Validation of miRNA-mRNA interactions by electrophoretic mobility shift assays

BACKGROUND: MicroRNAs are small non-coding RNAs involved in gene expression regulation by targeting specific regions in the 3[prime]-UTR of the mRNA of their target genes. This binding leads to a decrease in the protein levels of such genes either by mRNA degradation or mRNA destabilization and tran...

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Detalles Bibliográficos
Autores: Solé Ferré, Anna, Mencía Trinchant, Núria, Villalobos Alberú, Xenia, Noé Mata, Verónica, Ciudad i Gómez, Carlos Julián
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2013
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/177048
Acceso en línea:https://hdl.handle.net/2445/177048
Access Level:acceso abierto
Palabra clave:Micro RNAs
RNA
Dianes farmacològiques
Expressió gènica
MicroRNAs
Drug targeting
Gene expression
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spelling Validation of miRNA-mRNA interactions by electrophoretic mobility shift assaysSolé Ferré, AnnaMencía Trinchant, NúriaVillalobos Alberú, XeniaNoé Mata, VerónicaCiudad i Gómez, Carlos JuliánMicro RNAsRNADianes farmacològiquesExpressió gènicaMicroRNAsRNADrug targetingGene expressionBACKGROUND: MicroRNAs are small non-coding RNAs involved in gene expression regulation by targeting specific regions in the 3[prime]-UTR of the mRNA of their target genes. This binding leads to a decrease in the protein levels of such genes either by mRNA degradation or mRNA destabilization and translation inhibition. The interaction between a miRNA and its target mRNAs is usually studied by co-transfection of a reporter expression vector containing the 3[prime]-UTR region of the mRNA and an inhibitory or precursor molecule for the miRNA. This approach, however, does not measure the direct and physical interaction between a miRNA and a specific mRNA. FINDINGS: RNA molecules corresponding to miR-224 and to the 3[prime]-UTR of SLC4A4 were incubated together and their interaction studied under different binding conditions using electrophoretic mobility shift assays. A direct and specific interaction between miR-224 and SLC4A4 mRNA was observed. This interaction was abolished in the presence of competitors. CONCLUSIONS: In this study, we explored a new application for the electrophoretic mobility shift assay and we demonstrated that it is a useful alternative method to assess, in a direct and specific manner, whether a miRNA binds to a specific predicted target mRNA.BioMed Central2021202120132021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/2445/177048Articles publicats en revistes (Bioquímica i Fisiologia)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.1186/1756-0500-6-454BMC Research Notes, 2013, vol. 6, num. 1, p. 454https://doi.org/10.1186/1756-0500-6-454cc-by (c) Solé Ferré, Anna et al., 2013http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:recercat.cat:2445/1770482026-05-29T05:05:01Z
dc.title.none.fl_str_mv Validation of miRNA-mRNA interactions by electrophoretic mobility shift assays
title Validation of miRNA-mRNA interactions by electrophoretic mobility shift assays
spellingShingle Validation of miRNA-mRNA interactions by electrophoretic mobility shift assays
Solé Ferré, Anna
Micro RNAs
RNA
Dianes farmacològiques
Expressió gènica
MicroRNAs
RNA
Drug targeting
Gene expression
title_short Validation of miRNA-mRNA interactions by electrophoretic mobility shift assays
title_full Validation of miRNA-mRNA interactions by electrophoretic mobility shift assays
title_fullStr Validation of miRNA-mRNA interactions by electrophoretic mobility shift assays
title_full_unstemmed Validation of miRNA-mRNA interactions by electrophoretic mobility shift assays
title_sort Validation of miRNA-mRNA interactions by electrophoretic mobility shift assays
dc.creator.none.fl_str_mv Solé Ferré, Anna
Mencía Trinchant, Núria
Villalobos Alberú, Xenia
Noé Mata, Verónica
Ciudad i Gómez, Carlos Julián
author Solé Ferré, Anna
author_facet Solé Ferré, Anna
Mencía Trinchant, Núria
Villalobos Alberú, Xenia
Noé Mata, Verónica
Ciudad i Gómez, Carlos Julián
author_role author
author2 Mencía Trinchant, Núria
Villalobos Alberú, Xenia
Noé Mata, Verónica
Ciudad i Gómez, Carlos Julián
author2_role author
author
author
author
dc.subject.none.fl_str_mv Micro RNAs
RNA
Dianes farmacològiques
Expressió gènica
MicroRNAs
RNA
Drug targeting
Gene expression
topic Micro RNAs
RNA
Dianes farmacològiques
Expressió gènica
MicroRNAs
RNA
Drug targeting
Gene expression
description BACKGROUND: MicroRNAs are small non-coding RNAs involved in gene expression regulation by targeting specific regions in the 3[prime]-UTR of the mRNA of their target genes. This binding leads to a decrease in the protein levels of such genes either by mRNA degradation or mRNA destabilization and translation inhibition. The interaction between a miRNA and its target mRNAs is usually studied by co-transfection of a reporter expression vector containing the 3[prime]-UTR region of the mRNA and an inhibitory or precursor molecule for the miRNA. This approach, however, does not measure the direct and physical interaction between a miRNA and a specific mRNA. FINDINGS: RNA molecules corresponding to miR-224 and to the 3[prime]-UTR of SLC4A4 were incubated together and their interaction studied under different binding conditions using electrophoretic mobility shift assays. A direct and specific interaction between miR-224 and SLC4A4 mRNA was observed. This interaction was abolished in the presence of competitors. CONCLUSIONS: In this study, we explored a new application for the electrophoretic mobility shift assay and we demonstrated that it is a useful alternative method to assess, in a direct and specific manner, whether a miRNA binds to a specific predicted target mRNA.
publishDate 2013
dc.date.none.fl_str_mv 2013
2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/177048
url https://hdl.handle.net/2445/177048
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1186/1756-0500-6-454
BMC Research Notes, 2013, vol. 6, num. 1, p. 454
https://doi.org/10.1186/1756-0500-6-454
dc.rights.none.fl_str_mv cc-by (c) Solé Ferré, Anna et al., 2013
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Solé Ferré, Anna et al., 2013
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv Articles publicats en revistes (Bioquímica i Fisiologia)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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