Reduced signal transduction by 5-HT4 receptors after long-term venlafaxine treatment in rats

El pdf del artículo es la versión pre-print.

Detalhes bibliográficos
Autores: Vidal, Rebeca, Valdizán, Elsa M., Vilaró, Maria Teresa, Pazos, Ángel, Castro, Elena
Tipo de documento: artigo
Data de publicação:2011
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositório:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/49376
Acesso em linha:http://hdl.handle.net/10261/49376
Access Level:Acceso aberto
Palavra-chave:Venlafaxine
In situ hybridization
Adenylate cyclase
Electrophysiology
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spelling Reduced signal transduction by 5-HT4 receptors after long-term venlafaxine treatment in ratsVidal, RebecaValdizán, Elsa M.Vilaró, Maria TeresaPazos, ÁngelCastro, ElenaVenlafaxineIn situ hybridizationAdenylate cyclaseElectrophysiologyEl pdf del artículo es la versión pre-print.[Background and Purpose]: The 5-HT4 receptor may be a target for antidepressant drugs. Here we have examined the effects of the dual antidepressant, venlafaxine, on 5-HT4 receptor-mediated signalling events. [Experimental Approach]: The effects of 21 days treatment (p.o.) with high (40 mg·kg−1) and low (10 mg·kg−1) doses of venlafaxine, were evaluated at different levels of 5-HT4 receptor-mediated neurotransmission by using in situ hybridization, receptor autoradiography, adenylate cyclase assays and electrophysiological recordings in rat brain. The selective noradrenaline reuptake inhibitor, reboxetine (10 mg·kg−1, 21 days) was also evaluated on 5-HT4 receptor density. [Key Results]: Treatment with a high dose (40 mg·kg−1) of venlafaxine did not alter 5-HT4 mRNA expression, but decreased the density of 5-HT4 receptors in caudate-putamen (% reduction = 26 ± 6), hippocampus (% reduction = 39 ± 7 and 39 ± 8 for CA1 and CA3 respectively) and substantia nigra (% reduction = 49 ± 5). Zacopride-stimulated adenylate cyclase activation was unaltered following low-dose treatment (10 mg·kg−1) while it was attenuated in rats treated with 40 mg·kg−1 of venlafaxine (% reduction = 51 ± 2). Furthermore, the amplitude of population spike in pyramidal cells of CA1 of hippocampus induced by zacopride was significantly attenuated in rats receiving either dose of venlafaxine. Chronic reboxetine did not modify 5-HT4 receptor density. [Conclusions and Implications]: Our data indicate a functional desensitization of 5-HT4 receptors after chronic venlafaxine, similar to that observed after treatment with the classical selective inhibitors of 5-HT reuptake.This research was supported by Ministry of Science, SAF04-00941, SAF07-61862, Fundación Alicia Koplowitz, Fundación de Investigación Médica Mutua Madrileña, Instituto de Salud Carlos III and University of Cantabria-FAES research contract. RV has been the recipient of a fellowship from University of Cantabria-FAES, and a CIBERSAM contract.Peer reviewedWiley-Blackwell201220122011info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/49376reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1111/j.1476-5381.2010.00903.xinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/493762026-05-22T06:33:51Z
dc.title.none.fl_str_mv Reduced signal transduction by 5-HT4 receptors after long-term venlafaxine treatment in rats
title Reduced signal transduction by 5-HT4 receptors after long-term venlafaxine treatment in rats
spellingShingle Reduced signal transduction by 5-HT4 receptors after long-term venlafaxine treatment in rats
Vidal, Rebeca
Venlafaxine
In situ hybridization
Adenylate cyclase
Electrophysiology
title_short Reduced signal transduction by 5-HT4 receptors after long-term venlafaxine treatment in rats
title_full Reduced signal transduction by 5-HT4 receptors after long-term venlafaxine treatment in rats
title_fullStr Reduced signal transduction by 5-HT4 receptors after long-term venlafaxine treatment in rats
title_full_unstemmed Reduced signal transduction by 5-HT4 receptors after long-term venlafaxine treatment in rats
title_sort Reduced signal transduction by 5-HT4 receptors after long-term venlafaxine treatment in rats
dc.creator.none.fl_str_mv Vidal, Rebeca
Valdizán, Elsa M.
Vilaró, Maria Teresa
Pazos, Ángel
Castro, Elena
author Vidal, Rebeca
author_facet Vidal, Rebeca
Valdizán, Elsa M.
Vilaró, Maria Teresa
Pazos, Ángel
Castro, Elena
author_role author
author2 Valdizán, Elsa M.
Vilaró, Maria Teresa
Pazos, Ángel
Castro, Elena
author2_role author
author
author
author
dc.subject.none.fl_str_mv Venlafaxine
In situ hybridization
Adenylate cyclase
Electrophysiology
topic Venlafaxine
In situ hybridization
Adenylate cyclase
Electrophysiology
description El pdf del artículo es la versión pre-print.
publishDate 2011
dc.date.none.fl_str_mv 2011
2012
2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/49376
url http://hdl.handle.net/10261/49376
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1111/j.1476-5381.2010.00903.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Wiley-Blackwell
publisher.none.fl_str_mv Wiley-Blackwell
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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