial follicles, (2) better transformation of secondary to preovulatory follicles, (3) increasing growth of oocytes, (4) increasing proliferation and decreasing apoptosis in granulosa cells, (5) changes in ovarian secretory activity, and (6) changes in the number of peptides.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/11000/36551
url https://hdl.handle.net/11000/36551
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.3390/ani15091282
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.format.none.fl_str_mv application/pdf
19
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:REDIUMH. Depósito Digital de la UMH
instname:Universidad Miguel Hernández de Elche
instname_str Universidad Miguel Hernández de Elche
reponame_str REDIUMH. Depósito Digital de la UMH
collection REDIUMH. Depósito Digital de la UMH
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869421662033674240
score 15,812429

Similares en LA Referencia