Oxidative Stress in Wild Boars Naturally and Experimentally Infected with Mycobacterium bovis

Reactive oxygen and nitrogen species (ROS-RNS) are important defence substances involved in the immune response against pathogens. An excessive increase in ROS-RNS, however, can damage the organism causing oxidative stress (OS). The organism is able to neutralise OS by the production of antioxidant...

Descripción completa

Detalles Bibliográficos
Autores: Gassó Garcia, Diana, Vicente, Joaquín, Mentaberre García, Gregorio, Soriguer, Ramón, Jiménez Rodríguez, Rocío, Navarro González, Nora, Tvarijonaviciute, Asta, Lavín, Santiago, Fernández-Llario, Pedroo, Segalés, Joaquim, Serrano, Emmanuel
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:España
Institución:Universitat de Lleida (UdL)
Repositorio:Repositori Obert UdL
OAI Identifier:oai:repositori.udl.cat:10459.1/464920
Acceso en línea:https://doi.org/10.1371/journal.pone.0163971
https://hdl.handle.net/10459.1/464920
Access Level:acceso abierto
id ES_da349ede4c76eebbe33ed783073fcf2c
oai_identifier_str oai:repositori.udl.cat:10459.1/464920
network_acronym_str ES
network_name_str España
repository_id_str
spelling Oxidative Stress in Wild Boars Naturally and Experimentally Infected with Mycobacterium bovisGassó Garcia, DianaVicente, JoaquínMentaberre García, GregorioSoriguer, RamónJiménez Rodríguez, RocíoNavarro González, NoraTvarijonaviciute, AstaLavín, SantiagoFernández-Llario, PedrooSegalés, JoaquimSerrano, EmmanuelReactive oxygen and nitrogen species (ROS-RNS) are important defence substances involved in the immune response against pathogens. An excessive increase in ROS-RNS, however, can damage the organism causing oxidative stress (OS). The organism is able to neutralise OS by the production of antioxidant enzymes (AE); hence, tissue damage is the result of an imbalance between oxidant and antioxidant status. Though some work has been carried out in humans, there is a lack of information about the oxidant/antioxidant status in the presence of tuberculosis (TB) in wild reservoirs. In the Mediterranean Basin, wild boar (Sus scrofa) is the main reservoir of TB. Wild boar showing severe TB have an increased risk to Mycobacterium spp. shedding, leading to pathogen spreading and persistence. If OS is greater in these individuals, oxidant/antioxidant balance in TB-affected boars could be used as a biomarker of disease severity. The present work had a two-fold objective: i) to study the effects of bovine TB on different OS biomarkers (namely superoxide dismutase (SOD), catalasa (CAT), glutathione peroxidase (GPX), glutathione reductase (GR) and thiobarbituric acid reactive substances (TBARS)) in wild boar experimentally challenged with Mycobacterium bovis, and ii) to explore the role of body weight, sex, population and season in explaining the observed variability of OS indicators in two populations of free-ranging wild boar where TB is common. For the first objective, a partial least squares regression (PLSR) approach was used whereas, recursive partitioning with regression tree models (RTM) were applied for the second. A negative relationship between antioxidant enzymes and bovine TB (the more severe lesions, the lower the concentration of antioxidant biomarkers) was observed in experimentally infected animals. The final PLSR model permits retained the GPX, SOD and GR biomarkers and showed that 17.6% of the observed variability of antioxidant capacity was significantly correlated with the PLSR X's component represented by both disease status and the age of boars. In the samples from free-ranging wild boar, however, the environmental factors were more relevant to the observed variability of the OS biomarkers than the TB itself. For each OS biomarker, each RTM was defined as a maximum by one node due to the population effect. Along the same lines, the ad hoc tree regression on boars from the population with a higher prevalence of severe TB confirmed that disease status was not the main factor explaining the observed variability in OS biomarkers. It was concluded that oxidative damage caused by TB is significant, but can only be detected in the absence of environmental variation in wild boar.This work was funded by the PAIDI Research Group RNM18 from Junta de Andalucía. E. Serrano was supported by the postdoctoral program (SFRH/BPD/96637/2013) of the Fundação para a Ciência ea Tecnologia, Portugal. We would like to thank the University of Aveiro (Department of Biology) and FCT/MEC for the financial support to CESAM RU (UID/AMB/50017) through national funds and, where applicable, co-financed by the FEDER, within the PT2020 Partnership Agreement.Public Library of Science2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://doi.org/10.1371/journal.pone.0163971https://hdl.handle.net/10459.1/464920reponame:Repositori Obert UdL instname:Universitat de Lleida (UdL)InglésReproducció del document publicat a: https://doi.org/10.1371/journal.pone.0163971Plos One, 2016, vol. 11, núm. 9, p. e0163971cc-by (c) Gassó et al., 2016info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/4.0/oai:repositori.udl.cat:10459.1/4649202026-06-24T12:42:17Z
dc.title.none.fl_str_mv Oxidative Stress in Wild Boars Naturally and Experimentally Infected with Mycobacterium bovis
title Oxidative Stress in Wild Boars Naturally and Experimentally Infected with Mycobacterium bovis
spellingShingle Oxidative Stress in Wild Boars Naturally and Experimentally Infected with Mycobacterium bovis
Gassó Garcia, Diana
title_short Oxidative Stress in Wild Boars Naturally and Experimentally Infected with Mycobacterium bovis
title_full Oxidative Stress in Wild Boars Naturally and Experimentally Infected with Mycobacterium bovis
title_fullStr Oxidative Stress in Wild Boars Naturally and Experimentally Infected with Mycobacterium bovis
title_full_unstemmed Oxidative Stress in Wild Boars Naturally and Experimentally Infected with Mycobacterium bovis
title_sort Oxidative Stress in Wild Boars Naturally and Experimentally Infected with Mycobacterium bovis
dc.creator.none.fl_str_mv Gassó Garcia, Diana
Vicente, Joaquín
Mentaberre García, Gregorio
Soriguer, Ramón
Jiménez Rodríguez, Rocío
Navarro González, Nora
Tvarijonaviciute, Asta
Lavín, Santiago
Fernández-Llario, Pedroo
Segalés, Joaquim
Serrano, Emmanuel
author Gassó Garcia, Diana
author_facet Gassó Garcia, Diana
Vicente, Joaquín
Mentaberre García, Gregorio
Soriguer, Ramón
Jiménez Rodríguez, Rocío
Navarro González, Nora
Tvarijonaviciute, Asta
Lavín, Santiago
Fernández-Llario, Pedroo
Segalés, Joaquim
Serrano, Emmanuel
author_role author
author2 Vicente, Joaquín
Mentaberre García, Gregorio
Soriguer, Ramón
Jiménez Rodríguez, Rocío
Navarro González, Nora
Tvarijonaviciute, Asta
Lavín, Santiago
Fernández-Llario, Pedroo
Segalés, Joaquim
Serrano, Emmanuel
author2_role author
author
author
author
author
author
author
author
author
author
description Reactive oxygen and nitrogen species (ROS-RNS) are important defence substances involved in the immune response against pathogens. An excessive increase in ROS-RNS, however, can damage the organism causing oxidative stress (OS). The organism is able to neutralise OS by the production of antioxidant enzymes (AE); hence, tissue damage is the result of an imbalance between oxidant and antioxidant status. Though some work has been carried out in humans, there is a lack of information about the oxidant/antioxidant status in the presence of tuberculosis (TB) in wild reservoirs. In the Mediterranean Basin, wild boar (Sus scrofa) is the main reservoir of TB. Wild boar showing severe TB have an increased risk to Mycobacterium spp. shedding, leading to pathogen spreading and persistence. If OS is greater in these individuals, oxidant/antioxidant balance in TB-affected boars could be used as a biomarker of disease severity. The present work had a two-fold objective: i) to study the effects of bovine TB on different OS biomarkers (namely superoxide dismutase (SOD), catalasa (CAT), glutathione peroxidase (GPX), glutathione reductase (GR) and thiobarbituric acid reactive substances (TBARS)) in wild boar experimentally challenged with Mycobacterium bovis, and ii) to explore the role of body weight, sex, population and season in explaining the observed variability of OS indicators in two populations of free-ranging wild boar where TB is common. For the first objective, a partial least squares regression (PLSR) approach was used whereas, recursive partitioning with regression tree models (RTM) were applied for the second. A negative relationship between antioxidant enzymes and bovine TB (the more severe lesions, the lower the concentration of antioxidant biomarkers) was observed in experimentally infected animals. The final PLSR model permits retained the GPX, SOD and GR biomarkers and showed that 17.6% of the observed variability of antioxidant capacity was significantly correlated with the PLSR X's component represented by both disease status and the age of boars. In the samples from free-ranging wild boar, however, the environmental factors were more relevant to the observed variability of the OS biomarkers than the TB itself. For each OS biomarker, each RTM was defined as a maximum by one node due to the population effect. Along the same lines, the ad hoc tree regression on boars from the population with a higher prevalence of severe TB confirmed that disease status was not the main factor explaining the observed variability in OS biomarkers. It was concluded that oxidative damage caused by TB is significant, but can only be detected in the absence of environmental variation in wild boar.
publishDate 2016
dc.date.none.fl_str_mv 2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.1371/journal.pone.0163971
https://hdl.handle.net/10459.1/464920
url https://doi.org/10.1371/journal.pone.0163971
https://hdl.handle.net/10459.1/464920
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1371/journal.pone.0163971
Plos One, 2016, vol. 11, núm. 9, p. e0163971
dc.rights.none.fl_str_mv cc-by (c) Gassó et al., 2016
info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/4.0/
rights_invalid_str_mv cc-by (c) Gassó et al., 2016
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositori Obert UdL
instname:Universitat de Lleida (UdL)
instname_str Universitat de Lleida (UdL)
reponame_str Repositori Obert UdL
collection Repositori Obert UdL
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869421531491205120
score 15.811543

Similares en LA Referencia