Revision and reannotation of the Halomonas elongata DSM 2581T genoma
The genome of the Halomonas elongata type strain DSM 2581, an industrial producer, was reevaluated using the Illumina HiSeq2500 technology. To resolve duplication-associated ambiguities, PCR products were generated and sequenced. Outside of duplications, 72 sequence corrections were required, of whi...
| Autores: | , , , , , , , |
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| Formato: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2017 |
| País: | España |
| Recursos: | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| Repositorio: | Recercat. Dipósit de la Recerca de Catalunya |
| OAI Identifier: | oai:recercat.cat:10459.1/467725 |
| Acesso em linha: | https://doi.org/10.1002/mbo3.465 https://hdl.handle.net/10459.1/467725 |
| Access Level: | acceso abierto |
| Palavra-chave: | Frameshift Genome annotation Genome sequencing Halomonas elongata Halophilic bacteria Sequence revision |
| Resumo: | The genome of the Halomonas elongata type strain DSM 2581, an industrial producer, was reevaluated using the Illumina HiSeq2500 technology. To resolve duplication-associated ambiguities, PCR products were generated and sequenced. Outside of duplications, 72 sequence corrections were required, of which 24 were point mutations and 48 were indels of one or few bases. Most of these were associated with polynucleotidestretches (poly-T stretch overestimated in 19 cases, poly-Cunderestimated in 15 cases). These problems may be attributed to using 454 technology for original genoma sequencing. On average, the original genome sequence had only one error in 56 kb. There 10:01 24/3/2025were 23 frameshift error corrections in the 29 protein-coding genes affected by sequence revision. The genome has been subjected to major reannotation in order to substantially increase the annotation quality. |
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