Volatile-mediated interactions between Trichoderma harzianum and Acanthoscelides obtectus: A novel in vitro methodology to evaluate the impact of microbial volatile compounds on dry grain storage pests
[EN] Biological interactions mediated by Biogenic Volatile Organic Compounds (BVOCs) is a well-established field that has been researched for decades. Although extensive focus is currently given to the control of insect pests using natural molecules, the study of volatile interactions between microo...
| Autores: | , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2022 |
| País: | España |
| Institución: | Universidad Rey Juan Carlos |
| Repositorio: | BULERIA. Repositorio Institucional de la Universidad de León |
| OAI Identifier: | oai:buleria.unileon.es:10612/14451 |
| Acceso en línea: | https://www.sciencedirect.com/science/article/pii/S1049964422000330?via%3Dihub#! http://hdl.handle.net/10612/14451 |
| Access Level: | acceso abierto |
| Palabra clave: | Ingeniería agrícola Trichoderma Acanthoscelides VOC Trichodiene Bean VOC Chamber 31 Ciencias Agrarias agra |
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Volatile-mediated interactions between Trichoderma harzianum and Acanthoscelides obtectus: A novel in vitro methodology to evaluate the impact of microbial volatile compounds on dry grain storage pestsÁlvarez García, SamuelRodríguez González, ÁlvaroZanfaño, LauraGutiérrez , Santiago 1965-Casquero Luelmo, Pedro AntonioIngeniería agrícolaTrichodermaAcanthoscelidesVOCTrichodieneBeanVOC Chamber31 Ciencias Agrariasagra[EN] Biological interactions mediated by Biogenic Volatile Organic Compounds (BVOCs) is a well-established field that has been researched for decades. Although extensive focus is currently given to the control of insect pests using natural molecules, the study of volatile interactions between microorganisms and insects has been largely neglected and has only begun to attract more attention in recent years. In this work, we developed a novel protocol to assess the effects of microbial BVOCs directly produced by growing microbial strains on dry grain insect pests and the seed damage they cause, using VOC Chambers to evaluate both sealed and unsealed conditions. Four Trichoderma harzianum strains were tested against Acanthoscelides obtectus, a wild type and three of its transformants. These had been previously obtained by introducing the tri5 gene and thus overproducing the volatile trichodiene, or by silencing the erg1 gene, which encodes for a squalene epoxidase, therefore reducing ergosterol levels and increasing squalene ones in the fungus. Results demonstrated that ventilation plays a key role in these interactions. All fungal strains significantly increased adult mortality in sealed conditions, while this effect was barely noticeable in unsealed ones. Nevertheless, subsequent insect emergence from bean seeds and bean damage were still significantly reduced in both conditions. The erg1 silenced strains caused significantly higher levels of adult mortality than the rest in sealed conditions and lower insect emergence in both sealed and unsealed ones. Bean damage produced by insects was lower also when exposed to BVOCs from these strains in sealed conditions. Conversely, trichodiene overproduction did not show enhanced toxicity or significant reduction of insect emergence and bean damage in the tested conditions. Therefore, T. harzianum BVOCs, especially those from erg1 silenced strains, should be further researched for their potential use in the biological control of A. obtectus infestation in dry grain storing facilities. VOC Chambers have shown themselves to be a reliable method in the screening of in vitro volatile mediated interactions between growing microbial strains and insect pests.SIElsevierProduccion VegetalEscuela de Ingeniería Agraria y Forestal2022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://www.sciencedirect.com/science/article/pii/S1049964422000330?via%3Dihub#!http://hdl.handle.net/10612/14451reponame:BULERIA. Repositorio Institucional de la Universidad de Leóninstname:Universidad Rey Juan CarlosInglésJunta de Castilla y León LE251P18http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:buleria.unileon.es:10612/144512026-06-24T12:43:27Z |
| dc.title.none.fl_str_mv |
Volatile-mediated interactions between Trichoderma harzianum and Acanthoscelides obtectus: A novel in vitro methodology to evaluate the impact of microbial volatile compounds on dry grain storage pests |
| title |
Volatile-mediated interactions between Trichoderma harzianum and Acanthoscelides obtectus: A novel in vitro methodology to evaluate the impact of microbial volatile compounds on dry grain storage pests |
| spellingShingle |
Volatile-mediated interactions between Trichoderma harzianum and Acanthoscelides obtectus: A novel in vitro methodology to evaluate the impact of microbial volatile compounds on dry grain storage pests Álvarez García, Samuel Ingeniería agrícola Trichoderma Acanthoscelides VOC Trichodiene Bean VOC Chamber 31 Ciencias Agrarias agra |
| title_short |
Volatile-mediated interactions between Trichoderma harzianum and Acanthoscelides obtectus: A novel in vitro methodology to evaluate the impact of microbial volatile compounds on dry grain storage pests |
| title_full |
Volatile-mediated interactions between Trichoderma harzianum and Acanthoscelides obtectus: A novel in vitro methodology to evaluate the impact of microbial volatile compounds on dry grain storage pests |
| title_fullStr |
Volatile-mediated interactions between Trichoderma harzianum and Acanthoscelides obtectus: A novel in vitro methodology to evaluate the impact of microbial volatile compounds on dry grain storage pests |
| title_full_unstemmed |
Volatile-mediated interactions between Trichoderma harzianum and Acanthoscelides obtectus: A novel in vitro methodology to evaluate the impact of microbial volatile compounds on dry grain storage pests |
| title_sort |
Volatile-mediated interactions between Trichoderma harzianum and Acanthoscelides obtectus: A novel in vitro methodology to evaluate the impact of microbial volatile compounds on dry grain storage pests |
| dc.creator.none.fl_str_mv |
Álvarez García, Samuel Rodríguez González, Álvaro Zanfaño, Laura Gutiérrez , Santiago 1965- Casquero Luelmo, Pedro Antonio |
| author |
Álvarez García, Samuel |
| author_facet |
Álvarez García, Samuel Rodríguez González, Álvaro Zanfaño, Laura Gutiérrez , Santiago 1965- Casquero Luelmo, Pedro Antonio |
| author_role |
author |
| author2 |
Rodríguez González, Álvaro Zanfaño, Laura Gutiérrez , Santiago 1965- Casquero Luelmo, Pedro Antonio |
| author2_role |
author author author author |
| dc.contributor.none.fl_str_mv |
Produccion Vegetal Escuela de Ingeniería Agraria y Forestal |
| dc.subject.none.fl_str_mv |
Ingeniería agrícola Trichoderma Acanthoscelides VOC Trichodiene Bean VOC Chamber 31 Ciencias Agrarias agra |
| topic |
Ingeniería agrícola Trichoderma Acanthoscelides VOC Trichodiene Bean VOC Chamber 31 Ciencias Agrarias agra |
| description |
[EN] Biological interactions mediated by Biogenic Volatile Organic Compounds (BVOCs) is a well-established field that has been researched for decades. Although extensive focus is currently given to the control of insect pests using natural molecules, the study of volatile interactions between microorganisms and insects has been largely neglected and has only begun to attract more attention in recent years. In this work, we developed a novel protocol to assess the effects of microbial BVOCs directly produced by growing microbial strains on dry grain insect pests and the seed damage they cause, using VOC Chambers to evaluate both sealed and unsealed conditions. Four Trichoderma harzianum strains were tested against Acanthoscelides obtectus, a wild type and three of its transformants. These had been previously obtained by introducing the tri5 gene and thus overproducing the volatile trichodiene, or by silencing the erg1 gene, which encodes for a squalene epoxidase, therefore reducing ergosterol levels and increasing squalene ones in the fungus. Results demonstrated that ventilation plays a key role in these interactions. All fungal strains significantly increased adult mortality in sealed conditions, while this effect was barely noticeable in unsealed ones. Nevertheless, subsequent insect emergence from bean seeds and bean damage were still significantly reduced in both conditions. The erg1 silenced strains caused significantly higher levels of adult mortality than the rest in sealed conditions and lower insect emergence in both sealed and unsealed ones. Bean damage produced by insects was lower also when exposed to BVOCs from these strains in sealed conditions. Conversely, trichodiene overproduction did not show enhanced toxicity or significant reduction of insect emergence and bean damage in the tested conditions. Therefore, T. harzianum BVOCs, especially those from erg1 silenced strains, should be further researched for their potential use in the biological control of A. obtectus infestation in dry grain storing facilities. VOC Chambers have shown themselves to be a reliable method in the screening of in vitro volatile mediated interactions between growing microbial strains and insect pests. |
| publishDate |
2022 |
| dc.date.none.fl_str_mv |
2022 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
https://www.sciencedirect.com/science/article/pii/S1049964422000330?via%3Dihub#! http://hdl.handle.net/10612/14451 |
| url |
https://www.sciencedirect.com/science/article/pii/S1049964422000330?via%3Dihub#! http://hdl.handle.net/10612/14451 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
Junta de Castilla y León LE251P18 |
| dc.rights.none.fl_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| eu_rights_str_mv |
openAccess |
| dc.publisher.none.fl_str_mv |
Elsevier |
| publisher.none.fl_str_mv |
Elsevier |
| dc.source.none.fl_str_mv |
reponame:BULERIA. Repositorio Institucional de la Universidad de León instname:Universidad Rey Juan Carlos |
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Universidad Rey Juan Carlos |
| reponame_str |
BULERIA. Repositorio Institucional de la Universidad de León |
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BULERIA. Repositorio Institucional de la Universidad de León |
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15,300719 |