A Hyaluronic Acid Demilune Scaffold and Polypyrrole-Coated Fibers Carrying Embedded Human Neural Precursor Cells and Curcumin for Surface Capping of Spinal Cord Injuries

[EN] Tissue engineering, including cell transplantation and the application of biomaterials and bioactive molecules, represents a promising approach for regeneration following spinal cord injury (SCI). We designed a combinatorial tissue-engineered approach for the minimally invasive treatment of SCI...

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Autores: Elkhenany, Hoda, Bonilla, Pablo, Alastrue Agudo, Ana, Edel, Michael J., Vicent, María Jesus, Rodriguez Doblado, Laura, Moreno Manzano, Victoria, Giraldo, Esther|||0000-0001-5488-3011, Gisbert-Roca, Fernando|||0000-0003-3937-5822, Martínez-Ramos, Cristina|||0000-0002-6540-4714, Monleón Pradas, Manuel|||0000-0001-6457-0414
Tipo de recurso: artículo
Fecha de publicación:2021
País:España
Institución:Universitat Politècnica de València (UPV)
Repositorio:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:inglés
OAI Identifier:oai:riunet.upv.es:10251/181544
Acceso en línea:https://riunet.upv.es/handle/10251/181544
Access Level:acceso abierto
Palabra clave:Spinal cord injury
PuraMatrix hydrogel
Hyaluronic acid
Biomaterials
Induced neural progenitor cells
Curcumin
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network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv A Hyaluronic Acid Demilune Scaffold and Polypyrrole-Coated Fibers Carrying Embedded Human Neural Precursor Cells and Curcumin for Surface Capping of Spinal Cord Injuries
title A Hyaluronic Acid Demilune Scaffold and Polypyrrole-Coated Fibers Carrying Embedded Human Neural Precursor Cells and Curcumin for Surface Capping of Spinal Cord Injuries
spellingShingle A Hyaluronic Acid Demilune Scaffold and Polypyrrole-Coated Fibers Carrying Embedded Human Neural Precursor Cells and Curcumin for Surface Capping of Spinal Cord Injuries
Elkhenany, Hoda
Spinal cord injury
PuraMatrix hydrogel
Hyaluronic acid
Biomaterials
Induced neural progenitor cells
Curcumin
title_short A Hyaluronic Acid Demilune Scaffold and Polypyrrole-Coated Fibers Carrying Embedded Human Neural Precursor Cells and Curcumin for Surface Capping of Spinal Cord Injuries
title_full A Hyaluronic Acid Demilune Scaffold and Polypyrrole-Coated Fibers Carrying Embedded Human Neural Precursor Cells and Curcumin for Surface Capping of Spinal Cord Injuries
title_fullStr A Hyaluronic Acid Demilune Scaffold and Polypyrrole-Coated Fibers Carrying Embedded Human Neural Precursor Cells and Curcumin for Surface Capping of Spinal Cord Injuries
title_full_unstemmed A Hyaluronic Acid Demilune Scaffold and Polypyrrole-Coated Fibers Carrying Embedded Human Neural Precursor Cells and Curcumin for Surface Capping of Spinal Cord Injuries
title_sort A Hyaluronic Acid Demilune Scaffold and Polypyrrole-Coated Fibers Carrying Embedded Human Neural Precursor Cells and Curcumin for Surface Capping of Spinal Cord Injuries
dc.creator.none.fl_str_mv Elkhenany, Hoda
Bonilla, Pablo
Alastrue Agudo, Ana
Edel, Michael J.
Vicent, María Jesus
Rodriguez Doblado, Laura
Moreno Manzano, Victoria
Giraldo, Esther|||0000-0001-5488-3011
Gisbert-Roca, Fernando|||0000-0003-3937-5822
Martínez-Ramos, Cristina|||0000-0002-6540-4714
Monleón Pradas, Manuel|||0000-0001-6457-0414
author Elkhenany, Hoda
author_facet Elkhenany, Hoda
Bonilla, Pablo
Alastrue Agudo, Ana
Edel, Michael J.
Vicent, María Jesus
Rodriguez Doblado, Laura
Moreno Manzano, Victoria
Giraldo, Esther|||0000-0001-5488-3011
Gisbert-Roca, Fernando|||0000-0003-3937-5822
Martínez-Ramos, Cristina|||0000-0002-6540-4714
Monleón Pradas, Manuel|||0000-0001-6457-0414
author_role author
author2 Bonilla, Pablo
Alastrue Agudo, Ana
Edel, Michael J.
Vicent, María Jesus
Rodriguez Doblado, Laura
Moreno Manzano, Victoria
Giraldo, Esther|||0000-0001-5488-3011
Gisbert-Roca, Fernando|||0000-0003-3937-5822
Martínez-Ramos, Cristina|||0000-0002-6540-4714
Monleón Pradas, Manuel|||0000-0001-6457-0414
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Departamento de Física Aplicada
Departamento de Biotecnología
Departamento de Termodinámica Aplicada
Centro de Biomateriales e Ingeniería Tisular
Escuela Técnica Superior de Ingeniería Industrial
Centro Avanzado de Microbiología Aplicada
Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural
European Commission
Generalitat Valenciana
AGENCIA ESTATAL DE INVESTIGACION
European Regional Development Fund
Ministerio de Ciencia e Innovación
Repositorio Institucional de la Universitat Politècnica de València Riunet
dc.subject.none.fl_str_mv Spinal cord injury
PuraMatrix hydrogel
Hyaluronic acid
Biomaterials
Induced neural progenitor cells
Curcumin
topic Spinal cord injury
PuraMatrix hydrogel
Hyaluronic acid
Biomaterials
Induced neural progenitor cells
Curcumin
description [EN] Tissue engineering, including cell transplantation and the application of biomaterials and bioactive molecules, represents a promising approach for regeneration following spinal cord injury (SCI). We designed a combinatorial tissue-engineered approach for the minimally invasive treatment of SCI¿a hyaluronic acid (HA)-based scaffold containing polypyrrole-coated fibers (PPY) combined with the RAD16-I self-assembling peptide hydrogel (Corning® PuraMatrix¿peptide hydrogel (PM)), human induced neural progenitor cells (iNPCs), and a nanoconjugated form of curcumin (CURC). In vitro cultures demonstrated that PM preserves iNPC viability and the addition of CURC reduces apoptosis and enhances the outgrowth of Nestin-positive neurites from iNPCs, compared to nonembedded iNPCs. The treatment of spinal cord organotypic cultures also demonstrated that CURC enhances cell migration and prompts a neuron-like morphology of embedded iNPCs implanted over the tissue slices. Following sub-acute SCI by traumatic contusion in rats, the implantation of PMembedded iNPCs and CURC with PPY fibers supported a significant increase in neuro-preservation (as measured by greater III-tubulin staining of neuronal fibers) and decrease in the injured area (as measured by the lack of GFAP staining). This combination therapy also restricted platelet-derived growth factor expression, indicating a reduction in fibrotic pericyte invasion. Overall, these findings support PM-embedded iNPCs with CURC placed within an HA demilune scaffold containing PPY fibers as a minimally invasive combination-based alternative to cell transplantation alone.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021-12-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://riunet.upv.es/handle/10251/181544
url https://riunet.upv.es/handle/10251/181544
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 RTI2018-095872-B-C21 NUEVO BIOMATERIAL BIO-ACTIVO PARA LA REGENERACION DE LESIONES MEDULARES
European Commission https://doi.org/10.13039/501100000780 H2020-FETOPEN-2018-2019-2020-01
Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 RTI2018-095872-B-C22 NUEVO DISPOSITIVO BIOACTIVO PARA LA REGENERACION DE LESIONES DE LA MEDULA ESPINAL
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento (by)
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento (by)
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI AG
publisher.none.fl_str_mv MDPI AG
dc.source.none.fl_str_mv reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname:Universitat Politècnica de València (UPV)
instname_str Universitat Politècnica de València (UPV)
reponame_str RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
collection RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
repository.name.fl_str_mv
repository.mail.fl_str_mv
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spelling A Hyaluronic Acid Demilune Scaffold and Polypyrrole-Coated Fibers Carrying Embedded Human Neural Precursor Cells and Curcumin for Surface Capping of Spinal Cord InjuriesElkhenany, HodaBonilla, PabloAlastrue Agudo, AnaEdel, Michael J.Vicent, María JesusRodriguez Doblado, LauraMoreno Manzano, VictoriaGiraldo, Esther|||0000-0001-5488-3011Gisbert-Roca, Fernando|||0000-0003-3937-5822Martínez-Ramos, Cristina|||0000-0002-6540-4714Monleón Pradas, Manuel|||0000-0001-6457-0414Spinal cord injuryPuraMatrix hydrogelHyaluronic acidBiomaterialsInduced neural progenitor cellsCurcumin[EN] Tissue engineering, including cell transplantation and the application of biomaterials and bioactive molecules, represents a promising approach for regeneration following spinal cord injury (SCI). We designed a combinatorial tissue-engineered approach for the minimally invasive treatment of SCI¿a hyaluronic acid (HA)-based scaffold containing polypyrrole-coated fibers (PPY) combined with the RAD16-I self-assembling peptide hydrogel (Corning® PuraMatrix¿peptide hydrogel (PM)), human induced neural progenitor cells (iNPCs), and a nanoconjugated form of curcumin (CURC). In vitro cultures demonstrated that PM preserves iNPC viability and the addition of CURC reduces apoptosis and enhances the outgrowth of Nestin-positive neurites from iNPCs, compared to nonembedded iNPCs. The treatment of spinal cord organotypic cultures also demonstrated that CURC enhances cell migration and prompts a neuron-like morphology of embedded iNPCs implanted over the tissue slices. Following sub-acute SCI by traumatic contusion in rats, the implantation of PMembedded iNPCs and CURC with PPY fibers supported a significant increase in neuro-preservation (as measured by greater III-tubulin staining of neuronal fibers) and decrease in the injured area (as measured by the lack of GFAP staining). This combination therapy also restricted platelet-derived growth factor expression, indicating a reduction in fibrotic pericyte invasion. Overall, these findings support PM-embedded iNPCs with CURC placed within an HA demilune scaffold containing PPY fibers as a minimally invasive combination-based alternative to cell transplantation alone.This research was funded by the Science by Women program, Women for Africa Foundation to H.E. and the grants FEDER/Ministerio de Ciencia e Innovacion-Agencia Estatal de Investigacion [RTI2018-095872-B-C21 and -C22/ERDF]; Part of the equipment employed in this work was funded by Generalitat Valenciana and cofinanced with ERDF funds (OP ERDF of Comunitat Valenciana 2014-2020). RISEUP project FetOpen in H2020 Program: H2020-FETOPEN-2018-2019-2020-01.MDPI AGDepartamento de Física AplicadaDepartamento de BiotecnologíaDepartamento de Termodinámica AplicadaCentro de Biomateriales e Ingeniería TisularEscuela Técnica Superior de Ingeniería IndustrialCentro Avanzado de Microbiología AplicadaEscuela Técnica Superior de Ingeniería Agronómica y del Medio NaturalEuropean CommissionGeneralitat ValencianaAGENCIA ESTATAL DE INVESTIGACIONEuropean Regional Development FundMinisterio de Ciencia e InnovaciónRepositorio Institucional de la Universitat Politècnica de València Riunet20212021-12-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://riunet.upv.es/handle/10251/181544reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengAgencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 RTI2018-095872-B-C21 NUEVO BIOMATERIAL BIO-ACTIVO PARA LA REGENERACION DE LESIONES MEDULARESEuropean Commission https://doi.org/10.13039/501100000780 H2020-FETOPEN-2018-2019-2020-01Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 RTI2018-095872-B-C22 NUEVO DISPOSITIVO BIOACTIVO PARA LA REGENERACION DE LESIONES DE LA MEDULA ESPINALopen accesshttp://purl.org/coar/access_right/c_abf2Reconocimiento (by)http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/1815442026-06-13T07:49:27Z
score 15,301603

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