Evaluation of three enzyme-linked immunosorbent assays for sarcoptic mange diagnosis and assessment in the Iberian ibex, Capra pyrenaica
Background: Sarcoptic mange is a contagious skin disease caused by the mite Sarcoptes scabiei, affecting different mammalian species worldwide including the Iberian ibex (Capra pyrenaica), in which mortalities over 90 % of the population have been reported. No efficient diagnostic methods are availa...
| Autores: | , , , , , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2016 |
| País: | España |
| Institución: | Universitat Autònoma de Barcelona |
| Repositorio: | Dipòsit Digital de Documents de la UAB |
| Idioma: | inglés |
| OAI Identifier: | oai:ddd.uab.cat:203422 |
| Acceso en línea: | https://ddd.uab.cat/record/203422 https://dx.doi.org/urn:doi:10.1186/s13071-016-1843-4 |
| Access Level: | acceso abierto |
| Palabra clave: | Veterinària Capra pyrenaica Diagnostic ELISA Sarcoptes scabiei Serum |
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Evaluation of three enzyme-linked immunosorbent assays for sarcoptic mange diagnosis and assessment in the Iberian ibex, Capra pyrenaicaRáez-Bravo, Arián|||0000-0002-3190-1659Granados Torres, José Enrique|||0000-0002-9787-9896Serrano Ferron, Emmanuel|||0000-0002-9799-9804Dellamaria, DeboraCasais, Rosa|||0000-0001-8349-5878Rossi, Luca|||0000-0001-6829-5229Puigdemont Rodriguez, Anna|||0000-0001-5219-7552Cano-Manuel, Francisco JavierFandos, Paulino|||0000-0002-9607-8931Pérez Jiménez, Jesús María|||0000-0001-9159-0365Espinosa, José|||0000-0002-9036-1402Soriguer, Ramón C|||0000-0002-9165-7766Citterio, CarloLópez Olvera, Jorge R.|||0000-0002-2999-3451VeterinàriaCapra pyrenaicaDiagnosticELISASarcoptes scabieiSerumBackground: Sarcoptic mange is a contagious skin disease caused by the mite Sarcoptes scabiei, affecting different mammalian species worldwide including the Iberian ibex (Capra pyrenaica), in which mortalities over 90 % of the population have been reported. No efficient diagnostic methods are available for this disease, particularly when there are low mite numbers and mild or no clinical signs. In this study, three enzyme-linked immunosorbent assays (ELISA) developed for dog (ELISA A), Cantabrian chamois (Rupicapra pyrenaica parva) (ELISA B) and Alpine chamois (Rupicapra rupicapra) (ELISA C), were evaluated to detect specific antibodies (IgG) to sarcoptic mange in Iberian ibex sera. Methods: Serum samples from 131 Iberian ibexes (86 healthy and 45 scabietic) were collected from 2005 to 2012 in the Sierra Nevada Natural and National Parks (southern Spain). Based on visual inspection, ibexes were classified into one of three categories, namely healthy (without scabietic compatible lesions), mildly affected (skin lesions over less than 50 % of the body surface) and severely affected (skin lesions over more than 50 % of the body surface). The optimal cut-off point, specificity, sensitivity and the area under the curve (AUC) were calculated, and the agreement between tests was determined. Moreover, differences in the optical density (OD) related to scabies severity have been evaluated for the best test. Results: ELISA C showed better performance than the two other tests, reaching higher values of sensitivity (93.0 %) and specificity (93.5 %) against the visual estimation of the percentage of affected skin, chosen as the gold standard. Significantly higher concentrations of specific antibodies were observed with this test in the mildly and severely infested ibexes than in healthy ones. Conclusions: Our results revealed that ELISA C was an optimal test to diagnose sarcoptic mange in the Iberian ibex. Further studies characterizing immune response during the course of the disease, including spontaneous or drug induced recovery, should follow in order to better understand sarcoptic mange in Iberian ibex populations. 22016-01-0120162016-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/203422https://dx.doi.org/urn:doi:10.1186/s13071-016-1843-4reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengMinisterio de Economía y Competitividad https://doi.org/10.13039/501100003329 CGL2012-40043-C02-02Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 CGL2016-80543-Popen accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2034222026-06-06T12:50:31Z |
| dc.title.none.fl_str_mv |
Evaluation of three enzyme-linked immunosorbent assays for sarcoptic mange diagnosis and assessment in the Iberian ibex, Capra pyrenaica |
| title |
Evaluation of three enzyme-linked immunosorbent assays for sarcoptic mange diagnosis and assessment in the Iberian ibex, Capra pyrenaica |
| spellingShingle |
Evaluation of three enzyme-linked immunosorbent assays for sarcoptic mange diagnosis and assessment in the Iberian ibex, Capra pyrenaica Ráez-Bravo, Arián|||0000-0002-3190-1659 Veterinària Capra pyrenaica Diagnostic ELISA Sarcoptes scabiei Serum |
| title_short |
Evaluation of three enzyme-linked immunosorbent assays for sarcoptic mange diagnosis and assessment in the Iberian ibex, Capra pyrenaica |
| title_full |
Evaluation of three enzyme-linked immunosorbent assays for sarcoptic mange diagnosis and assessment in the Iberian ibex, Capra pyrenaica |
| title_fullStr |
Evaluation of three enzyme-linked immunosorbent assays for sarcoptic mange diagnosis and assessment in the Iberian ibex, Capra pyrenaica |
| title_full_unstemmed |
Evaluation of three enzyme-linked immunosorbent assays for sarcoptic mange diagnosis and assessment in the Iberian ibex, Capra pyrenaica |
| title_sort |
Evaluation of three enzyme-linked immunosorbent assays for sarcoptic mange diagnosis and assessment in the Iberian ibex, Capra pyrenaica |
| dc.creator.none.fl_str_mv |
Ráez-Bravo, Arián|||0000-0002-3190-1659 Granados Torres, José Enrique|||0000-0002-9787-9896 Serrano Ferron, Emmanuel|||0000-0002-9799-9804 Dellamaria, Debora Casais, Rosa|||0000-0001-8349-5878 Rossi, Luca|||0000-0001-6829-5229 Puigdemont Rodriguez, Anna|||0000-0001-5219-7552 Cano-Manuel, Francisco Javier Fandos, Paulino|||0000-0002-9607-8931 Pérez Jiménez, Jesús María|||0000-0001-9159-0365 Espinosa, José|||0000-0002-9036-1402 Soriguer, Ramón C|||0000-0002-9165-7766 Citterio, Carlo López Olvera, Jorge R.|||0000-0002-2999-3451 |
| author |
Ráez-Bravo, Arián|||0000-0002-3190-1659 |
| author_facet |
Ráez-Bravo, Arián|||0000-0002-3190-1659 Granados Torres, José Enrique|||0000-0002-9787-9896 Serrano Ferron, Emmanuel|||0000-0002-9799-9804 Dellamaria, Debora Casais, Rosa|||0000-0001-8349-5878 Rossi, Luca|||0000-0001-6829-5229 Puigdemont Rodriguez, Anna|||0000-0001-5219-7552 Cano-Manuel, Francisco Javier Fandos, Paulino|||0000-0002-9607-8931 Pérez Jiménez, Jesús María|||0000-0001-9159-0365 Espinosa, José|||0000-0002-9036-1402 Soriguer, Ramón C|||0000-0002-9165-7766 Citterio, Carlo López Olvera, Jorge R.|||0000-0002-2999-3451 |
| author_role |
author |
| author2 |
Granados Torres, José Enrique|||0000-0002-9787-9896 Serrano Ferron, Emmanuel|||0000-0002-9799-9804 Dellamaria, Debora Casais, Rosa|||0000-0001-8349-5878 Rossi, Luca|||0000-0001-6829-5229 Puigdemont Rodriguez, Anna|||0000-0001-5219-7552 Cano-Manuel, Francisco Javier Fandos, Paulino|||0000-0002-9607-8931 Pérez Jiménez, Jesús María|||0000-0001-9159-0365 Espinosa, José|||0000-0002-9036-1402 Soriguer, Ramón C|||0000-0002-9165-7766 Citterio, Carlo López Olvera, Jorge R.|||0000-0002-2999-3451 |
| author2_role |
author author author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
Veterinària Capra pyrenaica Diagnostic ELISA Sarcoptes scabiei Serum |
| topic |
Veterinària Capra pyrenaica Diagnostic ELISA Sarcoptes scabiei Serum |
| description |
Background: Sarcoptic mange is a contagious skin disease caused by the mite Sarcoptes scabiei, affecting different mammalian species worldwide including the Iberian ibex (Capra pyrenaica), in which mortalities over 90 % of the population have been reported. No efficient diagnostic methods are available for this disease, particularly when there are low mite numbers and mild or no clinical signs. In this study, three enzyme-linked immunosorbent assays (ELISA) developed for dog (ELISA A), Cantabrian chamois (Rupicapra pyrenaica parva) (ELISA B) and Alpine chamois (Rupicapra rupicapra) (ELISA C), were evaluated to detect specific antibodies (IgG) to sarcoptic mange in Iberian ibex sera. Methods: Serum samples from 131 Iberian ibexes (86 healthy and 45 scabietic) were collected from 2005 to 2012 in the Sierra Nevada Natural and National Parks (southern Spain). Based on visual inspection, ibexes were classified into one of three categories, namely healthy (without scabietic compatible lesions), mildly affected (skin lesions over less than 50 % of the body surface) and severely affected (skin lesions over more than 50 % of the body surface). The optimal cut-off point, specificity, sensitivity and the area under the curve (AUC) were calculated, and the agreement between tests was determined. Moreover, differences in the optical density (OD) related to scabies severity have been evaluated for the best test. Results: ELISA C showed better performance than the two other tests, reaching higher values of sensitivity (93.0 %) and specificity (93.5 %) against the visual estimation of the percentage of affected skin, chosen as the gold standard. Significantly higher concentrations of specific antibodies were observed with this test in the mildly and severely infested ibexes than in healthy ones. Conclusions: Our results revealed that ELISA C was an optimal test to diagnose sarcoptic mange in the Iberian ibex. Further studies characterizing immune response during the course of the disease, including spontaneous or drug induced recovery, should follow in order to better understand sarcoptic mange in Iberian ibex populations. |
| publishDate |
2016 |
| dc.date.none.fl_str_mv |
2 2016-01-01 2016 2016-01-01 |
| dc.type.none.fl_str_mv |
Article http://purl.org/coar/resource_type/c_6501 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://ddd.uab.cat/record/203422 https://dx.doi.org/urn:doi:10.1186/s13071-016-1843-4 |
| url |
https://ddd.uab.cat/record/203422 https://dx.doi.org/urn:doi:10.1186/s13071-016-1843-4 |
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Inglés eng |
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Inglés |
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eng |
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Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 CGL2012-40043-C02-02 Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 CGL2016-80543-P |
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open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by/4.0/ |
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info:eu-repo/semantics/openAccess |
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open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by/4.0/ |
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openAccess |
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application/pdf |
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reponame:Dipòsit Digital de Documents de la UAB instname:Universitat Autònoma de Barcelona |
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