Aberrant Epstein-Barr virus antibody patterns and chronic lymphocytic leukemia in a Spanish multicentric case-control study
Background: Epstein-Barr virus (EBV)-related malignancies harbour distinct serological responses to EBV antigens. We hypothesized that EBV serological patterns can be useful to identify different stages of chronic lymphocytic leukemia. Methods: Information on 150 cases with chronic lymphocytic leuke...
| Autores: | , , , , , , , , , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2015 |
| País: | España |
| Institución: | Universitat Pompeu Fabra |
| Repositorio: | Repositorio Digital de la UPF |
| OAI Identifier: | oai:repositori.upf.edu:10230/23825 |
| Acceso en línea: | http://hdl.handle.net/10230/23825 http://dx.doi.org/10.1186/1750-9378-10-5 |
| Access Level: | acceso abierto |
| Palabra clave: | Leucèmia limfocítica crònica Virus d&apos Epstein-Barr Chronic lymphocytic leukemia Epstein-Barr virus Serology Case-control Smoking |
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| dc.title.none.fl_str_mv |
Aberrant Epstein-Barr virus antibody patterns and chronic lymphocytic leukemia in a Spanish multicentric case-control study |
| title |
Aberrant Epstein-Barr virus antibody patterns and chronic lymphocytic leukemia in a Spanish multicentric case-control study |
| spellingShingle |
Aberrant Epstein-Barr virus antibody patterns and chronic lymphocytic leukemia in a Spanish multicentric case-control study Casabonne, Delphine Leucèmia limfocítica crònica Virus d&apos Epstein-Barr Chronic lymphocytic leukemia Epstein-Barr virus Serology Case-control Smoking |
| title_short |
Aberrant Epstein-Barr virus antibody patterns and chronic lymphocytic leukemia in a Spanish multicentric case-control study |
| title_full |
Aberrant Epstein-Barr virus antibody patterns and chronic lymphocytic leukemia in a Spanish multicentric case-control study |
| title_fullStr |
Aberrant Epstein-Barr virus antibody patterns and chronic lymphocytic leukemia in a Spanish multicentric case-control study |
| title_full_unstemmed |
Aberrant Epstein-Barr virus antibody patterns and chronic lymphocytic leukemia in a Spanish multicentric case-control study |
| title_sort |
Aberrant Epstein-Barr virus antibody patterns and chronic lymphocytic leukemia in a Spanish multicentric case-control study |
| dc.creator.none.fl_str_mv |
Casabonne, Delphine Benavente, Yolanda Robles Hellín, Claudia, 1980- Costas, Laura Alonso, Esther González, Eva Tardón, Adonina Dierssen Sotos, Trinidad Gimeno Vázquez, Eva Aymerich, Marta Campo, Elias Castaño Vinyals, Gemma Aragonés, Núria Pollán, Marina Kogevinas, Manolis Juwana, Hedy Middeldorp, Jaap de Sanjosé, Silvia |
| author |
Casabonne, Delphine |
| author_facet |
Casabonne, Delphine Benavente, Yolanda Robles Hellín, Claudia, 1980- Costas, Laura Alonso, Esther González, Eva Tardón, Adonina Dierssen Sotos, Trinidad Gimeno Vázquez, Eva Aymerich, Marta Campo, Elias Castaño Vinyals, Gemma Aragonés, Núria Pollán, Marina Kogevinas, Manolis Juwana, Hedy Middeldorp, Jaap de Sanjosé, Silvia |
| author_role |
author |
| author2 |
Benavente, Yolanda Robles Hellín, Claudia, 1980- Costas, Laura Alonso, Esther González, Eva Tardón, Adonina Dierssen Sotos, Trinidad Gimeno Vázquez, Eva Aymerich, Marta Campo, Elias Castaño Vinyals, Gemma Aragonés, Núria Pollán, Marina Kogevinas, Manolis Juwana, Hedy Middeldorp, Jaap de Sanjosé, Silvia |
| author2_role |
author author author author author author author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
Leucèmia limfocítica crònica Virus d&apos Epstein-Barr Chronic lymphocytic leukemia Epstein-Barr virus Serology Case-control Smoking |
| topic |
Leucèmia limfocítica crònica Virus d&apos Epstein-Barr Chronic lymphocytic leukemia Epstein-Barr virus Serology Case-control Smoking |
| description |
Background: Epstein-Barr virus (EBV)-related malignancies harbour distinct serological responses to EBV antigens. We hypothesized that EBV serological patterns can be useful to identify different stages of chronic lymphocytic leukemia. Methods: Information on 150 cases with chronic lymphocytic leukemia and 157 frequency-matched (by age, sex and region) population-based controls from a Spanish multicentre case-control study was obtained. EBV immunoglobulin G serostatus was evaluated through a peptide-based ELISA and further by immunoblot analysis to EBV early antigens (EA), nuclear antigen (EBNA1), VCA-p18, VCA-p40 and Zebra. Two independent individuals categorized the serological patterns of the western blot analysis. Patients with very high response and diversity in EBV-specific polypeptides, in particular with clear responses to EA-associated proteins, were categorized as having an abnormal reactive pattern (ab_EBV). Adjusted odds ratios (OR) and 95% confidence interval (CI) were estimated using logistic regression models. Results: Almost all subjects were EBV-IgG positive (>95% of cases and controls) whereas ab_EBV patterns were detected in 23% of cases (N = 34) and 11% of controls (N = 17; OR: 2.44, 95% CI, 1.29 to 4.62; P = 0.006), particularly in intermediate/high risk patients. Although based on small numbers, the association was modified by smoking with a gradual reduction of ab_EBV-related OR for all Rai stages from never smokers to current smokers. Conclusions: Highly distinct EBV antibody diversity patterns revealed by immunoblot analysis were detected in cases compared to controls, detectable at very early stages of the disease and particularly among non smokers. This study provides further evidence of an abnormal immunological response against EBV in patients with chronic lymphocytic leukemia. |
| publishDate |
2015 |
| dc.date.none.fl_str_mv |
2015 2015 2015 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
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article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/10230/23825 http://dx.doi.org/10.1186/1750-9378-10-5 |
| url |
http://hdl.handle.net/10230/23825 http://dx.doi.org/10.1186/1750-9378-10-5 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
Infectious Agents and Cancer. 2015;10:5 |
| dc.rights.none.fl_str_mv |
http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
http://creativecommons.org/licenses/by/4.0 |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf application/pdf |
| dc.publisher.none.fl_str_mv |
BioMed Central |
| publisher.none.fl_str_mv |
BioMed Central |
| dc.source.none.fl_str_mv |
reponame:Repositorio Digital de la UPF instname:Universitat Pompeu Fabra |
| instname_str |
Universitat Pompeu Fabra |
| reponame_str |
Repositorio Digital de la UPF |
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Repositorio Digital de la UPF |
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|
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|
| _version_ |
1869421531506933760 |
| spelling |
Aberrant Epstein-Barr virus antibody patterns and chronic lymphocytic leukemia in a Spanish multicentric case-control studyCasabonne, DelphineBenavente, YolandaRobles Hellín, Claudia, 1980-Costas, LauraAlonso, EstherGonzález, EvaTardón, AdoninaDierssen Sotos, TrinidadGimeno Vázquez, EvaAymerich, MartaCampo, EliasCastaño Vinyals, GemmaAragonés, NúriaPollán, MarinaKogevinas, ManolisJuwana, HedyMiddeldorp, Jaapde Sanjosé, SilviaLeucèmia limfocítica crònicaVirus d&aposEpstein-BarrChronic lymphocytic leukemiaEpstein-Barr virusSerologyCase-controlSmokingBackground: Epstein-Barr virus (EBV)-related malignancies harbour distinct serological responses to EBV antigens. We hypothesized that EBV serological patterns can be useful to identify different stages of chronic lymphocytic leukemia. Methods: Information on 150 cases with chronic lymphocytic leukemia and 157 frequency-matched (by age, sex and region) population-based controls from a Spanish multicentre case-control study was obtained. EBV immunoglobulin G serostatus was evaluated through a peptide-based ELISA and further by immunoblot analysis to EBV early antigens (EA), nuclear antigen (EBNA1), VCA-p18, VCA-p40 and Zebra. Two independent individuals categorized the serological patterns of the western blot analysis. Patients with very high response and diversity in EBV-specific polypeptides, in particular with clear responses to EA-associated proteins, were categorized as having an abnormal reactive pattern (ab_EBV). Adjusted odds ratios (OR) and 95% confidence interval (CI) were estimated using logistic regression models. Results: Almost all subjects were EBV-IgG positive (>95% of cases and controls) whereas ab_EBV patterns were detected in 23% of cases (N = 34) and 11% of controls (N = 17; OR: 2.44, 95% CI, 1.29 to 4.62; P = 0.006), particularly in intermediate/high risk patients. Although based on small numbers, the association was modified by smoking with a gradual reduction of ab_EBV-related OR for all Rai stages from never smokers to current smokers. Conclusions: Highly distinct EBV antibody diversity patterns revealed by immunoblot analysis were detected in cases compared to controls, detectable at very early stages of the disease and particularly among non smokers. This study provides further evidence of an abnormal immunological response against EBV in patients with chronic lymphocytic leukemia.This work was supported by the “Acción Transversal del Cancer”, approved on the Spanish Ministry Council on the 11th October 2007, by the Instituto de Salud Carlos III (Spanish Government) (grants PI08/1770, PI08/0533, PI08/1359, PS09/00773-Cantabria, PI11/01810, PI11/02213, RCESP C03/09, RTICESP C03/10, RTIC RD06/0020/0095, RTIC RD12/0036/0056, Rio Hortega CM13/00232, SV-09-CLINIC-1 and CIBERESP), by the Fundación Marques de Valdecilla (API 10/09), by Obra Social CAJASTUR (SV-CAJASTUR-1), by the Recercaixa (2010ACUP 00310), by the Spanish Association Against Cancer (AECC) Scientific Foundation and by the Agència de Gestió d’Ajuts Universitaris i de Recerca (AGAUR)–Generalitat de Catalunya (Catalonian Government) (grants AGAUR 2009SGR1026, 2014SGR756 and 2009SGR1465). The ICGC CLL-Genome Project is funded by Spanish Ministerio de Economía y Competitividad (MINECO) through the Instituto de Salud Carlos III (ISCIII) and Red Temática de Investigación del Cáncer (RTIC RD12/0036/0036) del ISCIII. Sample collection and storage was partially supported by the Instituto de Salud Carlos III FEDER (RD09/0076/00036), Xarxa de Bancs de Tumors de Catalunya sponsored by Pla Director d’Oncologia de Catalunya (XBTC).BioMed Central201520152015info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/23825http://dx.doi.org/10.1186/1750-9378-10-5reponame:Repositorio Digital de la UPFinstname:Universitat Pompeu FabraInglésInfectious Agents and Cancer. 2015;10:5© 2015 Casabonne et al.; licensee BioMed Central. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.http://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessoai:repositori.upf.edu:10230/238252026-06-12T07:21:37Z |
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15.198674 |