The pLS20 family of plasmids and regulation of their establishment genes

Tesis doctoral inédita leída en la Universidad Autónoma de Madrid, Facultad de Ciencias, Departamento de Biología Molecular. Fecha de lectura: 25-02-2021

Detalles Bibliográficos
Autor: Val Calvo, Jorge
Tipo de recurso: tesis doctoral
Fecha de publicación:2021
País:España
Institución:Universidad Autónoma de Madrid
Repositorio:Biblos-e Archivo. Repositorio Institucional de la UAM
Idioma:inglés
OAI Identifier:oai:repositorio.uam.es:10486/694945
Acceso en línea:http://hdl.handle.net/10486/694945
Access Level:acceso abierto
Palabra clave:Plásmidos
Biología y Biomedicina / Biología
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dc.title.none.fl_str_mv The pLS20 family of plasmids and regulation of their establishment genes
La familia de plásmidos pLS20 y la regulación de sus genes de establecimiento
title The pLS20 family of plasmids and regulation of their establishment genes
spellingShingle The pLS20 family of plasmids and regulation of their establishment genes
Val Calvo, Jorge
Plásmidos
Biología y Biomedicina / Biología
title_short The pLS20 family of plasmids and regulation of their establishment genes
title_full The pLS20 family of plasmids and regulation of their establishment genes
title_fullStr The pLS20 family of plasmids and regulation of their establishment genes
title_full_unstemmed The pLS20 family of plasmids and regulation of their establishment genes
title_sort The pLS20 family of plasmids and regulation of their establishment genes
dc.creator.none.fl_str_mv Val Calvo, Jorge
author Val Calvo, Jorge
author_facet Val Calvo, Jorge
author_role author
dc.contributor.none.fl_str_mv Meijer, Wiflfried J.J.
Departamento de Biología Molecular
Facultad de Ciencias
UAM-CSIC. Centro de Biología Molecular Severo Ochoa (CBM)
dc.subject.none.fl_str_mv Plásmidos
Biología y Biomedicina / Biología
topic Plásmidos
Biología y Biomedicina / Biología
description Tesis doctoral inédita leída en la Universidad Autónoma de Madrid, Facultad de Ciencias, Departamento de Biología Molecular. Fecha de lectura: 25-02-2021
publishDate 2021
dc.date.none.fl_str_mv 2021
2021-02-25
dc.type.none.fl_str_mv doctoral thesis
http://purl.org/coar/resource_type/c_db06
NA
http://purl.org/coar/version/c_be7fb7dd8ff6fe43
dc.type.openaire.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
dc.identifier.none.fl_str_mv http://hdl.handle.net/10486/694945
url http://hdl.handle.net/10486/694945
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Biblos-e Archivo. Repositorio Institucional de la UAM
instname:Universidad Autónoma de Madrid
instname_str Universidad Autónoma de Madrid
reponame_str Biblos-e Archivo. Repositorio Institucional de la UAM
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spelling The pLS20 family of plasmids and regulation of their establishment genesLa familia de plásmidos pLS20 y la regulación de sus genes de establecimientoVal Calvo, JorgePlásmidosBiología y Biomedicina / BiologíaTesis doctoral inédita leída en la Universidad Autónoma de Madrid, Facultad de Ciencias, Departamento de Biología Molecular. Fecha de lectura: 25-02-2021Esta tesis tiene embargado el acceso al texto completo hasta el 25-08-2022The spread of antibiotic resistance genes (ARGs) is a major threat to global health. Horizontal gene transfer (HGT) events, and particularly the route of bacterial conjugation, are mainly responsible for the dissemination of ARGs. Conjugation is the process by which a conjugative element is transferred from a donor to a recipient cell through a connecting channel. Conjugative elements are often located on plasmids. Conjugative plasmids are widespread in Gram-positive (Gram+) and Gram-negative (Gram�) bacteria. Most conjugation studies are based on conjugative plasmids from Gram� bacteria though. In our laboratory, we study as a model the conjugative plasmid pLS20 from the Gram+ bacterium Bacillus subtilis. One main objective of this work was to determine the rareness of pLS20; is it a unique plasmid or does it belong to a family of related plasmids. Results obtained in these thesis studies show that pLS20 is the prototype of a new family of conjugative plasmids that is present in several Bacillus species isolated from diverse geographic locations and niches, suggesting that their distribution is worldwide. The pLS20 family of plasmids is characterised by an unusual (i) origin of replication region, that does not encode for a typical replication initiation protein, which is flanked on its left side by (ii) a partitioning system, and on its right side by (iii) a large conjugation operon. Interestingly, the conjugation module of pLS20 is also (partially) present on other Bacillus plasmids as well as on plasmids from other Gram+ bacteria including pathogens such as Listeria, Enterococcus and Clostridium species, indicating that this conjugation module is widely distributed among conjugative plasmids hosted by bacteria of the phylum Firmicutes. Bacteria contain systems to defend themselves against incoming foreign DNA. Establishment of a plasmid in the recipient host after conjugative transfer requires a transient inactivation of these host-defence mechanisms. Many conjugative plasmids contain so-called establishment genes that fulfil this role. In this doctoral thesis, I identified the putative establishment genes present on members of the pLS20 family of plasmids. The establishment genes on these plasmids are organised in a regulon that is composed of several operons. Remarkably, there is a wide variation in the number and kind of establishment genes on the different plasmids. Two fundamentally different regulatory mechanisms control the expression of these regulons. Plasmids pLS20 and p576 are the two prototype plasmids reflecting these alternative mechanisms. In the case of p576, the plasmid encodes a repressor protein (called Reg) that effectively suppresses the establishment promoters. During the conjugation, the DNA but not the repressor is transferred. Consequently, the establishment promoters are transiently active in the recipient cell until sufficient repressor has been synthesised to repress them again. In the case of pLS20, the establishment genes are preceded by a conserved large upstream element (CLUE) that most likely is responsible for transient expression of the establishment genes by a yet unknown mechanismMeijer, Wiflfried J.J.Departamento de Biología MolecularFacultad de CienciasUAM-CSIC. Centro de Biología Molecular Severo Ochoa (CBM)20212021-02-25doctoral thesishttp://purl.org/coar/resource_type/c_db06NAhttp://purl.org/coar/version/c_be7fb7dd8ff6fe43info:eu-repo/semantics/doctoralThesisapplication/pdfhttp://hdl.handle.net/10486/694945reponame:Biblos-e Archivo. Repositorio Institucional de la UAMinstname:Universidad Autónoma de MadridInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:repositorio.uam.es:10486/6949452026-06-23T12:46:27Z
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