Association between the pig genome and its gut microbiota composition

The gut microbiota has been evolving with its host along the time creating a symbiotic relationship. In this study, we assess the role of the host genome in the modulation of the microbiota composition in pigs. Gut microbiota compositions were estimated through sequencing the V3-V4 region of the 16S...

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Detalles Bibliográficos
Autores: Crespo-Piazuelo, Daniel|||0000-0001-7896-2507, Migura-Garcia, Lourdes|||0000-0003-2935-928X, Estellé, Jordi|||0000-0002-6241-1732, Criado Mesas, Lourdes|||0000-0002-1115-4131, Revilla Sánchez, Manuel|||0000-0002-0164-9317, Castelló Farré, Anna|||0000-0001-8497-6251, Muñoz, María|||0000-0002-7018-6977, García Casco, Juan M., Fernández Ávila, Ana Isabel, Ballester Devis, Maria|||0000-0002-5413-4640, Folch, Josep M.|||0000-0003-3689-1303
Tipo de recurso: artículo
Fecha de publicación:2019
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:213823
Acceso en línea:https://ddd.uab.cat/record/213823
https://dx.doi.org/urn:doi:10.1038/s41598-019-45066-6
Access Level:acceso abierto
Palabra clave:Genetic markers
Genome-wide association studies
Metagenomics
Microbial ecology
Descripción
Sumario:The gut microbiota has been evolving with its host along the time creating a symbiotic relationship. In this study, we assess the role of the host genome in the modulation of the microbiota composition in pigs. Gut microbiota compositions were estimated through sequencing the V3-V4 region of the 16S rRNA gene from rectal contents of 285 pigs. A total of 1,261 operational taxonomic units were obtained and grouped in 18 phyla and 101 genera. Firmicutes (45.36%) and Bacteroidetes (37.47%) were the two major phyla obtained, whereas at genus level Prevotella (7.03%) and Treponema (6.29%) were the most abundant. Pigs were also genotyped with a high-throughput method for 45,508 single nucleotide polymorphisms that covered the entire pig genome. Subsequently, genome-wide association studies were made among the genotypes of these pigs and their gut microbiota composition. A total of 52 single-nucleotide polymorphisms distributed in 17 regions along the pig genome were associated with the relative abundance of six genera; Akkermansia, CF231, Phascolarctobacterium, Prevotella, SMB53, and Streptococcus. Our results suggest 39 candidate genes that may be modulating the microbiota composition and manifest the association between host genome and gut microbiota in pigs.

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