Dissecting reactive astrocyte responses: lineage tracing and morphology-based clustering

Brain damage triggers diverse cellular and molecular events, with astrocytes playing a crucial role in activating local neuroprotective and reparative signaling within damaged neuronal circuits. Here, we investigated reactive astrocytes using a multidimensional approach to categorize their responses...

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Autores: Delgado-García, Lina M., Ojalvo-Sanz, Ana Cristina, Nakamura, Thabatta K.E., Martín-López, Eduardo, Porcionatto, Marimelia, López-Mascaraque, Laura
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2024
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositório:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/378075
Acesso em linha:http://hdl.handle.net/10261/378075
Access Level:Acceso aberto
Palavra-chave:Astrocyte lineages
Brain injury
Glia biology
Morphometrics
Reactive astrocyte response.
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spelling Dissecting reactive astrocyte responses: lineage tracing and morphology-based clusteringDelgado-García, Lina M.Ojalvo-Sanz, Ana CristinaNakamura, Thabatta K.E.Martín-López, EduardoPorcionatto, MarimeliaLópez-Mascaraque, LauraAstrocyte lineagesBrain injuryGlia biologyMorphometricsReactive astrocyte response.Brain damage triggers diverse cellular and molecular events, with astrocytes playing a crucial role in activating local neuroprotective and reparative signaling within damaged neuronal circuits. Here, we investigated reactive astrocytes using a multidimensional approach to categorize their responses into different subtypes based on morphology. This approach utilized the StarTrack lineage tracer, single-cell imaging reconstruction and multivariate data analysis. Our findings identified three profiles of reactive astrocyte responses, categorized by their effects on cell size- and shape- related morphological parameters: "moderate", "strong," and "very strong". We also examined the heterogeneity of astrocyte reactivity, focusing on spatial and clonal distribution. Our research revealed a notable enrichment of protoplasmic and fibrous astrocytes within the "strong" and "very strong" response subtypes. Overall, our study contributes to a better understanding of astrocyte heterogeneity in response to an injury. By characterizing the diverse reactive responses among astrocyte subpopulations, we provide insights that could guide future research aimed at identifying novel therapeutic targets to mitigate brain damage and promote neural repair.Open Access funding provided thanks to the CRUECSIC agreement with Springer Nature. This work was supported by MICINN Grant PID2019105218RB-I00 and MCIN/AEI/https://doi.org/10.13039/501100011033Grant/Award Number: PID2022136882NB-I00 in Spain, São Paulo Research Foundation, FAPESP Grants 16/19084–8, 19/09183–7, 18/12605–8 and 22/04258–1, Coordination for the Improvement of Higher Education Personnel, CAPES Financial Code 001, and National Council for Scientifc and Technological Development, CNPq INCT Model3D Grants 406258/2022–8 in Brazil. LMDG was supported by IBRO exchange fellowship program, 2020I; and ISN CAEN A1 program, 2022I.BioMed CentralConferencia de Rectores de las Universidades EspañolasSao Paulo Research FoundationConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2025202520242025info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/378075reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-105218RB-I00info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2022-136882NB-I00http://dx.doi.org/10.1186/s40659-024-00532-ySíinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3780752026-05-22T06:33:51Z
dc.title.none.fl_str_mv Dissecting reactive astrocyte responses: lineage tracing and morphology-based clustering
title Dissecting reactive astrocyte responses: lineage tracing and morphology-based clustering
spellingShingle Dissecting reactive astrocyte responses: lineage tracing and morphology-based clustering
Delgado-García, Lina M.
Astrocyte lineages
Brain injury
Glia biology
Morphometrics
Reactive astrocyte response.
title_short Dissecting reactive astrocyte responses: lineage tracing and morphology-based clustering
title_full Dissecting reactive astrocyte responses: lineage tracing and morphology-based clustering
title_fullStr Dissecting reactive astrocyte responses: lineage tracing and morphology-based clustering
title_full_unstemmed Dissecting reactive astrocyte responses: lineage tracing and morphology-based clustering
title_sort Dissecting reactive astrocyte responses: lineage tracing and morphology-based clustering
dc.creator.none.fl_str_mv Delgado-García, Lina M.
Ojalvo-Sanz, Ana Cristina
Nakamura, Thabatta K.E.
Martín-López, Eduardo
Porcionatto, Marimelia
López-Mascaraque, Laura
author Delgado-García, Lina M.
author_facet Delgado-García, Lina M.
Ojalvo-Sanz, Ana Cristina
Nakamura, Thabatta K.E.
Martín-López, Eduardo
Porcionatto, Marimelia
López-Mascaraque, Laura
author_role author
author2 Ojalvo-Sanz, Ana Cristina
Nakamura, Thabatta K.E.
Martín-López, Eduardo
Porcionatto, Marimelia
López-Mascaraque, Laura
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Conferencia de Rectores de las Universidades Españolas
Sao Paulo Research Foundation
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Astrocyte lineages
Brain injury
Glia biology
Morphometrics
Reactive astrocyte response.
topic Astrocyte lineages
Brain injury
Glia biology
Morphometrics
Reactive astrocyte response.
description Brain damage triggers diverse cellular and molecular events, with astrocytes playing a crucial role in activating local neuroprotective and reparative signaling within damaged neuronal circuits. Here, we investigated reactive astrocytes using a multidimensional approach to categorize their responses into different subtypes based on morphology. This approach utilized the StarTrack lineage tracer, single-cell imaging reconstruction and multivariate data analysis. Our findings identified three profiles of reactive astrocyte responses, categorized by their effects on cell size- and shape- related morphological parameters: "moderate", "strong," and "very strong". We also examined the heterogeneity of astrocyte reactivity, focusing on spatial and clonal distribution. Our research revealed a notable enrichment of protoplasmic and fibrous astrocytes within the "strong" and "very strong" response subtypes. Overall, our study contributes to a better understanding of astrocyte heterogeneity in response to an injury. By characterizing the diverse reactive responses among astrocyte subpopulations, we provide insights that could guide future research aimed at identifying novel therapeutic targets to mitigate brain damage and promote neural repair.
publishDate 2024
dc.date.none.fl_str_mv 2024
2025
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/378075
url http://hdl.handle.net/10261/378075
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-105218RB-I00
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2022-136882NB-I00
http://dx.doi.org/10.1186/s40659-024-00532-y
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
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repository.mail.fl_str_mv
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