Efficient production of isomelezitose by a glucosyltransferase activity in Metschnikowia reukaufii cell extracts

[EN] Metschnikowia reukaufii is a widespread yeast able to grow in the plants’ floral nectaries, an environment of extreme conditions with sucrose concentrations exceeding 400 g l−1, which led us into the search for enzymatic activities involved in this sugar use/transformation. New oligosaccharides...

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Autores: García-González, Martín, Plou Gasca, Francisco José, Cervantes, Fadia V., Remacha, Miguel, Poveda, Ana, Jiménez-Barbero, Jesús, Fernández Lobato, María
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/205996
Acceso en línea:http://hdl.handle.net/10261/205996
Access Level:acceso abierto
Palabra clave:Isomelezitose
Glucosyltransferase activity
Metschnikowia reukaufii
Cell extracts
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spelling Efficient production of isomelezitose by a glucosyltransferase activity in Metschnikowia reukaufii cell extractsGarcía-González, MartínPlou Gasca, Francisco JoséCervantes, Fadia V.Remacha, MiguelPoveda, AnaJiménez-Barbero, JesúsFernández Lobato, MaríaIsomelezitoseGlucosyltransferase activityMetschnikowia reukaufiiCell extracts[EN] Metschnikowia reukaufii is a widespread yeast able to grow in the plants’ floral nectaries, an environment of extreme conditions with sucrose concentrations exceeding 400 g l−1, which led us into the search for enzymatic activities involved in this sugar use/transformation. New oligosaccharides were produced by transglucosylation processes employing M. reukaufii cell extracts in overload‐sucrose reactions. These products were purified and structurally characterized by MS‐ESI and NMR techniques. The reaction mixture included new sugars showing a great variety of glycosidic bonds including α‐(1→1), α‐(1→3) and α‐(1→6) linkages. The main product synthesized was the trisaccharide isomelezitose, whose maximum concentration reached 81 g l−1, the highest amount reported for any unmodified enzyme or microbial extract. In addition, 51 g l−1 of the disaccharide trehalulose was also produced. Both sugars show potential nutraceutical and prebiotic properties. Interestingly, the sugar mixture obtained in the biosynthetic reactions also contained oligosaccharides such as esculose, a rare trisaccharide with no previous NMR structure elucidation, as well as erlose, melezitose and theanderose. All the sugars produced are naturally found in honey. These compounds are of biotechnological interest due to their potential food, cosmeceutical and pharmaceutical applications.This work was supported by the Projects BIO2016‐76601‐C3‐2‐R/‐1‐R from the Spanish Ministry of Economy and Competitiveness and Fundación Ramón Areces (XIX Call of Research Grants in Life and Material Sciences). Martin Garcia‐Gonzalez fellowship FPU16/02925 was supported by the Spanish Ministry of Education, Culture and Sport. We thank Fundación Ramón Areces for an institutional grant to the Center of Molecular Biology Severo Ochoa. We also thank Mrs. Asunción Martín‐Redondo for the technical support.John Wiley & SonsMinisterio de Economía y Competitividad (España)Fundación Ramón ArecesMinisterio de Educación, Cultura y Deporte (España)Plou Gasca, Francisco José [0000-0003-0831-893X]Fernández-Lobato, María [0000-0003-0778-7443]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2020202020192020info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/205996reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2016-76601-C3-2-Rhttp://dx.doi.org/10.1111/1751-7915.13490Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2059962026-05-22T06:33:51Z
dc.title.none.fl_str_mv Efficient production of isomelezitose by a glucosyltransferase activity in Metschnikowia reukaufii cell extracts
title Efficient production of isomelezitose by a glucosyltransferase activity in Metschnikowia reukaufii cell extracts
spellingShingle Efficient production of isomelezitose by a glucosyltransferase activity in Metschnikowia reukaufii cell extracts
García-González, Martín
Isomelezitose
Glucosyltransferase activity
Metschnikowia reukaufii
Cell extracts
title_short Efficient production of isomelezitose by a glucosyltransferase activity in Metschnikowia reukaufii cell extracts
title_full Efficient production of isomelezitose by a glucosyltransferase activity in Metschnikowia reukaufii cell extracts
title_fullStr Efficient production of isomelezitose by a glucosyltransferase activity in Metschnikowia reukaufii cell extracts
title_full_unstemmed Efficient production of isomelezitose by a glucosyltransferase activity in Metschnikowia reukaufii cell extracts
title_sort Efficient production of isomelezitose by a glucosyltransferase activity in Metschnikowia reukaufii cell extracts
dc.creator.none.fl_str_mv García-González, Martín
Plou Gasca, Francisco José
Cervantes, Fadia V.
Remacha, Miguel
Poveda, Ana
Jiménez-Barbero, Jesús
Fernández Lobato, María
author García-González, Martín
author_facet García-González, Martín
Plou Gasca, Francisco José
Cervantes, Fadia V.
Remacha, Miguel
Poveda, Ana
Jiménez-Barbero, Jesús
Fernández Lobato, María
author_role author
author2 Plou Gasca, Francisco José
Cervantes, Fadia V.
Remacha, Miguel
Poveda, Ana
Jiménez-Barbero, Jesús
Fernández Lobato, María
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Fundación Ramón Areces
Ministerio de Educación, Cultura y Deporte (España)
Plou Gasca, Francisco José [0000-0003-0831-893X]
Fernández-Lobato, María [0000-0003-0778-7443]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Isomelezitose
Glucosyltransferase activity
Metschnikowia reukaufii
Cell extracts
topic Isomelezitose
Glucosyltransferase activity
Metschnikowia reukaufii
Cell extracts
description [EN] Metschnikowia reukaufii is a widespread yeast able to grow in the plants’ floral nectaries, an environment of extreme conditions with sucrose concentrations exceeding 400 g l−1, which led us into the search for enzymatic activities involved in this sugar use/transformation. New oligosaccharides were produced by transglucosylation processes employing M. reukaufii cell extracts in overload‐sucrose reactions. These products were purified and structurally characterized by MS‐ESI and NMR techniques. The reaction mixture included new sugars showing a great variety of glycosidic bonds including α‐(1→1), α‐(1→3) and α‐(1→6) linkages. The main product synthesized was the trisaccharide isomelezitose, whose maximum concentration reached 81 g l−1, the highest amount reported for any unmodified enzyme or microbial extract. In addition, 51 g l−1 of the disaccharide trehalulose was also produced. Both sugars show potential nutraceutical and prebiotic properties. Interestingly, the sugar mixture obtained in the biosynthetic reactions also contained oligosaccharides such as esculose, a rare trisaccharide with no previous NMR structure elucidation, as well as erlose, melezitose and theanderose. All the sugars produced are naturally found in honey. These compounds are of biotechnological interest due to their potential food, cosmeceutical and pharmaceutical applications.
publishDate 2019
dc.date.none.fl_str_mv 2019
2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/205996
url http://hdl.handle.net/10261/205996
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2016-76601-C3-2-R
http://dx.doi.org/10.1111/1751-7915.13490
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv John Wiley & Sons
publisher.none.fl_str_mv John Wiley & Sons
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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