Comparing nucleic acid lateral flow and electrochemical genosensing for the simultaneous detection of foodborne pathogens

Due to the increasing need of rapid tests for application in low resource settings, WHO summarized their ideal features under the acronym ASSURED (Affordable, Sensitive, Specific, User-friendly, Rapid and Robust, Equipment-free, Delivered to those who need it). In this work, two different platforms...

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Autores: Ben Aissa Soler, Alejandra|||0000-0002-0419-0572, Jara, José Juan, Sebastián, Rosa María|||0000-0001-5519-9131, Vallribera, Adelina|||0000-0002-6452-4589, Campoy Sánchez, Susana|||0000-0003-1369-0197, Pividori, María Isabel|||0000-0002-5266-7873
Tipo de recurso: artículo
Fecha de publicación:2017
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:273775
Acceso en línea:https://ddd.uab.cat/record/273775
https://dx.doi.org/urn:doi:10.1016/j.bios.2016.08.046
Access Level:acceso abierto
Palabra clave:Nucleic acid lateral flow
Electrochemical magneto genosensing
Foodborne bacteria
Simultaneous detection
Magnetic particles
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spelling Comparing nucleic acid lateral flow and electrochemical genosensing for the simultaneous detection of foodborne pathogensBen Aissa Soler, Alejandra|||0000-0002-0419-0572Jara, José JuanSebastián, Rosa María|||0000-0001-5519-9131Vallribera, Adelina|||0000-0002-6452-4589Campoy Sánchez, Susana|||0000-0003-1369-0197Pividori, María Isabel|||0000-0002-5266-7873Nucleic acid lateral flowElectrochemical magneto genosensingFoodborne bacteriaSimultaneous detectionMagnetic particlesDue to the increasing need of rapid tests for application in low resource settings, WHO summarized their ideal features under the acronym ASSURED (Affordable, Sensitive, Specific, User-friendly, Rapid and Robust, Equipment-free, Delivered to those who need it). In this work, two different platforms for the rapid and simultaneous testing of the foodborne pathogens E. coli O157:H7 and Salmonella enterica, in detail a nucleic acid lateral flow and an electrochemical magneto-genosensor are presented and compared in terms of their analytical performance. The DNA of the bacteria was amplified by polymerase chain reaction using a quadruple-tagging set of primers specific for E. coli eaeA (151 bp) and Salmonella enterica yfiR (375 bp) genes. During the amplification, the amplicons were labelled at the same time with biotin/digoxigenin or biotin/fluorescein tags, respectively. The nucleic acid lateral flow assay was based on the use of streptavidin gold nanoparticles for the labelling of the tagged amplicon from E. coli and Salmonella. The visual readout was achieved when the gold-modified amplicons were captured by the specific antibodies. The features of this approach are discussed and compared with an electrochemical magneto-genosensor. Although nucleic acid lateral flow showed higher limit of detection, this strategy was able to clearly distinguish positive and negative samples of both bacteria being considered as a rapid and promising detection tool for bacteria screening. 22017-01-0120172017-01-01Articlehttp://purl.org/coar/resource_type/c_6501AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/273775https://dx.doi.org/urn:doi:10.1016/j.bios.2016.08.046reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengMinisterio de Economía y Competitividad https://doi.org/10.13039/501100003329 BIO2013-41242-RMinisterio de Economía y Competitividad https://doi.org/10.13039/501100003329 BFU2011-23478Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 CTQ2014-53662-PMinisterio de Economía y Competitividad https://doi.org/10.13039/501100003329 CTQ2014-51912-REDCAgència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2014/SGR-837Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2014/SGR-572Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2014/SGR-1105open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.https://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2737752026-06-06T12:50:31Z
dc.title.none.fl_str_mv Comparing nucleic acid lateral flow and electrochemical genosensing for the simultaneous detection of foodborne pathogens
title Comparing nucleic acid lateral flow and electrochemical genosensing for the simultaneous detection of foodborne pathogens
spellingShingle Comparing nucleic acid lateral flow and electrochemical genosensing for the simultaneous detection of foodborne pathogens
Ben Aissa Soler, Alejandra|||0000-0002-0419-0572
Nucleic acid lateral flow
Electrochemical magneto genosensing
Foodborne bacteria
Simultaneous detection
Magnetic particles
title_short Comparing nucleic acid lateral flow and electrochemical genosensing for the simultaneous detection of foodborne pathogens
title_full Comparing nucleic acid lateral flow and electrochemical genosensing for the simultaneous detection of foodborne pathogens
title_fullStr Comparing nucleic acid lateral flow and electrochemical genosensing for the simultaneous detection of foodborne pathogens
title_full_unstemmed Comparing nucleic acid lateral flow and electrochemical genosensing for the simultaneous detection of foodborne pathogens
title_sort Comparing nucleic acid lateral flow and electrochemical genosensing for the simultaneous detection of foodborne pathogens
dc.creator.none.fl_str_mv Ben Aissa Soler, Alejandra|||0000-0002-0419-0572
Jara, José Juan
Sebastián, Rosa María|||0000-0001-5519-9131
Vallribera, Adelina|||0000-0002-6452-4589
Campoy Sánchez, Susana|||0000-0003-1369-0197
Pividori, María Isabel|||0000-0002-5266-7873
author Ben Aissa Soler, Alejandra|||0000-0002-0419-0572
author_facet Ben Aissa Soler, Alejandra|||0000-0002-0419-0572
Jara, José Juan
Sebastián, Rosa María|||0000-0001-5519-9131
Vallribera, Adelina|||0000-0002-6452-4589
Campoy Sánchez, Susana|||0000-0003-1369-0197
Pividori, María Isabel|||0000-0002-5266-7873
author_role author
author2 Jara, José Juan
Sebastián, Rosa María|||0000-0001-5519-9131
Vallribera, Adelina|||0000-0002-6452-4589
Campoy Sánchez, Susana|||0000-0003-1369-0197
Pividori, María Isabel|||0000-0002-5266-7873
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Nucleic acid lateral flow
Electrochemical magneto genosensing
Foodborne bacteria
Simultaneous detection
Magnetic particles
topic Nucleic acid lateral flow
Electrochemical magneto genosensing
Foodborne bacteria
Simultaneous detection
Magnetic particles
description Due to the increasing need of rapid tests for application in low resource settings, WHO summarized their ideal features under the acronym ASSURED (Affordable, Sensitive, Specific, User-friendly, Rapid and Robust, Equipment-free, Delivered to those who need it). In this work, two different platforms for the rapid and simultaneous testing of the foodborne pathogens E. coli O157:H7 and Salmonella enterica, in detail a nucleic acid lateral flow and an electrochemical magneto-genosensor are presented and compared in terms of their analytical performance. The DNA of the bacteria was amplified by polymerase chain reaction using a quadruple-tagging set of primers specific for E. coli eaeA (151 bp) and Salmonella enterica yfiR (375 bp) genes. During the amplification, the amplicons were labelled at the same time with biotin/digoxigenin or biotin/fluorescein tags, respectively. The nucleic acid lateral flow assay was based on the use of streptavidin gold nanoparticles for the labelling of the tagged amplicon from E. coli and Salmonella. The visual readout was achieved when the gold-modified amplicons were captured by the specific antibodies. The features of this approach are discussed and compared with an electrochemical magneto-genosensor. Although nucleic acid lateral flow showed higher limit of detection, this strategy was able to clearly distinguish positive and negative samples of both bacteria being considered as a rapid and promising detection tool for bacteria screening.
publishDate 2017
dc.date.none.fl_str_mv 2
2017-01-01
2017
2017-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
AM
http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/273775
https://dx.doi.org/urn:doi:10.1016/j.bios.2016.08.046
url https://ddd.uab.cat/record/273775
https://dx.doi.org/urn:doi:10.1016/j.bios.2016.08.046
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 BIO2013-41242-R
Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 BFU2011-23478
Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 CTQ2014-53662-P
Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 CTQ2014-51912-REDC
Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2014/SGR-837
Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2014/SGR-572
Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2014/SGR-1105
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
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