Molecular evolution and role of aquaporins in the water-to-land transitions of amphibious fishes

Aquaporins (AQPs) or major intrinsic proteins (MIPs) form an ancient family of transporters for water and small solute across biological membranes. They constitute a highly diverse protein superfamily, and their evolutionary history and functions have been relatively well studied in vertebrates and...

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Detalhes bibliográficos
Autor: Lorente Martínez, Héctor
Formato: tesis doctoral
Fecha de publicación:2023
País:España
Recursos:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/87846
Acesso em linha:https://hdl.handle.net/20.500.14352/87846
Access Level:acceso abierto
Palavra-chave:597.6(043.2)
Anfibios
Zoología
2401 Biología Animal (Zoología)
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spelling Molecular evolution and role of aquaporins in the water-to-land transitions of amphibious fishesEvolución molecular y rol de las acuaporinas en las transiciones agua-tierra de los peces anfibiosLorente Martínez, Héctor597.6(043.2)AnfibiosZoología2401 Biología Animal (Zoología)Aquaporins (AQPs) or major intrinsic proteins (MIPs) form an ancient family of transporters for water and small solute across biological membranes. They constitute a highly diverse protein superfamily, and their evolutionary history and functions have been relatively well studied in vertebrates and plants. For instance, in vertebrates four well defined clusters are described: AQP1-like (water-selective classical aquaporins), AQP8-like (ammonia channels), AQP3-like (aquaglyceroporins), and AQP11-like (unorthodox or super aquaporins). In land vertebrates (Tetrapoda), an exclusive clade of MIPs/AQPs—which is clustered within the classical aquaporins and includes three different orthologues—appears to have been important for their process of colonisation of terrestrial environments. MIPs are broadly present across the eukaryotic tree of life suggesting both a more complex evolutionary history and a larger set of functions than previously thought. Here, we studied the diversity of MIP proteins in the entire eukaryotic lineage by setting a general phylogenetic context for understanding their evolution. Besides, considering the importance of aquaporins in the water-to-land transition of sarcopterygian vertebrates (emergence of tetrapods), we studied the molecular evolution of these proteins in several amphibious fishes of the actinopterygian branch in order to investigate possible new duplication events or adaptive modifications at the sequence level that could be related with their acquisition of an amphibious lifestyle. Finally, we provide a robust bioinformatic workflow and pipeline for gene isolation from large-scale genomic data and phylogenetic analyses that enabled the achievement of the molecular evolution goals...Universidad Complutense de MadridAgorreta Calvo, AinhoaSan Mauro Martín, DiegoUniversidad Complutense de Madrid20232023-09-2120232023-09-21doctoral thesishttp://purl.org/coar/resource_type/c_db06info:eu-repo/semantics/doctoralThesisapplication/pdfhttps://hdl.handle.net/20.500.14352/87846reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/878462026-06-02T12:44:21Z
dc.title.none.fl_str_mv Molecular evolution and role of aquaporins in the water-to-land transitions of amphibious fishes
Evolución molecular y rol de las acuaporinas en las transiciones agua-tierra de los peces anfibios
title Molecular evolution and role of aquaporins in the water-to-land transitions of amphibious fishes
spellingShingle Molecular evolution and role of aquaporins in the water-to-land transitions of amphibious fishes
Lorente Martínez, Héctor
597.6(043.2)
Anfibios
Zoología
2401 Biología Animal (Zoología)
title_short Molecular evolution and role of aquaporins in the water-to-land transitions of amphibious fishes
title_full Molecular evolution and role of aquaporins in the water-to-land transitions of amphibious fishes
title_fullStr Molecular evolution and role of aquaporins in the water-to-land transitions of amphibious fishes
title_full_unstemmed Molecular evolution and role of aquaporins in the water-to-land transitions of amphibious fishes
title_sort Molecular evolution and role of aquaporins in the water-to-land transitions of amphibious fishes
dc.creator.none.fl_str_mv Lorente Martínez, Héctor
author Lorente Martínez, Héctor
author_facet Lorente Martínez, Héctor
author_role author
dc.contributor.none.fl_str_mv Agorreta Calvo, Ainhoa
San Mauro Martín, Diego
Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 597.6(043.2)
Anfibios
Zoología
2401 Biología Animal (Zoología)
topic 597.6(043.2)
Anfibios
Zoología
2401 Biología Animal (Zoología)
description Aquaporins (AQPs) or major intrinsic proteins (MIPs) form an ancient family of transporters for water and small solute across biological membranes. They constitute a highly diverse protein superfamily, and their evolutionary history and functions have been relatively well studied in vertebrates and plants. For instance, in vertebrates four well defined clusters are described: AQP1-like (water-selective classical aquaporins), AQP8-like (ammonia channels), AQP3-like (aquaglyceroporins), and AQP11-like (unorthodox or super aquaporins). In land vertebrates (Tetrapoda), an exclusive clade of MIPs/AQPs—which is clustered within the classical aquaporins and includes three different orthologues—appears to have been important for their process of colonisation of terrestrial environments. MIPs are broadly present across the eukaryotic tree of life suggesting both a more complex evolutionary history and a larger set of functions than previously thought. Here, we studied the diversity of MIP proteins in the entire eukaryotic lineage by setting a general phylogenetic context for understanding their evolution. Besides, considering the importance of aquaporins in the water-to-land transition of sarcopterygian vertebrates (emergence of tetrapods), we studied the molecular evolution of these proteins in several amphibious fishes of the actinopterygian branch in order to investigate possible new duplication events or adaptive modifications at the sequence level that could be related with their acquisition of an amphibious lifestyle. Finally, we provide a robust bioinformatic workflow and pipeline for gene isolation from large-scale genomic data and phylogenetic analyses that enabled the achievement of the molecular evolution goals...
publishDate 2023
dc.date.none.fl_str_mv 2023
2023-09-21
2023
2023-09-21
dc.type.none.fl_str_mv doctoral thesis
http://purl.org/coar/resource_type/c_db06
dc.type.openaire.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/87846
url https://hdl.handle.net/20.500.14352/87846
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidad Complutense de Madrid
publisher.none.fl_str_mv Universidad Complutense de Madrid
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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