Monitoring Autophagic Flux in the Model Single-Celled Microalga Chlamydomonas reinhardtii

Autophagy is a catabolic process by which eukaryotic cells degrade and recycle unnecessary or damaged intracellular components to maintain cellular homeostasis and to cope with stress. The development of specific tools to monitor autophagy in microalgae and plants has been fundamental to investigate...

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Detalles Bibliográficos
Autores: Crespo, José L., Pérez-Pérez, María Esther
Tipo de recurso: artículo
Fecha de publicación:2023
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/355890
Acceso en línea:http://hdl.handle.net/10261/355890
Access Level:acceso abierto
Palabra clave:Autophagy
ATG8
Autophagic flux
Western blot
Chlamydomonas
Microalga
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spelling Monitoring Autophagic Flux in the Model Single-Celled Microalga Chlamydomonas reinhardtiiCrespo, José L.Pérez-Pérez, María EstherAutophagyATG8Autophagic fluxWestern blotChlamydomonasMicroalgaAutophagy is a catabolic process by which eukaryotic cells degrade and recycle unnecessary or damaged intracellular components to maintain cellular homeostasis and to cope with stress. The development of specific tools to monitor autophagy in microalgae and plants has been fundamental to investigate this catabolic pathway in photosynthetic organisms. The protein ATG8 is a widely used molecular marker of autophagy in all eukaryotes, including the model microalga Chlamydomonas reinhardtii. The drug concanamycin A, a specific inhibitor of vacuolar ATPase, has also been extensively used to block autophagic flux in the green lineage. In Chlamydomonas, inhibition of autophagic flux by concanamycin A has been shown to prevent the degradation of ribosomal proteins and the formation of lipid bodies under nitrogen or phosphorous starvation. Here, we detail how the abundance and lipidation state of ATG8 can be used to monitor autophagic flux in Chlamydomonas by western blot analysis.MEPP is supported by the Spanish Ministry of Science and Innovation (grant PID2019-110080GB-I00) and CSIC (grant 202040I006). JLC is supported by the Spanish Ministry of Science, Innovation and Universities (grant PGC2018-099048B-100) and the Regional Government of Andalusia (grant P20_00057).Peer reviewedSpringer NatureMinisterio de Ciencia, Innovación y Universidades (España)Agencia Estatal de Investigación (España)Junta de AndalucíaConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501application/pdfhttp://hdl.handle.net/10261/355890reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-110080GB-I00https://doi.org/10.1007/978-1-0716-2784-6_10Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3558902026-05-22T06:33:51Z
dc.title.none.fl_str_mv Monitoring Autophagic Flux in the Model Single-Celled Microalga Chlamydomonas reinhardtii
title Monitoring Autophagic Flux in the Model Single-Celled Microalga Chlamydomonas reinhardtii
spellingShingle Monitoring Autophagic Flux in the Model Single-Celled Microalga Chlamydomonas reinhardtii
Crespo, José L.
Autophagy
ATG8
Autophagic flux
Western blot
Chlamydomonas
Microalga
title_short Monitoring Autophagic Flux in the Model Single-Celled Microalga Chlamydomonas reinhardtii
title_full Monitoring Autophagic Flux in the Model Single-Celled Microalga Chlamydomonas reinhardtii
title_fullStr Monitoring Autophagic Flux in the Model Single-Celled Microalga Chlamydomonas reinhardtii
title_full_unstemmed Monitoring Autophagic Flux in the Model Single-Celled Microalga Chlamydomonas reinhardtii
title_sort Monitoring Autophagic Flux in the Model Single-Celled Microalga Chlamydomonas reinhardtii
dc.creator.none.fl_str_mv Crespo, José L.
Pérez-Pérez, María Esther
author Crespo, José L.
author_facet Crespo, José L.
Pérez-Pérez, María Esther
author_role author
author2 Pérez-Pérez, María Esther
author2_role author
dc.contributor.none.fl_str_mv Ministerio de Ciencia, Innovación y Universidades (España)
Agencia Estatal de Investigación (España)
Junta de Andalucía
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Autophagy
ATG8
Autophagic flux
Western blot
Chlamydomonas
Microalga
topic Autophagy
ATG8
Autophagic flux
Western blot
Chlamydomonas
Microalga
description Autophagy is a catabolic process by which eukaryotic cells degrade and recycle unnecessary or damaged intracellular components to maintain cellular homeostasis and to cope with stress. The development of specific tools to monitor autophagy in microalgae and plants has been fundamental to investigate this catabolic pathway in photosynthetic organisms. The protein ATG8 is a widely used molecular marker of autophagy in all eukaryotes, including the model microalga Chlamydomonas reinhardtii. The drug concanamycin A, a specific inhibitor of vacuolar ATPase, has also been extensively used to block autophagic flux in the green lineage. In Chlamydomonas, inhibition of autophagic flux by concanamycin A has been shown to prevent the degradation of ribosomal proteins and the formation of lipid bodies under nitrogen or phosphorous starvation. Here, we detail how the abundance and lipidation state of ATG8 can be used to monitor autophagic flux in Chlamydomonas by western blot analysis.
publishDate 2023
dc.date.none.fl_str_mv 2023
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/355890
url http://hdl.handle.net/10261/355890
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-110080GB-I00
https://doi.org/10.1007/978-1-0716-2784-6_10
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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