Nasopharyngeal Panbio COVID-19 Antigen Performed at Point-of-Care Has a High Sensitivity in Symptomatic and Asymptomatic Patients With Higher Risk for Transmission and Older Age

Background. Performance of point-of-care tests in different clinical scenarios and on different samples remains undetermined. We comprehensively evaluated the performance of the nasopharyngeal Panbio COVID-19 Ag Rapid Test Device. Methods. This is a prospective study that includes consecutive patien...

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Autores: Masiá, Mar, Fernández-González, Marta, Sánchez-Molla, Manuel, Carvajal, Mar, García, José Alberto, Gonzalo Jiménez, Nieves, Ortiz de la Tabla, Victoria, Agulló, Vanesa, Candela, Inmaculada, Guijarro, Jorge, Gutiérrez, José Antonio, de Gregorio, Carlos, Gutiérrez, Félix
Tipo de recurso: artículo
Fecha de publicación:2021
País:España
Institución:Universidad Miguel Hernández de Elche
Repositorio:REDIUMH. Depósito Digital de la UMH
OAI Identifier:oai:dspace.umh.es:11000/37902
Acceso en línea:https://hdl.handle.net/11000/37902
Access Level:acceso abierto
Palabra clave:antigen
COVID-19
Panbio
point-of-care
SARS-CoV-2
CDU::6 - Ciencias aplicadas::61 - Medicina
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spelling Nasopharyngeal Panbio COVID-19 Antigen Performed at Point-of-Care Has a High Sensitivity in Symptomatic and Asymptomatic Patients With Higher Risk for Transmission and Older AgeMasiá, MarFernández-González, MartaSánchez-Molla, ManuelCarvajal, MarGarcía, José AlbertoGonzalo Jiménez, NievesOrtiz de la Tabla, VictoriaAgulló, VanesaCandela, InmaculadaGuijarro, JorgeGutiérrez, José Antoniode Gregorio, CarlosGutiérrez, FélixantigenCOVID-19Panbiopoint-of-careSARS-CoV-2CDU::6 - Ciencias aplicadas::61 - MedicinaBackground. Performance of point-of-care tests in different clinical scenarios and on different samples remains undetermined. We comprehensively evaluated the performance of the nasopharyngeal Panbio COVID-19 Ag Rapid Test Device. Methods. This is a prospective study that includes consecutive patients attending 3 primary care centers (PCCs) and an emergency department. The antigen test was performed at point-of-care in nasopharyngeal and nasal swabs and in saliva. Positive percent agreement (PPA) and negative percent agreement (NPA) were calculated with the reverse-transcription polymerase chain reaction (RT-PCR) assay as reference standard. Results. Of 913 patients included, 296 (32.3%) were asymptomatic and 690 (75.6%) came from the PCC. Nasopharyngeal swabs were collected from 913 patients, nasal swabs were collected from 659 patients, and saliva was collected from 611 patients. The RT-PCR was positive in 196 (21.5%) nasopharyngeal samples (NPS). Overall, PPA (95% CI) in NPS was 60.5% (53.3–67.4), and it was lower in nasal swabs (44.7%) and saliva (23.1%). Test performance in NPS was largely dependent on the cycle threshold (Ct) in RT-PCR, with PPA of 94% for Ct ≤25 and 80% for Ct <30. In symptomatic patients, the PPA was 95% for Ct ≤25, 85% for Ct <30, and 89% for the symptom triad of fever, cough, and malaise. Performance was also dependent on age, with a PPA of 100% in symptomatic patients >50 years with Ct <25. In asymptomatic patients, the PPA was 86% for Ct <25. In all cases, NPA was 100%. Conclusions. The nasopharyngeal Panbio COVID-19 Ag test performed at point-of-care has a good sensitivity in symptomatic patients with Ct <30 and older age. The test was useful to identify asymptomatic patients with lower Ct values.Oxford University PressDepartamentos de la UMH::Medicina Clínica202520252021info:eu-repo/semantics/articleapplication/pdf9application/pdfhttps://hdl.handle.net/11000/37902reponame:REDIUMH. Depósito Digital de la UMHinstname:Universidad Miguel Hernández de ElcheIngléshttps://doi.org/10.1093/ofid/ofab059info:eu-repo/semantics/openAccessAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/oai:dspace.umh.es:11000/379022026-05-27T13:36:21Z
dc.title.none.fl_str_mv Nasopharyngeal Panbio COVID-19 Antigen Performed at Point-of-Care Has a High Sensitivity in Symptomatic and Asymptomatic Patients With Higher Risk for Transmission and Older Age
title Nasopharyngeal Panbio COVID-19 Antigen Performed at Point-of-Care Has a High Sensitivity in Symptomatic and Asymptomatic Patients With Higher Risk for Transmission and Older Age
spellingShingle Nasopharyngeal Panbio COVID-19 Antigen Performed at Point-of-Care Has a High Sensitivity in Symptomatic and Asymptomatic Patients With Higher Risk for Transmission and Older Age
Masiá, Mar
antigen
COVID-19
Panbio
point-of-care
SARS-CoV-2
CDU::6 - Ciencias aplicadas::61 - Medicina
title_short Nasopharyngeal Panbio COVID-19 Antigen Performed at Point-of-Care Has a High Sensitivity in Symptomatic and Asymptomatic Patients With Higher Risk for Transmission and Older Age
title_full Nasopharyngeal Panbio COVID-19 Antigen Performed at Point-of-Care Has a High Sensitivity in Symptomatic and Asymptomatic Patients With Higher Risk for Transmission and Older Age
title_fullStr Nasopharyngeal Panbio COVID-19 Antigen Performed at Point-of-Care Has a High Sensitivity in Symptomatic and Asymptomatic Patients With Higher Risk for Transmission and Older Age
title_full_unstemmed Nasopharyngeal Panbio COVID-19 Antigen Performed at Point-of-Care Has a High Sensitivity in Symptomatic and Asymptomatic Patients With Higher Risk for Transmission and Older Age
title_sort Nasopharyngeal Panbio COVID-19 Antigen Performed at Point-of-Care Has a High Sensitivity in Symptomatic and Asymptomatic Patients With Higher Risk for Transmission and Older Age
dc.creator.none.fl_str_mv Masiá, Mar
Fernández-González, Marta
Sánchez-Molla, Manuel
Carvajal, Mar
García, José Alberto
Gonzalo Jiménez, Nieves
Ortiz de la Tabla, Victoria
Agulló, Vanesa
Candela, Inmaculada
Guijarro, Jorge
Gutiérrez, José Antonio
de Gregorio, Carlos
Gutiérrez, Félix
author Masiá, Mar
author_facet Masiá, Mar
Fernández-González, Marta
Sánchez-Molla, Manuel
Carvajal, Mar
García, José Alberto
Gonzalo Jiménez, Nieves
Ortiz de la Tabla, Victoria
Agulló, Vanesa
Candela, Inmaculada
Guijarro, Jorge
Gutiérrez, José Antonio
de Gregorio, Carlos
Gutiérrez, Félix
author_role author
author2 Fernández-González, Marta
Sánchez-Molla, Manuel
Carvajal, Mar
García, José Alberto
Gonzalo Jiménez, Nieves
Ortiz de la Tabla, Victoria
Agulló, Vanesa
Candela, Inmaculada
Guijarro, Jorge
Gutiérrez, José Antonio
de Gregorio, Carlos
Gutiérrez, Félix
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Departamentos de la UMH::Medicina Clínica
dc.subject.none.fl_str_mv antigen
COVID-19
Panbio
point-of-care
SARS-CoV-2
CDU::6 - Ciencias aplicadas::61 - Medicina
topic antigen
COVID-19
Panbio
point-of-care
SARS-CoV-2
CDU::6 - Ciencias aplicadas::61 - Medicina
description Background. Performance of point-of-care tests in different clinical scenarios and on different samples remains undetermined. We comprehensively evaluated the performance of the nasopharyngeal Panbio COVID-19 Ag Rapid Test Device. Methods. This is a prospective study that includes consecutive patients attending 3 primary care centers (PCCs) and an emergency department. The antigen test was performed at point-of-care in nasopharyngeal and nasal swabs and in saliva. Positive percent agreement (PPA) and negative percent agreement (NPA) were calculated with the reverse-transcription polymerase chain reaction (RT-PCR) assay as reference standard. Results. Of 913 patients included, 296 (32.3%) were asymptomatic and 690 (75.6%) came from the PCC. Nasopharyngeal swabs were collected from 913 patients, nasal swabs were collected from 659 patients, and saliva was collected from 611 patients. The RT-PCR was positive in 196 (21.5%) nasopharyngeal samples (NPS). Overall, PPA (95% CI) in NPS was 60.5% (53.3–67.4), and it was lower in nasal swabs (44.7%) and saliva (23.1%). Test performance in NPS was largely dependent on the cycle threshold (Ct) in RT-PCR, with PPA of 94% for Ct ≤25 and 80% for Ct <30. In symptomatic patients, the PPA was 95% for Ct ≤25, 85% for Ct <30, and 89% for the symptom triad of fever, cough, and malaise. Performance was also dependent on age, with a PPA of 100% in symptomatic patients >50 years with Ct <25. In asymptomatic patients, the PPA was 86% for Ct <25. In all cases, NPA was 100%. Conclusions. The nasopharyngeal Panbio COVID-19 Ag test performed at point-of-care has a good sensitivity in symptomatic patients with Ct <30 and older age. The test was useful to identify asymptomatic patients with lower Ct values.
publishDate 2021
dc.date.none.fl_str_mv 2021
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/11000/37902
url https://hdl.handle.net/11000/37902
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.1093/ofid/ofab059
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.format.none.fl_str_mv application/pdf
9
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:REDIUMH. Depósito Digital de la UMH
instname:Universidad Miguel Hernández de Elche
instname_str Universidad Miguel Hernández de Elche
reponame_str REDIUMH. Depósito Digital de la UMH
collection REDIUMH. Depósito Digital de la UMH
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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