MiR-151a: a robust endogenous control for normalizing small extracellular vesicle cargo in human cancer.

Small extracellular vesicles (sEVs) in the blood of cancer patients contain higher amounts of tumor markers than those identified as free-circulating. miRNAs have significant biomedical relevance due to their high stability and feasible detection. However, there is no reliable endogenous control ava...

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Detalles Bibliográficos
Autores: Burdiel, Miranda, Jiménez, Julia, Rodríguez Antolín, Carlos, García Guede, Álvaro, Pernía, Olga, Sastre Perona, Ana, Rosas Alonso, Rocío, Colmenarejo, Julián, Rodríguez-Jiménez, Carmen, Diestro, María Dolores, Martínez Martín, Virginia, Higueras, Oliver, Cruz, Patricia, Losantos García, Itsaso, Peinado, Héctor, De Castro, Javier, Ibáñez de Cáceres, Inmaculada
Tipo de recurso: artículo
Fecha de publicación:2023
País:España
Institución:Universidad Francisco de Vitoria
Repositorio:DDFV. Repositorio Institucional de la Universidad Francisco de Vitoria
Idioma:inglés
OAI Identifier:oai:ddfv.ufv.es:10641/5504
Acceso en línea:https://hdl.handle.net/10641/5504
Access Level:acceso abierto
Palabra clave:miRNAs standardization
miRNA endogenous control in liquid biopsy
Descripción
Sumario:Small extracellular vesicles (sEVs) in the blood of cancer patients contain higher amounts of tumor markers than those identified as free-circulating. miRNAs have significant biomedical relevance due to their high stability and feasible detection. However, there is no reliable endogenous control available to measure sEVs-miRNA content, impairing the acquisition of standardized consistent measurements in cancer liquid biopsy. In this study, we identified three miRNAs from a panel of nine potential normalizers that emerged from a comprehensive analysis comparing the sEVmiRNA profile of six lung and ovarian human cancer cell lines in the absence of or under different conditions. Their relevance as normalizers was tested in 26 additional human cancer cell lines from nine different tumor types undergoing chemotherapy or radiotherapy treatment. The validation cohorts were comprised of 242 prospective plasma and ascitic fluid samples from three different human tumor types. Variability and normalization properties were tested in comparison to miR-16, the most used control to normalize free-circulating miRNAs in plasma. Our results indicate that miR-151a is consistently represented in small extracellular vesicles with minimal variability compared to miR- 16, providing a novel normalizer to measure small extracellular vesicle miRNA content that will benefit liquid biopsy in cancer patients.

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