Experimental infection of sheep at mid-pregnancy with archetypal type II and type III Toxoplasma gondii isolates exhibited different phenotypic traits

12 páginas, 4 figuras, 4 tablas.

Bibliographic Details
Authors: Vallejo, R., Benavides, Julio, Arteche-Villasol, Noive, Sánchez-Sánchez, R., Calero-Bernal, R., Ferreras, Mª del Carmen, Criado, M., Pérez Pérez, Valentín, Ortega-Mora, L.M., Gutiérrez-Expósito, Daniel
Format: article
Status:Published version
Publication Date:2023
Country:España
Institution:Consejo Superior de Investigaciones Científicas (CSIC)
Repository:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/311009
Online Access:http://hdl.handle.net/10261/311009
Access Level:Open access
Keyword:Toxoplasma gondii
Oocysts
Genotype
Pregnant sheep
Phenotypic traits
http://metadata.un.org/sdg/12
Ensure sustainable consumption and production patterns
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dc.title.none.fl_str_mv Experimental infection of sheep at mid-pregnancy with archetypal type II and type III Toxoplasma gondii isolates exhibited different phenotypic traits
title Experimental infection of sheep at mid-pregnancy with archetypal type II and type III Toxoplasma gondii isolates exhibited different phenotypic traits
spellingShingle Experimental infection of sheep at mid-pregnancy with archetypal type II and type III Toxoplasma gondii isolates exhibited different phenotypic traits
Vallejo, R.
Toxoplasma gondii
Oocysts
Genotype
Pregnant sheep
Phenotypic traits
http://metadata.un.org/sdg/12
Ensure sustainable consumption and production patterns
title_short Experimental infection of sheep at mid-pregnancy with archetypal type II and type III Toxoplasma gondii isolates exhibited different phenotypic traits
title_full Experimental infection of sheep at mid-pregnancy with archetypal type II and type III Toxoplasma gondii isolates exhibited different phenotypic traits
title_fullStr Experimental infection of sheep at mid-pregnancy with archetypal type II and type III Toxoplasma gondii isolates exhibited different phenotypic traits
title_full_unstemmed Experimental infection of sheep at mid-pregnancy with archetypal type II and type III Toxoplasma gondii isolates exhibited different phenotypic traits
title_sort Experimental infection of sheep at mid-pregnancy with archetypal type II and type III Toxoplasma gondii isolates exhibited different phenotypic traits
dc.creator.none.fl_str_mv Vallejo, R.
Benavides, Julio
Arteche-Villasol, Noive
Sánchez-Sánchez, R.
Calero-Bernal, R.
Ferreras, Mª del Carmen
Criado, M.
Pérez Pérez, Valentín
Ortega-Mora, L.M.
Gutiérrez-Expósito, Daniel
author Vallejo, R.
author_facet Vallejo, R.
Benavides, Julio
Arteche-Villasol, Noive
Sánchez-Sánchez, R.
Calero-Bernal, R.
Ferreras, Mª del Carmen
Criado, M.
Pérez Pérez, Valentín
Ortega-Mora, L.M.
Gutiérrez-Expósito, Daniel
author_role author
author2 Benavides, Julio
Arteche-Villasol, Noive
Sánchez-Sánchez, R.
Calero-Bernal, R.
Ferreras, Mª del Carmen
Criado, M.
Pérez Pérez, Valentín
Ortega-Mora, L.M.
Gutiérrez-Expósito, Daniel
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Junta de Castilla y León
European Commission
Ministerio de Economía, Industria y Competitividad (España)
Agencia Estatal de Investigación (España)
Ministerio de Ciencia e Innovación (España)
Benavides, Julio [0000-0001-9706-100X]
Arteche Villasol, Noive [0000-0001-5793-9578]
Ferreras Estrada, Mª del Carmen [0000-0003-1996-2229]
Pérez Pérez, Valentín [0000-0003-0075-1587]
Gutiérrez Expósito, Daniel [0000-0002-7683-623X]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Toxoplasma gondii
Oocysts
Genotype
Pregnant sheep
Phenotypic traits
http://metadata.un.org/sdg/12
Ensure sustainable consumption and production patterns
topic Toxoplasma gondii
Oocysts
Genotype
Pregnant sheep
Phenotypic traits
http://metadata.un.org/sdg/12
Ensure sustainable consumption and production patterns
description 12 páginas, 4 figuras, 4 tablas.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/311009
url http://hdl.handle.net/10261/311009
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
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info:eu-repo/grantAgreement/AEI//AGL2016-75935-C2-2-R
info:eu-repo/grantAgreement/AEI//BES-2016-076513
info:eu-repo/grantAgreement/AEI//PRE2019–087309
http://dx.doi.org/10.1016/j.vetpar.2023.109889
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dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
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spelling Experimental infection of sheep at mid-pregnancy with archetypal type II and type III Toxoplasma gondii isolates exhibited different phenotypic traitsVallejo, R.Benavides, JulioArteche-Villasol, NoiveSánchez-Sánchez, R.Calero-Bernal, R.Ferreras, Mª del CarmenCriado, M.Pérez Pérez, ValentínOrtega-Mora, L.M.Gutiérrez-Expósito, DanielToxoplasma gondiiOocystsGenotypePregnant sheepPhenotypic traitshttp://metadata.un.org/sdg/12Ensure sustainable consumption and production patterns12 páginas, 4 figuras, 4 tablas.Toxoplasma gondii is a major cause of reproductive failure in small ruminants. Genotypic diversity of T. gondii strains has been associated with variations in phenotypic traits in in vitro and murine models. However, whether such diversity could influence the outcome of infection in small ruminants remains mostly unexplored. Here, we investigate the outcome of oral challenge in sheep at mid-pregnancy with 10 sporulated oocysts from three different T. gondii isolates belonging to archetypal II and III and selected according to their genetic and phenotypic variations shown in previous studies. Seventy-three pregnant sheep were divided in four groups: G1 infected with TgShSp1 isolate (type II, ToxoDB#3), G2 with TgShSp16 isolate (type II, ToxoDB#3), G3 with TgShSp24 isolate (type III, ToxoDB#2) and G4 of uninfected control sheep. Two different approaches were carried out within this study: (i) the outcome for the pregnancy after infection (n = 33) and (ii) the lesions and parasite tropism and burden at 14 and 28 days post infection (dpi) (n = 40). The onset of hyperthermia and seroconversion occurred one and two days later, respectively in G1 when compared to G2 and G3. However, sheep that suffered from reproductive failure, either by abortion, foetal dead at the time of euthanasia or stillbirth were similar among infected groups (50%, 40% and 47%, respectively). Histological lesions in placentomes and foetal tissues from euthanized animals from the second approach were only detected at 28 dpi and mainly in G1. At 14 dpi, T. gondii-DNA was only detected in G1 in the 11% of the placentomes. However, at 28 dpi the frequency of detection in placentomes was higher in G1 (96%) than in G2 and G3 (7% and 47%, respectively) besides in foetuses was lower in G2 (20%) than in G1 and G3 (100% and 87%, respectively). Regarding late abortions, stillbirths, and lambs of G1, G2 and G3, the frequency of microscopic lesions was similar between groups (79%, 78% and 67%, respectively) whereas T. gondii-DNA was evidenced in 100%, 55% and 100%, respectively. These recently obtained T. gondii isolates led to similar reproductive losses but intra- and intergenotype variations in the rise of hyperthermia, dynamics of antibodies, frequency of lesions and parasite detection and distribution. Thus, the different phenotypic traits of the isolates could influence the outcome of the infection and mechanisms responsible for it, and further investigations are warranted.This work was supported by research projects from the “Junta de Castilla y Leon´ ” (LE080U16) and FEDER/Spanish Ministry of Economy, Industry and Competitiveness-Spanish State Research Agency, AEI (AGL2016-75935-C2-1-R and AGL2016-75935-C2-2-R).Raquel Vallejo, Noive Arteche-Villasol and Miguel Criado are the recipients of a predoctoral contract from the Ministry of Science and Innovation (grant no. FPU-1705346, BES-2016076513 and PRE2019–087309, respectively).Peer reviewedElsevierJunta de Castilla y LeónEuropean CommissionMinisterio de Economía, Industria y Competitividad (España)Agencia Estatal de Investigación (España)Ministerio de Ciencia e Innovación (España)Benavides, Julio [0000-0001-9706-100X]Arteche Villasol, Noive [0000-0001-5793-9578]Ferreras Estrada, Mª del Carmen [0000-0003-1996-2229]Pérez Pérez, Valentín [0000-0003-0075-1587]Gutiérrez Expósito, Daniel [0000-0002-7683-623X]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/311009reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI//AGL2016-75935-C2-1-Rinfo:eu-repo/grantAgreement/AEI//AGL2016-75935-C2-2-Rinfo:eu-repo/grantAgreement/AEI//BES-2016-076513info:eu-repo/grantAgreement/AEI//PRE2019–087309http://dx.doi.org/10.1016/j.vetpar.2023.109889Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3110092026-05-22T06:33:51Z
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