Seminal plasma as an extender for short-term storage and cryopreservation of sperm in European sea bass

[EN] The preservation of male sperm is a tool gaining importance for assisted reproduction programs, genetic conservation, and reproductive management in aquaculture. This study compared the effects of natural seminal plasma and a synthetic non-activating medium (NAM) on the short-term storage and c...

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Autores: França, Thales Souza, Borges, L.P., Felip, A., Fernández-García, Fátima|||0009-0001-5070-3762, Asturiano, Juan F.|||0000-0002-6441-5294
Tipo de recurso: artículo
Fecha de publicación:2026
País:España
Institución:Universitat Politècnica de València (UPV)
Repositorio:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:inglés
OAI Identifier:oai:dnet:riunet______::742d83f532e598fe027f4d50556be64b
Acceso en línea:https://riunet.upv.es/handle/10251/235419
Access Level:acceso abierto
Palabra clave:CASA system
Sperm extenders
Sperm motility
Teleost
Cryobiology
Dicentrarchus labrax
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dc.title.none.fl_str_mv Seminal plasma as an extender for short-term storage and cryopreservation of sperm in European sea bass
title Seminal plasma as an extender for short-term storage and cryopreservation of sperm in European sea bass
spellingShingle Seminal plasma as an extender for short-term storage and cryopreservation of sperm in European sea bass
França, Thales Souza
CASA system
Sperm extenders
Sperm motility
Teleost
Cryobiology
Dicentrarchus labrax
title_short Seminal plasma as an extender for short-term storage and cryopreservation of sperm in European sea bass
title_full Seminal plasma as an extender for short-term storage and cryopreservation of sperm in European sea bass
title_fullStr Seminal plasma as an extender for short-term storage and cryopreservation of sperm in European sea bass
title_full_unstemmed Seminal plasma as an extender for short-term storage and cryopreservation of sperm in European sea bass
title_sort Seminal plasma as an extender for short-term storage and cryopreservation of sperm in European sea bass
dc.creator.none.fl_str_mv França, Thales Souza
Borges, L.P.
Felip, A.
Fernández-García, Fátima|||0009-0001-5070-3762
Asturiano, Juan F.|||0000-0002-6441-5294
author França, Thales Souza
author_facet França, Thales Souza
Borges, L.P.
Felip, A.
Fernández-García, Fátima|||0009-0001-5070-3762
Asturiano, Juan F.|||0000-0002-6441-5294
author_role author
author2 Borges, L.P.
Felip, A.
Fernández-García, Fátima|||0009-0001-5070-3762
Asturiano, Juan F.|||0000-0002-6441-5294
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Departamento de Ciencia Animal
Instituto Universitario de Ciencia y Tecnología Animal
Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural
European Commission
Generalitat Valenciana
Fundação de Amparo à Pesquisa do Estado de São Paulo
Conselho Nacional de Desenvolvimento Científico e Tecnológico, Brasil
Repositorio Institucional de la Universitat Politècnica de València Riunet
dc.subject.none.fl_str_mv CASA system
Sperm extenders
Sperm motility
Teleost
Cryobiology
Dicentrarchus labrax
topic CASA system
Sperm extenders
Sperm motility
Teleost
Cryobiology
Dicentrarchus labrax
description [EN] The preservation of male sperm is a tool gaining importance for assisted reproduction programs, genetic conservation, and reproductive management in aquaculture. This study compared the effects of natural seminal plasma and a synthetic non-activating medium (NAM) on the short-term storage and cryopreservation of sperm samples in European sea bass (Dicentrarchus labrax). The NAM extender consisted of (in mM): NaCl 59.83, KCl 1.47, MgCl2 12.91, CaCl2 3.51, NaHCO3 20, and glucose 0.44, supplemented with BSA (1% w/v), with an osmolality of 310 mOsm/kg and pH adjusted to 7.7. Two experiments were conducted: (1) sperm undergone refrigerated (4 degrees C) for up to 144 h, assessing total motility (MOT), progressive motility (MOTp), and additional kinetic parameters using a CASA system; (2) sperm was cryopreserved using DMSO followed by thawing, with evaluation of the same parameters and DNA integrity using the comet assay. In short-term storage, samples diluted with seminal plasma maintained significantly higher motility and kinetic parameters than those diluted with NAM up to 48 h, with a marked decline in both extenders after 144 h. In the cryopreservation trials, seminal plasma yielded better results for sperm motility and kinetic parameters, but no significant differences were found in DNA fragmentation between extenders. The findings indicate that natural seminal plasma, due to its biochemical composition rich in ions, proteins, and antioxidants, offers advantages in maintaining sperm motility and structural integrity compared with NAM. Nevertheless, the motility decline after prolonged storage underscores the need for optimized cryopreservation protocols. It is concluded that seminal plasma is a promising alternative to NAM for D. labrax sperm preservation, and further studies are recommended to standardize its concentration, supplement it with antioxidants, and assess post-thaw fertility.
publishDate 2026
dc.date.none.fl_str_mv 2026
2026-06-15
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://riunet.upv.es/handle/10251/235419
url https://riunet.upv.es/handle/10251/235419
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Conselho Nacional de Desenvolvimento Científico e Tecnológico, Brasil https://doi.org/10.13039/501100003593 88887.660920%2F2022 00
Conselho Nacional de Desenvolvimento Científico e Tecnológico, Brasil https://doi.org/10.13039/501100003593 200285%2F2021 1
Conselho Nacional de Desenvolvimento Científico e Tecnológico, Brasil https://doi.org/10.13039/501100003593 2518796
Fundação de Amparo à Pesquisa do Estado de São Paulo https://doi.org/10.13039/501100001807 2020%2F15020 0
Generalitat Valenciana https://doi.org/10.13039/501100003359 THINKINAZUL%2F2021%2F012
Generalitat Valenciana https://doi.org/10.13039/501100003359 THINKINAZUL%2F2021%2F042
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento (by)
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento (by)
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname:Universitat Politècnica de València (UPV)
instname_str Universitat Politècnica de València (UPV)
reponame_str RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
collection RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
repository.name.fl_str_mv
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spelling Seminal plasma as an extender for short-term storage and cryopreservation of sperm in European sea bassFrança, Thales SouzaBorges, L.P.Felip, A.Fernández-García, Fátima|||0009-0001-5070-3762Asturiano, Juan F.|||0000-0002-6441-5294CASA systemSperm extendersSperm motilityTeleostCryobiologyDicentrarchus labrax[EN] The preservation of male sperm is a tool gaining importance for assisted reproduction programs, genetic conservation, and reproductive management in aquaculture. This study compared the effects of natural seminal plasma and a synthetic non-activating medium (NAM) on the short-term storage and cryopreservation of sperm samples in European sea bass (Dicentrarchus labrax). The NAM extender consisted of (in mM): NaCl 59.83, KCl 1.47, MgCl2 12.91, CaCl2 3.51, NaHCO3 20, and glucose 0.44, supplemented with BSA (1% w/v), with an osmolality of 310 mOsm/kg and pH adjusted to 7.7. Two experiments were conducted: (1) sperm undergone refrigerated (4 degrees C) for up to 144 h, assessing total motility (MOT), progressive motility (MOTp), and additional kinetic parameters using a CASA system; (2) sperm was cryopreserved using DMSO followed by thawing, with evaluation of the same parameters and DNA integrity using the comet assay. In short-term storage, samples diluted with seminal plasma maintained significantly higher motility and kinetic parameters than those diluted with NAM up to 48 h, with a marked decline in both extenders after 144 h. In the cryopreservation trials, seminal plasma yielded better results for sperm motility and kinetic parameters, but no significant differences were found in DNA fragmentation between extenders. The findings indicate that natural seminal plasma, due to its biochemical composition rich in ions, proteins, and antioxidants, offers advantages in maintaining sperm motility and structural integrity compared with NAM. Nevertheless, the motility decline after prolonged storage underscores the need for optimized cryopreservation protocols. It is concluded that seminal plasma is a promising alternative to NAM for D. labrax sperm preservation, and further studies are recommended to standardize its concentration, supplement it with antioxidants, and assess post-thaw fertility.This study forms part of the ThinkInAzul programme and was sup-ported by MICIU with funding from European Union NextGenerationEU (PRTR-C17.I1) and by Generalitat Valenciana (GVA-THINKINAZUL/2021/012; Principal investigator: J.F. Asturiano, Universitat Politecnica de Valencia; and GVA-THINKINAZUL/2021/042; Principal investigator: A. Go mez, Spanish National Research Council) . LPB received a Sand-wich Doctorate fellowship from the Brazilian National Council for Sci-entific and Technological Development (CNPq; Proc. No. 88887.660920/2022-00) and acknowledges the financial support from the Sao Paulo Research Foundation (FAPESP; Proc. No. 2020/15020-0) and the Brazilian Coordination for the Improvement of Higher Educa-tion Personnel (CAPES) , through the Programa de Excele ncia Acade mica - PROEX (Proc. No. 88887.660920/2022-00) . TSF have fellowships from CNPq (200285/2021-1 and 2518796) .ElsevierDepartamento de Ciencia AnimalInstituto Universitario de Ciencia y Tecnología AnimalEscuela Técnica Superior de Ingeniería Agronómica y del Medio NaturalEuropean CommissionGeneralitat ValencianaFundação de Amparo à Pesquisa do Estado de São PauloConselho Nacional de Desenvolvimento Científico e Tecnológico, BrasilRepositorio Institucional de la Universitat Politècnica de València Riunet20262026-06-15journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://riunet.upv.es/handle/10251/235419reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengConselho Nacional de Desenvolvimento Científico e Tecnológico, Brasil https://doi.org/10.13039/501100003593 88887.660920%2F2022 00Conselho Nacional de Desenvolvimento Científico e Tecnológico, Brasil https://doi.org/10.13039/501100003593 200285%2F2021 1Conselho Nacional de Desenvolvimento Científico e Tecnológico, Brasil https://doi.org/10.13039/501100003593 2518796Fundação de Amparo à Pesquisa do Estado de São Paulo https://doi.org/10.13039/501100001807 2020%2F15020 0Generalitat Valenciana https://doi.org/10.13039/501100003359 THINKINAZUL%2F2021%2F012Generalitat Valenciana https://doi.org/10.13039/501100003359 THINKINAZUL%2F2021%2F042open accesshttp://purl.org/coar/access_right/c_abf2Reconocimiento (by)http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:dnet:riunet______::742d83f532e598fe027f4d50556be64b2026-06-13T07:49:27Z
score 15.812429

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