Diversity of Vibrionaceae bacteria isolated from the haemolymph of the spider crab Maja brachydactyla

Aims: The aim of this study was to characterise and identify vibrios isolated from the haemolymph of apparently healthy adult spider crabs (Maja brachydactyla) wild-caught in the Spanish localities of Galician coast and in the Canary Islands and also from captive animals held at IRTA’s facilities in...

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Autores: Gomez-Gil, Bruno, Roque, A., Lacuesta, B., Rotllant Estelrich, Guiomar
Tipo de recurso: artículo
Fecha de publicación:2010
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:20.500.12327/4504
Acceso en línea:http://hdl.handle.net/20.500.12327/4504
Access Level:acceso abierto
Palabra clave:Crustacis -- Conservació
63
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spelling Diversity of Vibrionaceae bacteria isolated from the haemolymph of the spider crab Maja brachydactylaGomez-Gil, BrunoRoque, A.Lacuesta, B.Rotllant Estelrich, GuiomarCrustacis -- Conservació63Aims: The aim of this study was to characterise and identify vibrios isolated from the haemolymph of apparently healthy adult spider crabs (Maja brachydactyla) wild-caught in the Spanish localities of Galician coast and in the Canary Islands and also from captive animals held at IRTA’s facilities in the Ebro Delta of Catalonia, north-west Spanish Mediterranean coast. Methods and Results: A total of 277 bacterial isolates were obtained, and of these, 171 were characterised with rep-PCR, resulting electrophoretic bands were analysed and clusters formed. Identification of representative strains of each cluster was made by sequencing the 16S rRNA. Samples from animals caught in Galicia and captive at IRTA (around 15–18 C) rendered mostly species belonging to the Splendidus clade (72Æ2 and 76Æ6% respectively), commonly found in cold waters (below 20 C). Higher species diversity was found in the haemolymph of the captive animals. In the warmer Canary Islands waters (around 21 C), the diversity of vibrios is dominated by three clades, Harveyi (Vibrio core group, 39Æ3%), Orientalis (23Æ2%) and Splendidus (21Æ4%) with a species diversity that equals that of the colder captive animals. Conclusions: Differences in the vibrios populations were found in the haemolymph extracted from animals collected from the three localities. Potential new species were found, and their description is under way. Significance and Impact of Study: As with other invertebrates, spider crabs also contain a diverse population of vibrios. These findings should help researchers to diagnose when a crab is infected.Wiley-BlackwellProducció AnimalAqüiculturaIRTA. Recerca i Tecnologia Agroalimentàries2010info:eu-repo/semantics/article28 p.http://hdl.handle.net/20.500.12327/4504reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a:http://dx.doi.org/10.1111/j.1365-2672.2010.04718.xinfo:eu-repo/semantics/openAccessL'accés als continguts d'aquest document queda condicionat a l'acceptació de les condicions d'ús establertes per la següent llicència Creative Commons: http://creativecommons.org/licenses/by-nc-sa/3.0/es/ oai:recercat.cat:20.500.12327/45042026-05-29T05:05:01Z
dc.title.none.fl_str_mv Diversity of Vibrionaceae bacteria isolated from the haemolymph of the spider crab Maja brachydactyla
title Diversity of Vibrionaceae bacteria isolated from the haemolymph of the spider crab Maja brachydactyla
spellingShingle Diversity of Vibrionaceae bacteria isolated from the haemolymph of the spider crab Maja brachydactyla
Gomez-Gil, Bruno
Crustacis -- Conservació
63
title_short Diversity of Vibrionaceae bacteria isolated from the haemolymph of the spider crab Maja brachydactyla
title_full Diversity of Vibrionaceae bacteria isolated from the haemolymph of the spider crab Maja brachydactyla
title_fullStr Diversity of Vibrionaceae bacteria isolated from the haemolymph of the spider crab Maja brachydactyla
title_full_unstemmed Diversity of Vibrionaceae bacteria isolated from the haemolymph of the spider crab Maja brachydactyla
title_sort Diversity of Vibrionaceae bacteria isolated from the haemolymph of the spider crab Maja brachydactyla
dc.creator.none.fl_str_mv Gomez-Gil, Bruno
Roque, A.
Lacuesta, B.
Rotllant Estelrich, Guiomar
author Gomez-Gil, Bruno
author_facet Gomez-Gil, Bruno
Roque, A.
Lacuesta, B.
Rotllant Estelrich, Guiomar
author_role author
author2 Roque, A.
Lacuesta, B.
Rotllant Estelrich, Guiomar
author2_role author
author
author
dc.contributor.none.fl_str_mv Producció Animal
Aqüicultura
IRTA. Recerca i Tecnologia Agroalimentàries
dc.subject.none.fl_str_mv Crustacis -- Conservació
63
topic Crustacis -- Conservació
63
description Aims: The aim of this study was to characterise and identify vibrios isolated from the haemolymph of apparently healthy adult spider crabs (Maja brachydactyla) wild-caught in the Spanish localities of Galician coast and in the Canary Islands and also from captive animals held at IRTA’s facilities in the Ebro Delta of Catalonia, north-west Spanish Mediterranean coast. Methods and Results: A total of 277 bacterial isolates were obtained, and of these, 171 were characterised with rep-PCR, resulting electrophoretic bands were analysed and clusters formed. Identification of representative strains of each cluster was made by sequencing the 16S rRNA. Samples from animals caught in Galicia and captive at IRTA (around 15–18 C) rendered mostly species belonging to the Splendidus clade (72Æ2 and 76Æ6% respectively), commonly found in cold waters (below 20 C). Higher species diversity was found in the haemolymph of the captive animals. In the warmer Canary Islands waters (around 21 C), the diversity of vibrios is dominated by three clades, Harveyi (Vibrio core group, 39Æ3%), Orientalis (23Æ2%) and Splendidus (21Æ4%) with a species diversity that equals that of the colder captive animals. Conclusions: Differences in the vibrios populations were found in the haemolymph extracted from animals collected from the three localities. Potential new species were found, and their description is under way. Significance and Impact of Study: As with other invertebrates, spider crabs also contain a diverse population of vibrios. These findings should help researchers to diagnose when a crab is infected.
publishDate 2010
dc.date.none.fl_str_mv 2010
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12327/4504
url http://hdl.handle.net/20.500.12327/4504
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a:http://dx.doi.org/10.1111/j.1365-2672.2010.04718.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 28 p.
dc.publisher.none.fl_str_mv Wiley-Blackwell
publisher.none.fl_str_mv Wiley-Blackwell
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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