Yap/Taz-TEAD activity links mechanical cues to progenitor cell behavior during zebrafish hindbrain segmentation

Cells perceive their microenvironment through chemical and physical cues. However, how the mechanical signals are interpreted during embryonic tissue deformation to result in specific cell behaviors is largely unknown. The Yap/Taz family of transcriptional co-activators has emerged as an important r...

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Detalhes bibliográficos
Autores: Voltes Cobo, Adrià, 1991-, Hevia, Covadonga F., Engel-Pizcueta, Carolyn, Dingare, Chaitanya, Calzolari, Simone, 1983-, Terriente, Javier, Norden, Caren, Lecaudey, Virginie, Pujades Corbi, Cristina
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2019
País:España
Recursos:Universitat Pompeu Fabra
Repositório:Repositorio Digital de la UPF
OAI Identifier:oai:repositori.upf.edu:10230/42278
Acesso em linha:http://hdl.handle.net/10230/42278
http://dx.doi.org/10.1242/dev.176735
Access Level:Acceso aberto
Palavra-chave:Boundaries
Compartments
Hindbrain
Mechanical cues
Neurons
Progenitor cells
Yap/Taz
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spelling Yap/Taz-TEAD activity links mechanical cues to progenitor cell behavior during zebrafish hindbrain segmentationVoltes Cobo, Adrià, 1991-Hevia, Covadonga F.Engel-Pizcueta, CarolynDingare, ChaitanyaCalzolari, Simone, 1983-Terriente, JavierNorden, CarenLecaudey, VirginiePujades Corbi, CristinaBoundariesCompartmentsHindbrainMechanical cuesNeuronsProgenitor cellsYap/TazCells perceive their microenvironment through chemical and physical cues. However, how the mechanical signals are interpreted during embryonic tissue deformation to result in specific cell behaviors is largely unknown. The Yap/Taz family of transcriptional co-activators has emerged as an important regulator of tissue growth and regeneration, responding to physical cues from the extracellular matrix, and to cell shape and actomyosin cytoskeletal changes. In this study, we demonstrate the role of Yap/Taz-TEAD activity as a sensor of mechanical signals in the regulation of the progenitor behavior of boundary cells during zebrafish hindbrain compartmentalization. Monitoring of in vivo Yap/Taz activity during hindbrain segmentation indicated that boundary cells responded to mechanical cues in a cell-autonomous manner through Yap/Taz-TEAD activity. Cell-lineage analysis revealed that Yap/Taz-TEAD boundary cells decreased their proliferative activity when Yap/Taz-TEAD activity ceased, which preceded changes in their cell fate from proliferating progenitors to differentiated neurons. Functional experiments demonstrated the pivotal role of Yap/Taz-TEAD signaling in maintaining progenitor features in the hindbrain boundary cell population.This work was supported by a La Marató-TV3 grant (345/C/2014) and by a Ministerio de Ciencia, Innovación y Universidades grant (BFU2015-67400-P and BFU2016-81887-REDT/AEI) to C.P.; and by a Unidad de Excelencia María de Maetzu grant (MDM-2014-0370) to the Department of Experimental and Health Sciences of the Pompeu Fabra University (DCEXS-UPF). A.V. was a recipient of a predoctoral fellowship from the Fundació La Caixa, and C.E.-P. holds a predoctoral fellowship from the Ministerio de Ciencia, Innovación y Universidades (FPU). J.T. was a recipient of a postdoctoral Beatriu de Pinos fellowship (AGAUR, Generalitat de Catalunya). C.P. is recipient of an Institució Catalana per la Recerca i Estudis Avançats Academia award (Generalitat de Catalunya).Company of Biologists20192019info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/42278http://dx.doi.org/10.1242/dev.176735reponame:Repositorio Digital de la UPFinstname:Universitat Pompeu FabraInglésDevelopment. 2019;146(14):dev176735info:eu-repo/grantAgreement/ES/1PE/BFU2015-67400-Pinfo:eu-repo/grantAgreement/ES/1PE/BFU2016-81887-REDT© Company of Biologists https://dev.biologists.org/content/146/14/dev176735 DOI 10.1242/dev.176735info:eu-repo/semantics/openAccessoai:repositori.upf.edu:10230/422782026-06-12T07:21:37Z
dc.title.none.fl_str_mv Yap/Taz-TEAD activity links mechanical cues to progenitor cell behavior during zebrafish hindbrain segmentation
title Yap/Taz-TEAD activity links mechanical cues to progenitor cell behavior during zebrafish hindbrain segmentation
spellingShingle Yap/Taz-TEAD activity links mechanical cues to progenitor cell behavior during zebrafish hindbrain segmentation
Voltes Cobo, Adrià, 1991-
Boundaries
Compartments
Hindbrain
Mechanical cues
Neurons
Progenitor cells
Yap/Taz
title_short Yap/Taz-TEAD activity links mechanical cues to progenitor cell behavior during zebrafish hindbrain segmentation
title_full Yap/Taz-TEAD activity links mechanical cues to progenitor cell behavior during zebrafish hindbrain segmentation
title_fullStr Yap/Taz-TEAD activity links mechanical cues to progenitor cell behavior during zebrafish hindbrain segmentation
title_full_unstemmed Yap/Taz-TEAD activity links mechanical cues to progenitor cell behavior during zebrafish hindbrain segmentation
title_sort Yap/Taz-TEAD activity links mechanical cues to progenitor cell behavior during zebrafish hindbrain segmentation
dc.creator.none.fl_str_mv Voltes Cobo, Adrià, 1991-
Hevia, Covadonga F.
Engel-Pizcueta, Carolyn
Dingare, Chaitanya
Calzolari, Simone, 1983-
Terriente, Javier
Norden, Caren
Lecaudey, Virginie
Pujades Corbi, Cristina
author Voltes Cobo, Adrià, 1991-
author_facet Voltes Cobo, Adrià, 1991-
Hevia, Covadonga F.
Engel-Pizcueta, Carolyn
Dingare, Chaitanya
Calzolari, Simone, 1983-
Terriente, Javier
Norden, Caren
Lecaudey, Virginie
Pujades Corbi, Cristina
author_role author
author2 Hevia, Covadonga F.
Engel-Pizcueta, Carolyn
Dingare, Chaitanya
Calzolari, Simone, 1983-
Terriente, Javier
Norden, Caren
Lecaudey, Virginie
Pujades Corbi, Cristina
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Boundaries
Compartments
Hindbrain
Mechanical cues
Neurons
Progenitor cells
Yap/Taz
topic Boundaries
Compartments
Hindbrain
Mechanical cues
Neurons
Progenitor cells
Yap/Taz
description Cells perceive their microenvironment through chemical and physical cues. However, how the mechanical signals are interpreted during embryonic tissue deformation to result in specific cell behaviors is largely unknown. The Yap/Taz family of transcriptional co-activators has emerged as an important regulator of tissue growth and regeneration, responding to physical cues from the extracellular matrix, and to cell shape and actomyosin cytoskeletal changes. In this study, we demonstrate the role of Yap/Taz-TEAD activity as a sensor of mechanical signals in the regulation of the progenitor behavior of boundary cells during zebrafish hindbrain compartmentalization. Monitoring of in vivo Yap/Taz activity during hindbrain segmentation indicated that boundary cells responded to mechanical cues in a cell-autonomous manner through Yap/Taz-TEAD activity. Cell-lineage analysis revealed that Yap/Taz-TEAD boundary cells decreased their proliferative activity when Yap/Taz-TEAD activity ceased, which preceded changes in their cell fate from proliferating progenitors to differentiated neurons. Functional experiments demonstrated the pivotal role of Yap/Taz-TEAD signaling in maintaining progenitor features in the hindbrain boundary cell population.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10230/42278
http://dx.doi.org/10.1242/dev.176735
url http://hdl.handle.net/10230/42278
http://dx.doi.org/10.1242/dev.176735
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Development. 2019;146(14):dev176735
info:eu-repo/grantAgreement/ES/1PE/BFU2015-67400-P
info:eu-repo/grantAgreement/ES/1PE/BFU2016-81887-REDT
dc.rights.none.fl_str_mv © Company of Biologists https://dev.biologists.org/content/146/14/dev176735 DOI 10.1242/dev.176735
info:eu-repo/semantics/openAccess
rights_invalid_str_mv © Company of Biologists https://dev.biologists.org/content/146/14/dev176735 DOI 10.1242/dev.176735
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Company of Biologists
publisher.none.fl_str_mv Company of Biologists
dc.source.none.fl_str_mv reponame:Repositorio Digital de la UPF
instname:Universitat Pompeu Fabra
instname_str Universitat Pompeu Fabra
reponame_str Repositorio Digital de la UPF
collection Repositorio Digital de la UPF
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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