Leukocyte-endothelial cell interaction is enhanced in podocalyxin-deficient mice

16 p.-6 fig.

Detalles Bibliográficos
Autores: Porras, Gracia, Sánchez Ayuso, Matilde, González-Manchón, Consuelo
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2018
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/163140
Acceso en línea:http://hdl.handle.net/10261/163140
Access Level:acceso abierto
Palabra clave:Endothelial cell
Glycocalyx
Inflammation
Leukocyte-endothelial cell adhesion
Podocalyxin
id ES_d8fc3e739d00fedca5cc6b2cc2664abc
oai_identifier_str oai:digital.csic.es:10261/163140
network_acronym_str ES
network_name_str España
repository_id_str
spelling Leukocyte-endothelial cell interaction is enhanced in podocalyxin-deficient micePorras, GraciaSánchez Ayuso, MatildeGonzález-Manchón, ConsueloEndothelial cellGlycocalyxInflammationLeukocyte-endothelial cell adhesionPodocalyxin16 p.-6 fig.The highly sialoglycosylated extracellular domain of podocalyxin (Podxl) is a constituent of the endothelial glycocalyx of most blood vessels but it is unknown if Podxl plays a prominent role in the function of the glycocalyx as a regulator of leukocyte-endothelial adhesion. We have recently found that mice lacking Podxl in the vascular endothelium develop histological lesions compatible with severe vasculitis resulting in organ failure and premature death. In this work, we show that these mice have an increased quantity of resident leukocytes within the peritoneal cavity in both basal and inflammatory conditions. Adhesion of macrophagic cells to lung endothelial cells from Podxl-deficient mice was increased under inflammatory stimuli. Both, chemokine binding and chemokine-mediated adhesion of immune cells were significantly higher in Podxl-deficient endothelial cells. Moreover, glycocalyx function assessed by measuring the anticoagulant capacity of endothelial cell monolayers to inactivate thrombin was significantly altered in the absence of Podxl. Overall, the results suggest that Podxl is an essential component of the glycocalyx and has an important so far unknown role in preventing leukocyte-endothelial cell adhesion under resting and inflammatory conditions.This work was supported by grants SAF2007-61701 and BFU2010-15237 from the Secretaría de Estado de Investigación, Desarrollo e Innovación.Peer reviewedElsevierMinisterio de Economía y Competitividad (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201820182018info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/163140reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp:/dx.doi.org/10.1016/j.biocel.2018.03.018Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1631402026-05-22T06:33:51Z
dc.title.none.fl_str_mv Leukocyte-endothelial cell interaction is enhanced in podocalyxin-deficient mice
title Leukocyte-endothelial cell interaction is enhanced in podocalyxin-deficient mice
spellingShingle Leukocyte-endothelial cell interaction is enhanced in podocalyxin-deficient mice
Porras, Gracia
Endothelial cell
Glycocalyx
Inflammation
Leukocyte-endothelial cell adhesion
Podocalyxin
title_short Leukocyte-endothelial cell interaction is enhanced in podocalyxin-deficient mice
title_full Leukocyte-endothelial cell interaction is enhanced in podocalyxin-deficient mice
title_fullStr Leukocyte-endothelial cell interaction is enhanced in podocalyxin-deficient mice
title_full_unstemmed Leukocyte-endothelial cell interaction is enhanced in podocalyxin-deficient mice
title_sort Leukocyte-endothelial cell interaction is enhanced in podocalyxin-deficient mice
dc.creator.none.fl_str_mv Porras, Gracia
Sánchez Ayuso, Matilde
González-Manchón, Consuelo
author Porras, Gracia
author_facet Porras, Gracia
Sánchez Ayuso, Matilde
González-Manchón, Consuelo
author_role author
author2 Sánchez Ayuso, Matilde
González-Manchón, Consuelo
author2_role author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Endothelial cell
Glycocalyx
Inflammation
Leukocyte-endothelial cell adhesion
Podocalyxin
topic Endothelial cell
Glycocalyx
Inflammation
Leukocyte-endothelial cell adhesion
Podocalyxin
description 16 p.-6 fig.
publishDate 2018
dc.date.none.fl_str_mv 2018
2018
2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/163140
url http://hdl.handle.net/10261/163140
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http:/dx.doi.org/10.1016/j.biocel.2018.03.018
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869421250065989632
score 15,812429

Similares en LA Referencia