IGF-1 deficiency causes atrophic changes associated with upregulation of VGluT1 and downregulation of MEF2 transcription factors in the mouse cochlear nuclei.

Fuentes Santamaría, Verónica; Alvarado Romero, Juan Carlos; Rodríguez de la Rosa, Lourdes; Murillo Cuesta, Silvia; Contreras, J.; Juiz Gómez, José Manuel; Varela Nieto, Isabel

IGF-1 deficiency causes atrophic changes associated with upregulation of VGluT1 and downregulation of MEF2 transcription factors in the mouse cochlear nuclei.

Insulin-like growth factor 1 (IGF-1) is a neurotrophic protein that plays a crucial role in modulating neuronal function and synaptic plasticity in the adult brain. Mice lacking the Igf1 gene exhibit profound deafness and multiple anomalies in the inner ear and spiral ganglion. An issue that remains...

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Autores:	Fuentes Santamaría, Verónica, Alvarado Romero, Juan Carlos, Rodríguez de la Rosa, Lourdes, Murillo Cuesta, Silvia, Contreras, J., Juiz Gómez, José Manuel, Varela Nieto, Isabel
Tipo de recurso:	artículo
Fecha de publicación:	2016
País:	España
Institución:	Universidad de Castilla-La Mancha
Repositorio:	RUIdeRA. Repositorio Institucional de la UCLM
OAI Identifier:	oai:ruidera.uclm.es:10578/33187
Acceso en línea:	https://link.springer.com/article/10.1007/s00429-014-0934-2 https://hdl.handle.net/10578/33187
Access Level:	acceso abierto
Palabra clave:	Auditory Cochlear nucleus Deafness IGF-1 Image analysis

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spelling	IGF-1 deficiency causes atrophic changes associated with upregulation of VGluT1 and downregulation of MEF2 transcription factors in the mouse cochlear nuclei.Fuentes Santamaría, VerónicaAlvarado Romero, Juan CarlosRodríguez de la Rosa, LourdesMurillo Cuesta, SilviaContreras, J.Juiz Gómez, José ManuelVarela Nieto, IsabelAuditoryCochlear nucleusDeafnessIGF-1Image analysisInsulin-like growth factor 1 (IGF-1) is a neurotrophic protein that plays a crucial role in modulating neuronal function and synaptic plasticity in the adult brain. Mice lacking the Igf1 gene exhibit profound deafness and multiple anomalies in the inner ear and spiral ganglion. An issue that remains unknown is whether, in addition to these peripheral abnormalities, IGF-1 deficiency also results in structural changes along the central auditory pathway that may contribute to an imbalance between excitation and inhibition, which might be reflected in abnormal auditory brainstem responses (ABR). To assess such a possibility, we evaluated the morphological and physiological alterations in the cochlear nucleus complex of the adult mouse. The expression and distribution of the vesicular glutamate transporter 1 (VGluT1) and the vesicular inhibitory transporter (VGAT), which were used as specific markers for labeling excitatory and inhibitory terminals, and the involvement of the activity-dependent myocyte enhancer factor 2 (MEF2) transcription factors in regulating excitatory synapses were assessed in a 4-month-old mouse model of IGF-1 deficiency and neurosensorial deafness (Igf1 -/- homozygous null mice). The results demonstrate decreases in the cochlear nucleus area and cell size along with cell loss in the cochlear nuclei of the deficient mouse. Additionally, our results demonstrate that there is upregulation of VGluT1, but not VGAT, immunostaining and downregulation of MEF2 transcription factors together with increased wave II amplitude in the ABR recording. Our observations provide evidence of an abnormal neuronal cytoarchitecture in the cochlear nuclei of Igf1 -/- null mice and suggest that the increased efficacy of glutamatergic synapses might be mediated by MEF2 transcription factors.Springer202420242016info:eu-repo/semantics/articleapplication/pdfapplication/pdfhttps://link.springer.com/article/10.1007/s00429-014-0934-2https://hdl.handle.net/10578/33187reponame:RUIdeRA. Repositorio Institucional de la UCLMinstname:Universidad de Castilla-La ManchaInglésSAF2011-24391FP7-HEALTH2012-INNOVATION-2 304900 AFHELOFP7-PEOPLE-2013-IAPP 612261TARGEARBFU2009-13754-C02-01FP7-HEALTH-2012-INNOVATION 304925info:eu-repo/semantics/openAccessoai:ruidera.uclm.es:10578/331872026-05-27T07:36:41Z
dc.title.none.fl_str_mv	IGF-1 deficiency causes atrophic changes associated with upregulation of VGluT1 and downregulation of MEF2 transcription factors in the mouse cochlear nuclei.
title	IGF-1 deficiency causes atrophic changes associated with upregulation of VGluT1 and downregulation of MEF2 transcription factors in the mouse cochlear nuclei.
spellingShingle	IGF-1 deficiency causes atrophic changes associated with upregulation of VGluT1 and downregulation of MEF2 transcription factors in the mouse cochlear nuclei. Fuentes Santamaría, Verónica Auditory Cochlear nucleus Deafness IGF-1 Image analysis
title_short	IGF-1 deficiency causes atrophic changes associated with upregulation of VGluT1 and downregulation of MEF2 transcription factors in the mouse cochlear nuclei.
title_full	IGF-1 deficiency causes atrophic changes associated with upregulation of VGluT1 and downregulation of MEF2 transcription factors in the mouse cochlear nuclei.
title_fullStr	IGF-1 deficiency causes atrophic changes associated with upregulation of VGluT1 and downregulation of MEF2 transcription factors in the mouse cochlear nuclei.
title_full_unstemmed	IGF-1 deficiency causes atrophic changes associated with upregulation of VGluT1 and downregulation of MEF2 transcription factors in the mouse cochlear nuclei.
title_sort	IGF-1 deficiency causes atrophic changes associated with upregulation of VGluT1 and downregulation of MEF2 transcription factors in the mouse cochlear nuclei.
dc.creator.none.fl_str_mv	Fuentes Santamaría, Verónica Alvarado Romero, Juan Carlos Rodríguez de la Rosa, Lourdes Murillo Cuesta, Silvia Contreras, J. Juiz Gómez, José Manuel Varela Nieto, Isabel
author	Fuentes Santamaría, Verónica
author_facet	Fuentes Santamaría, Verónica Alvarado Romero, Juan Carlos Rodríguez de la Rosa, Lourdes Murillo Cuesta, Silvia Contreras, J. Juiz Gómez, José Manuel Varela Nieto, Isabel
author_role	author
author2	Alvarado Romero, Juan Carlos Rodríguez de la Rosa, Lourdes Murillo Cuesta, Silvia Contreras, J. Juiz Gómez, José Manuel Varela Nieto, Isabel
author2_role	author author author author author author
dc.subject.none.fl_str_mv	Auditory Cochlear nucleus Deafness IGF-1 Image analysis
topic	Auditory Cochlear nucleus Deafness IGF-1 Image analysis
description	Insulin-like growth factor 1 (IGF-1) is a neurotrophic protein that plays a crucial role in modulating neuronal function and synaptic plasticity in the adult brain. Mice lacking the Igf1 gene exhibit profound deafness and multiple anomalies in the inner ear and spiral ganglion. An issue that remains unknown is whether, in addition to these peripheral abnormalities, IGF-1 deficiency also results in structural changes along the central auditory pathway that may contribute to an imbalance between excitation and inhibition, which might be reflected in abnormal auditory brainstem responses (ABR). To assess such a possibility, we evaluated the morphological and physiological alterations in the cochlear nucleus complex of the adult mouse. The expression and distribution of the vesicular glutamate transporter 1 (VGluT1) and the vesicular inhibitory transporter (VGAT), which were used as specific markers for labeling excitatory and inhibitory terminals, and the involvement of the activity-dependent myocyte enhancer factor 2 (MEF2) transcription factors in regulating excitatory synapses were assessed in a 4-month-old mouse model of IGF-1 deficiency and neurosensorial deafness (Igf1 -/- homozygous null mice). The results demonstrate decreases in the cochlear nucleus area and cell size along with cell loss in the cochlear nuclei of the deficient mouse. Additionally, our results demonstrate that there is upregulation of VGluT1, but not VGAT, immunostaining and downregulation of MEF2 transcription factors together with increased wave II amplitude in the ABR recording. Our observations provide evidence of an abnormal neuronal cytoarchitecture in the cochlear nuclei of Igf1 -/- null mice and suggest that the increased efficacy of glutamatergic synapses might be mediated by MEF2 transcription factors.
publishDate	2016
dc.date.none.fl_str_mv	2016 2024 2024
dc.type.none.fl_str_mv	info:eu-repo/semantics/article
format	article
dc.identifier.none.fl_str_mv	https://link.springer.com/article/10.1007/s00429-014-0934-2 https://hdl.handle.net/10578/33187
url	https://link.springer.com/article/10.1007/s00429-014-0934-2 https://hdl.handle.net/10578/33187
dc.language.none.fl_str_mv	Inglés
language_invalid_str_mv	Inglés
dc.relation.none.fl_str_mv	SAF2011-24391 FP7-HEALTH2012-INNOVATION-2 304900 AFHELO FP7-PEOPLE-2013-IAPP 612261TARGEAR BFU2009-13754-C02-01 FP7-HEALTH-2012-INNOVATION 304925
dc.rights.none.fl_str_mv	info:eu-repo/semantics/openAccess
eu_rights_str_mv	openAccess
dc.format.none.fl_str_mv	application/pdf application/pdf
dc.publisher.none.fl_str_mv	Springer
publisher.none.fl_str_mv	Springer
dc.source.none.fl_str_mv	reponame:RUIdeRA. Repositorio Institucional de la UCLM instname:Universidad de Castilla-La Mancha
instname_str	Universidad de Castilla-La Mancha
reponame_str	RUIdeRA. Repositorio Institucional de la UCLM
collection	RUIdeRA. Repositorio Institucional de la UCLM
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score	15,301603

IGF-1 deficiency causes atrophic changes associated with upregulation of VGluT1 and downregulation of MEF2 transcription factors in the mouse cochlear nuclei.

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