Metabarcoding analysis on European coastal samples reveals new molecular metazoan diversity

Although animals are among the best studied organisms, we still lack a full description of their diversity, especially for microscopic taxa. This is partly due to the time-consuming and costly nature of surveying animal diversity through morphological and molecular studies of individual taxa. A powe...

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Autores: López Escardó, David, 1988-, Paps, Jordi, Vargas, Colomban de, Massana, Ramon, Ruiz Trillo, Iñaki, Campo, Javier del
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2018
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10230/35645
Acceso en línea:http://hdl.handle.net/10230/35645
http://dx.doi.org/10.1038/s41598-018-27509-8
Access Level:acceso abierto
Palabra clave:Metazous
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spelling Metabarcoding analysis on European coastal samples reveals new molecular metazoan diversityLópez Escardó, David, 1988-Paps, JordiVargas, Colomban deMassana, RamonRuiz Trillo, IñakiCampo, Javier delMetazousAlthough animals are among the best studied organisms, we still lack a full description of their diversity, especially for microscopic taxa. This is partly due to the time-consuming and costly nature of surveying animal diversity through morphological and molecular studies of individual taxa. A powerful alternative is the use of high-throughput environmental sequencing, providing molecular data from all organisms sampled. We here address the unknown diversity of animal phyla in marine environments using an extensive dataset designed to assess eukaryotic ribosomal diversity among European coastal locations. A multi-phylum assessment of marine animal diversity that includes water column and sediments, oxic and anoxic environments, and both DNA and RNA templates, revealed a high percentage of novel 18S rRNA sequences in most phyla, suggesting that marine environments have not yet been fully sampled at a molecular level. This novelty is especially high among Platyhelminthes, Acoelomorpha, and Nematoda, which are well studied from a morphological perspective and abundant in benthic environments. We also identified, based on molecular data, a potentially novel group of widespread tunicates. Moreover, we recovered a high number of reads for Ctenophora and Cnidaria in the smaller fractions suggesting their gametes might play a greater ecological role than previously suspected.This work was supported by an Institució Catalana de Recerca i Estudis Avançats contract, two grants (BFU-2011-23434 and BFU2014-57779-P) from the Ministerio de Economia y Competitividad (MINECO), one of which (BFU2014-57779-P) was co-funded by the European Regional Development Fund (FEDER), and a European Research Council Consolidator Grant (ERC-2012-Co -616960) to I.R.-T. We also acknowledge financial support from the Secretaria d’Universitats i Recerca del Departament d’Economia i Coneixement de la Generalitat de Catalunya (Project 2014 SGR 619). J.d.C. was supported by a Marie Curie International Outgoing Fellowship grant (FP7-PEOPLE-2012-IOF - 331450 CAARL). J.P. acknowledges support from the European Research Council under the European Union’s Seventh Framework Program (FP7/2007–2013)/ERC grant [268513]. The work is part of the EU ERA-Net program BiodivERsA, under the project BioMarKs (Biodiversity of Marine euKaryotes)Nature Publishing Group201820182018info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/35645http://dx.doi.org/10.1038/s41598-018-27509-8reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésScientific Reports. 2018 Jun 14;8(1):9106info:eu-repo/grantAgreement/EC/FP7/616960info:eu-repo/grantAgreement/EC/FP7/331450info:eu-repo/grantAgreement/EC/FP7/268513© David López-Escardó et al 2018. This article is licensed under a Creative Commons Attribution 4.0 International Licensehttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:10230/356452026-05-29T05:05:01Z
dc.title.none.fl_str_mv Metabarcoding analysis on European coastal samples reveals new molecular metazoan diversity
title Metabarcoding analysis on European coastal samples reveals new molecular metazoan diversity
spellingShingle Metabarcoding analysis on European coastal samples reveals new molecular metazoan diversity
López Escardó, David, 1988-
Metazous
title_short Metabarcoding analysis on European coastal samples reveals new molecular metazoan diversity
title_full Metabarcoding analysis on European coastal samples reveals new molecular metazoan diversity
title_fullStr Metabarcoding analysis on European coastal samples reveals new molecular metazoan diversity
title_full_unstemmed Metabarcoding analysis on European coastal samples reveals new molecular metazoan diversity
title_sort Metabarcoding analysis on European coastal samples reveals new molecular metazoan diversity
dc.creator.none.fl_str_mv López Escardó, David, 1988-
Paps, Jordi
Vargas, Colomban de
Massana, Ramon
Ruiz Trillo, Iñaki
Campo, Javier del
author López Escardó, David, 1988-
author_facet López Escardó, David, 1988-
Paps, Jordi
Vargas, Colomban de
Massana, Ramon
Ruiz Trillo, Iñaki
Campo, Javier del
author_role author
author2 Paps, Jordi
Vargas, Colomban de
Massana, Ramon
Ruiz Trillo, Iñaki
Campo, Javier del
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Metazous
topic Metazous
description Although animals are among the best studied organisms, we still lack a full description of their diversity, especially for microscopic taxa. This is partly due to the time-consuming and costly nature of surveying animal diversity through morphological and molecular studies of individual taxa. A powerful alternative is the use of high-throughput environmental sequencing, providing molecular data from all organisms sampled. We here address the unknown diversity of animal phyla in marine environments using an extensive dataset designed to assess eukaryotic ribosomal diversity among European coastal locations. A multi-phylum assessment of marine animal diversity that includes water column and sediments, oxic and anoxic environments, and both DNA and RNA templates, revealed a high percentage of novel 18S rRNA sequences in most phyla, suggesting that marine environments have not yet been fully sampled at a molecular level. This novelty is especially high among Platyhelminthes, Acoelomorpha, and Nematoda, which are well studied from a morphological perspective and abundant in benthic environments. We also identified, based on molecular data, a potentially novel group of widespread tunicates. Moreover, we recovered a high number of reads for Ctenophora and Cnidaria in the smaller fractions suggesting their gametes might play a greater ecological role than previously suspected.
publishDate 2018
dc.date.none.fl_str_mv 2018
2018
2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10230/35645
http://dx.doi.org/10.1038/s41598-018-27509-8
url http://hdl.handle.net/10230/35645
http://dx.doi.org/10.1038/s41598-018-27509-8
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Scientific Reports. 2018 Jun 14;8(1):9106
info:eu-repo/grantAgreement/EC/FP7/616960
info:eu-repo/grantAgreement/EC/FP7/331450
info:eu-repo/grantAgreement/EC/FP7/268513
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
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