Neurohormonal activation induces intracellular iron deficiency and mitochondrial dysfunction in cardiac cells

Cèl·lula cardíaca; Deficiència de ferro; Activació neurohormonal

Detalles Bibliográficos
Autores: Tajes, M., Díez‑López, C., Enjuanes, Cristina, Moliner, Pedro, Ferreiro, José Luis, Garay, A., Yañez Bisbe, Laia, Benito Villabriga, Begoña
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Departament de Salut de la Generalitat de Catalunya (DS)
Repositorio:Scientia. Dipòsit d'Informació Digital del Departament de Salut
OAI Identifier:oai:scientiasalut.gencat.cat:11351/6770
Acceso en línea:https://hdl.handle.net/11351/6770
Access Level:acceso abierto
Palabra clave:Anèmia ferropènica
Insuficiència cardíaca
DISEASES::Hemic and Lymphatic Diseases::Hematologic Diseases::Anemia::Anemia, Hypochromic::Anemia, Iron-Deficiency
DISEASES::Cardiovascular Diseases::Heart Diseases::Heart Failure
CHEMICALS AND DRUGS::Amino Acids, Peptides, and Proteins::Proteins::Iron-Regulatory Proteins
ENFERMEDADES::enfermedades hematológicas y linfáticas::enfermedades hematológicas::anemia::anemia hipocrómica::anemia ferropénica
ENFERMEDADES::enfermedades cardiovasculares::enfermedades cardíacas::insuficiencia cardíaca
COMPUESTOS QUÍMICOS Y DROGAS::aminoácidos, péptidos y proteínas::proteínas::proteínas reguladoras del hierro
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network_acronym_str ES
network_name_str España
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dc.title.none.fl_str_mv Neurohormonal activation induces intracellular iron deficiency and mitochondrial dysfunction in cardiac cells
title Neurohormonal activation induces intracellular iron deficiency and mitochondrial dysfunction in cardiac cells
spellingShingle Neurohormonal activation induces intracellular iron deficiency and mitochondrial dysfunction in cardiac cells
Tajes, M.
Anèmia ferropènica
Insuficiència cardíaca
DISEASES::Hemic and Lymphatic Diseases::Hematologic Diseases::Anemia::Anemia, Hypochromic::Anemia, Iron-Deficiency
DISEASES::Cardiovascular Diseases::Heart Diseases::Heart Failure
CHEMICALS AND DRUGS::Amino Acids, Peptides, and Proteins::Proteins::Iron-Regulatory Proteins
ENFERMEDADES::enfermedades hematológicas y linfáticas::enfermedades hematológicas::anemia::anemia hipocrómica::anemia ferropénica
ENFERMEDADES::enfermedades cardiovasculares::enfermedades cardíacas::insuficiencia cardíaca
COMPUESTOS QUÍMICOS Y DROGAS::aminoácidos, péptidos y proteínas::proteínas::proteínas reguladoras del hierro
title_short Neurohormonal activation induces intracellular iron deficiency and mitochondrial dysfunction in cardiac cells
title_full Neurohormonal activation induces intracellular iron deficiency and mitochondrial dysfunction in cardiac cells
title_fullStr Neurohormonal activation induces intracellular iron deficiency and mitochondrial dysfunction in cardiac cells
title_full_unstemmed Neurohormonal activation induces intracellular iron deficiency and mitochondrial dysfunction in cardiac cells
title_sort Neurohormonal activation induces intracellular iron deficiency and mitochondrial dysfunction in cardiac cells
dc.creator.none.fl_str_mv Tajes, M.
Díez‑López, C.
Enjuanes, Cristina
Moliner, Pedro
Ferreiro, José Luis
Garay, A.
Yañez Bisbe, Laia
Benito Villabriga, Begoña
author Tajes, M.
author_facet Tajes, M.
Díez‑López, C.
Enjuanes, Cristina
Moliner, Pedro
Ferreiro, José Luis
Garay, A.
Yañez Bisbe, Laia
Benito Villabriga, Begoña
author_role author
author2 Díez‑López, C.
Enjuanes, Cristina
Moliner, Pedro
Ferreiro, José Luis
Garay, A.
Yañez Bisbe, Laia
Benito Villabriga, Begoña
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Institut Català de la Salut
[Tajes M, Ferreiro JL] Bio Heart Cardiovascular Diseases Research Group, Bellvitge Biomedical Research Institute (IDIBELL), L’Hospitalet de Llobregat, Barcelona, Spain. Cardiology Department, Bellvitge University Hospital, L’Hospitalet de Llobregat, Barcelona, Spain. [Díez-López C] Bio Heart Cardiovascular Diseases Research Group, Bellvitge Biomedical Research Institute (IDIBELL), L’Hospitalet de Llobregat, Barcelona, Spain. Cardiology Department, Bellvitge University Hospital, L’Hospitalet de Llobregat, Barcelona, Spain. Advanced Heart Failure and Heart Transplant Unit, Cardiology Department, Bellvitge University Hospital, L’Hospitalet de Llobregat, Barcelona, Spain. Department of Clinical Sciences, School of Medicine, University of Barcelona, Barcelona, Spain. [Enjuanes C, Moliner P, Garay A] Bio Heart Cardiovascular Diseases Research Group, Bellvitge Biomedical Research Institute (IDIBELL), L’Hospitalet de Llobregat, Barcelona, Spain. Cardiology Department, Bellvitge University Hospital, L’Hospitalet de Llobregat, Barcelona, Spain. Community Heart Failure Program, Cardiology Department, Bellvitge University Hospital, L’Hospitalet de Llobregat, Barcelona, Spain. [Yañez-Bisbe L] Programa de Biologia Vascular i Metabolisme, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. [Benito B] Programa de Biologia Vascular i Metabolisme, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Servei de Cardiologia, Vall d’Hebron Hospital Universitari, Barcelona, Spain. Departament de Medicina, Universitat Autònoma de Barcelona, Bellaterra, Spain
Vall d'Hebron Barcelona Hospital Campus
dc.subject.none.fl_str_mv Anèmia ferropènica
Insuficiència cardíaca
DISEASES::Hemic and Lymphatic Diseases::Hematologic Diseases::Anemia::Anemia, Hypochromic::Anemia, Iron-Deficiency
DISEASES::Cardiovascular Diseases::Heart Diseases::Heart Failure
CHEMICALS AND DRUGS::Amino Acids, Peptides, and Proteins::Proteins::Iron-Regulatory Proteins
ENFERMEDADES::enfermedades hematológicas y linfáticas::enfermedades hematológicas::anemia::anemia hipocrómica::anemia ferropénica
ENFERMEDADES::enfermedades cardiovasculares::enfermedades cardíacas::insuficiencia cardíaca
COMPUESTOS QUÍMICOS Y DROGAS::aminoácidos, péptidos y proteínas::proteínas::proteínas reguladoras del hierro
topic Anèmia ferropènica
Insuficiència cardíaca
DISEASES::Hemic and Lymphatic Diseases::Hematologic Diseases::Anemia::Anemia, Hypochromic::Anemia, Iron-Deficiency
DISEASES::Cardiovascular Diseases::Heart Diseases::Heart Failure
CHEMICALS AND DRUGS::Amino Acids, Peptides, and Proteins::Proteins::Iron-Regulatory Proteins
ENFERMEDADES::enfermedades hematológicas y linfáticas::enfermedades hematológicas::anemia::anemia hipocrómica::anemia ferropénica
ENFERMEDADES::enfermedades cardiovasculares::enfermedades cardíacas::insuficiencia cardíaca
COMPUESTOS QUÍMICOS Y DROGAS::aminoácidos, péptidos y proteínas::proteínas::proteínas reguladoras del hierro
description Cèl·lula cardíaca; Deficiència de ferro; Activació neurohormonal
publishDate 2021
dc.date.none.fl_str_mv 2021
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11351/6770
url https://hdl.handle.net/11351/6770
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Cell and Bioscience;11
https://doi.org/10.1186/s13578-021-00605-5
dc.rights.none.fl_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv BMC
publisher.none.fl_str_mv BMC
dc.source.none.fl_str_mv Scientia
reponame:Scientia. Dipòsit d'Informació Digital del Departament de Salut
instname:Departament de Salut de la Generalitat de Catalunya (DS)
instname_str Departament de Salut de la Generalitat de Catalunya (DS)
reponame_str Scientia. Dipòsit d'Informació Digital del Departament de Salut
collection Scientia. Dipòsit d'Informació Digital del Departament de Salut
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869421407131140096
spelling Neurohormonal activation induces intracellular iron deficiency and mitochondrial dysfunction in cardiac cellsTajes, M.Díez‑López, C.Enjuanes, CristinaMoliner, PedroFerreiro, José LuisGaray, A.Yañez Bisbe, LaiaBenito Villabriga, BegoñaAnèmia ferropènicaInsuficiència cardíacaDISEASES::Hemic and Lymphatic Diseases::Hematologic Diseases::Anemia::Anemia, Hypochromic::Anemia, Iron-DeficiencyDISEASES::Cardiovascular Diseases::Heart Diseases::Heart FailureCHEMICALS AND DRUGS::Amino Acids, Peptides, and Proteins::Proteins::Iron-Regulatory ProteinsENFERMEDADES::enfermedades hematológicas y linfáticas::enfermedades hematológicas::anemia::anemia hipocrómica::anemia ferropénicaENFERMEDADES::enfermedades cardiovasculares::enfermedades cardíacas::insuficiencia cardíacaCOMPUESTOS QUÍMICOS Y DROGAS::aminoácidos, péptidos y proteínas::proteínas::proteínas reguladoras del hierroCèl·lula cardíaca; Deficiència de ferro; Activació neurohormonalCardiac cell; Iron deficiency; Neurohormonal activationCélula cardíaca; Deficiencia de hierro; Activación neurohormonalBackground Iron deficiency (ID) is common in patients with heart failure (HF) and is associated with poor outcomes, yet its role in the pathophysiology of HF is not well-defined. We sought to determine the consequences of HF neurohormonal activation in iron homeostasis and mitochondrial function in cardiac cells. Methods HF was induced in C57BL/6 mice by using isoproterenol osmotic pumps and embryonic rat heart-derived H9c2 cells were subsequently challenged with Angiotensin II and/or Norepinephrine. The expression of several genes and proteins related to intracellular iron metabolism were assessed by Real time-PCR and immunoblotting, respectively. The intracellular iron levels were also determined. Mitochondrial function was analyzed by studying the mitochondrial membrane potential, the accumulation of radical oxygen species (ROS) and the adenosine triphosphate (ATP) production. Results Hearts from isoproterenol-stimulated mice showed a decreased in both mRNA and protein levels of iron regulatory proteins, transferrin receptor 1, ferroportin 1 and hepcidin compared to control mice. Furthermore, mitoferrin 2 and mitochondrial ferritin were also downregulated in the hearts from HF mice. Similar data regarding these key iron regulatory molecules were found in the H9c2 cells challenged with neurohormonal stimuli. Accordingly, a depletion of intracellular iron levels was found in the stimulated cells compared to non-stimulated cells, as well as in the hearts from the isoproterenol-induced HF mice. Finally, neurohormonal activation impaired mitochondrial function as indicated by the accumulation of ROS, the impaired mitochondrial membrane potential and the decrease in the ATP levels in the cardiac cells. Conclusions HF characteristic neurohormonal activation induced changes in the regulation of key molecules involved in iron homeostasis, reduced intracellular iron levels and impaired mitochondrial function. The current results suggest that iron could be involved in the pathophysiology of HF.This work was funded by the following Grants: unrestricted grant from Vifor Pharma and Basic Research Competitive Grant in Cardiology from the Spanish Society of Cardiology 2015.BMCInstitut Català de la Salut[Tajes M, Ferreiro JL] Bio Heart Cardiovascular Diseases Research Group, Bellvitge Biomedical Research Institute (IDIBELL), L’Hospitalet de Llobregat, Barcelona, Spain. Cardiology Department, Bellvitge University Hospital, L’Hospitalet de Llobregat, Barcelona, Spain. [Díez-López C] Bio Heart Cardiovascular Diseases Research Group, Bellvitge Biomedical Research Institute (IDIBELL), L’Hospitalet de Llobregat, Barcelona, Spain. Cardiology Department, Bellvitge University Hospital, L’Hospitalet de Llobregat, Barcelona, Spain. Advanced Heart Failure and Heart Transplant Unit, Cardiology Department, Bellvitge University Hospital, L’Hospitalet de Llobregat, Barcelona, Spain. Department of Clinical Sciences, School of Medicine, University of Barcelona, Barcelona, Spain. [Enjuanes C, Moliner P, Garay A] Bio Heart Cardiovascular Diseases Research Group, Bellvitge Biomedical Research Institute (IDIBELL), L’Hospitalet de Llobregat, Barcelona, Spain. Cardiology Department, Bellvitge University Hospital, L’Hospitalet de Llobregat, Barcelona, Spain. Community Heart Failure Program, Cardiology Department, Bellvitge University Hospital, L’Hospitalet de Llobregat, Barcelona, Spain. [Yañez-Bisbe L] Programa de Biologia Vascular i Metabolisme, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. [Benito B] Programa de Biologia Vascular i Metabolisme, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Servei de Cardiologia, Vall d’Hebron Hospital Universitari, Barcelona, Spain. Departament de Medicina, Universitat Autònoma de Barcelona, Bellaterra, SpainVall d'Hebron Barcelona Hospital Campus202220222021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/11351/6770Scientiareponame:Scientia. Dipòsit d'Informació Digital del Departament de Salutinstname:Departament de Salut de la Generalitat de Catalunya (DS)InglésCell and Bioscience;11https://doi.org/10.1186/s13578-021-00605-5Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:scientiasalut.gencat.cat:11351/67702026-06-12T09:38:37Z
score 15.812429

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