α-Haemoglobin regulates sympathoadrenal cell metabolism to maintain a catecholaminergic phenotype

23 páginas, 7 figuras, 2 tablas.

Bibliographic Details
Authors: Marcos-Almaraz, María T., Rodríguez-Gómez, José A., López-Barneo, José, Pascual Bravo, Alberto
Format: article
Publication Date:2011
Country:España
Institution:Consejo Superior de Investigaciones Científicas (CSIC)
Repository:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/49170
Online Access:http://hdl.handle.net/10261/49170
Access Level:Open access
Keyword:Dopamine
Haemoglobin
Oxidative stress
Sympahtoadrenal lineage
Tyrosine hydroxylase
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spelling α-Haemoglobin regulates sympathoadrenal cell metabolism to maintain a catecholaminergic phenotypeMarcos-Almaraz, María T.Rodríguez-Gómez, José A.López-Barneo, JoséPascual Bravo, AlbertoDopamineHaemoglobinOxidative stressSympahtoadrenal lineageTyrosine hydroxylase23 páginas, 7 figuras, 2 tablas.Discovery of haemoglobin A expression outside of the erythroid cell lineage suggests that oxygen transport is the main, but not the unique, function of adult haemoglobin chains in mammals. The contribution of haemoglobin A to antioxidant defences has been proposed in the territories where it is expressed. Catecholaminergic cells rely on an active oxidative metabolism to accomplish their biological function, but are exposed to strong oxidative stress due to metabolism of catecholaminergic transmitters. We show in the present study that peripheral catecholaminegic cells express the α- and not the β-haemoglobin A chains, and that α-haemoglobin expression could modulate the antioxidant capabilities of these cells. We also show that α-haemoglobin overexpression in PC12 cells leads to a selective increase of tyrosine hydroxylase synthesis and activity. This is achieved by means of a reorganization of antioxidant defences, decreasing cytoplasmic glutathione peroxidase and superoxide dismutase, and increasing mitochondrial peroxidase. Moreover, α-haemoglobin induces a decrease in lipogenesis and increase in lipid degradation, situations that help save NAD(P)H and favour supply of acetyl-CoA to the tricarboxylic acid cycle and production of reducing equivalents in the cell. All of these results point to a role for α-haemoglobin as a regulator of catecholaminergic cell metabolism required for phenotype acquisition and maintenance.The TH-EGFP mice were obtained from the Gene Expression Nervous System Atlas (GENSAT) Project [NINDS contracts N01NS02331 and HHSN271200723701C to The Rockefeller University (New York, NY, U.S.A.)]. The work was funded by the Marcelino Botín Foundation, the Spanish Ministry of Science and Education, and the Andalusian Government. CIBERNED is funded by the Instituto de Salud Carlos III. Support from the Spanish Ministry of Science and Education for M.T.M.A. (“FPI” predoctoral fellowship) is also acknowledged.Peer reviewedBiochemical Society201220122011info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/49170reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1042/BJ20111640info:eu-repo/semantics/openAccessoai:digital.csic.es:10261/491702026-05-22T06:33:51Z
dc.title.none.fl_str_mv α-Haemoglobin regulates sympathoadrenal cell metabolism to maintain a catecholaminergic phenotype
title α-Haemoglobin regulates sympathoadrenal cell metabolism to maintain a catecholaminergic phenotype
spellingShingle α-Haemoglobin regulates sympathoadrenal cell metabolism to maintain a catecholaminergic phenotype
Marcos-Almaraz, María T.
Dopamine
Haemoglobin
Oxidative stress
Sympahtoadrenal lineage
Tyrosine hydroxylase
title_short α-Haemoglobin regulates sympathoadrenal cell metabolism to maintain a catecholaminergic phenotype
title_full α-Haemoglobin regulates sympathoadrenal cell metabolism to maintain a catecholaminergic phenotype
title_fullStr α-Haemoglobin regulates sympathoadrenal cell metabolism to maintain a catecholaminergic phenotype
title_full_unstemmed α-Haemoglobin regulates sympathoadrenal cell metabolism to maintain a catecholaminergic phenotype
title_sort α-Haemoglobin regulates sympathoadrenal cell metabolism to maintain a catecholaminergic phenotype
dc.creator.none.fl_str_mv Marcos-Almaraz, María T.
Rodríguez-Gómez, José A.
López-Barneo, José
Pascual Bravo, Alberto
author Marcos-Almaraz, María T.
author_facet Marcos-Almaraz, María T.
Rodríguez-Gómez, José A.
López-Barneo, José
Pascual Bravo, Alberto
author_role author
author2 Rodríguez-Gómez, José A.
López-Barneo, José
Pascual Bravo, Alberto
author2_role author
author
author
dc.subject.none.fl_str_mv Dopamine
Haemoglobin
Oxidative stress
Sympahtoadrenal lineage
Tyrosine hydroxylase
topic Dopamine
Haemoglobin
Oxidative stress
Sympahtoadrenal lineage
Tyrosine hydroxylase
description 23 páginas, 7 figuras, 2 tablas.
publishDate 2011
dc.date.none.fl_str_mv 2011
2012
2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/49170
url http://hdl.handle.net/10261/49170
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1042/BJ20111640
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Biochemical Society
publisher.none.fl_str_mv Biochemical Society
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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