Engineering of Bacillus megaterium for improving PHA production from glycerol

9 p.-4 fig.

Detalles Bibliográficos
Autores: Gómez Cardozo, Javier Ricardo, Velasco-Bucheli, Rodrigo, Del Cerro, Carlos, Mata, Isabel de la, Mora Martínez, Amanda Lucía
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/189298
Acceso en línea:http://hdl.handle.net/10261/189298
Access Level:acceso abierto
Palabra clave:Bacillus megaterium
Glycerol
Glycerol dehydrogenase
Polyhydroxyalkanoates
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spelling Engineering of Bacillus megaterium for improving PHA production from glycerolGómez Cardozo, Javier RicardoVelasco-Bucheli, RodrigoDel Cerro, CarlosMata, Isabel de laMora Martínez, Amanda LucíaBacillus megateriumGlycerolGlycerol dehydrogenasePolyhydroxyalkanoates9 p.-4 fig.There are a few PHA-producer bacteria that can uptake glycerol to produce this biopolymer. Among them, Bacillus megaterium LVN01 has demonstrated to be able to grow up using glycerol as a carbon source. Glycerol dehydrogenase (GD) plays a key role in the synthesis of PHA from glycerol. In this study, the improvement of glycerol uptake by a recombinant strain of B. megaterium carrying pHT01-bmgd was evaluated in order to enhance PHA production. The biomass and PHA production were evaluated and compared to wild-type. It was determined that the PHA produced by both strains was PHB and the highest improvement in PHB yield was 226% at 30 h.This study was funded by Departamento Administrativo de Ciencia, Tecnología e Innovación de Colombia (COLCIENCIAS) (Called 647 of 2014). The authors would like to thank COLCIENCIAS for funding. This work is framed at the “Contrato de acceso a recursos genéticos y productos derivados del Ministerio del Medio Ambiente y Desarrollo Sostenible de Colombia Nº95 and Nº159”.Peer reviewedMalaysian Society of Molecular Biology & BiotechnologyColciencias (Colombia)Ministerio de Ambiente y Desarrollo Sostenible (Colombia)Gómez Cardozo, Javier Ricardo [0000-0001-5570-8194]Velasco-Bucheli, Rodrigo [0000-0002-1005-2475]Del Cerro, Carlos [0000-0003-1693-3895]De La Mata, I. [0000-0002-6637-2451]Mora Martínez, Amanda Lucía [0000-0002-3925-2278]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201920192019info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/189298reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.35118/apjmbb.2019.027.3.07Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1892982026-05-22T06:33:51Z
dc.title.none.fl_str_mv Engineering of Bacillus megaterium for improving PHA production from glycerol
title Engineering of Bacillus megaterium for improving PHA production from glycerol
spellingShingle Engineering of Bacillus megaterium for improving PHA production from glycerol
Gómez Cardozo, Javier Ricardo
Bacillus megaterium
Glycerol
Glycerol dehydrogenase
Polyhydroxyalkanoates
title_short Engineering of Bacillus megaterium for improving PHA production from glycerol
title_full Engineering of Bacillus megaterium for improving PHA production from glycerol
title_fullStr Engineering of Bacillus megaterium for improving PHA production from glycerol
title_full_unstemmed Engineering of Bacillus megaterium for improving PHA production from glycerol
title_sort Engineering of Bacillus megaterium for improving PHA production from glycerol
dc.creator.none.fl_str_mv Gómez Cardozo, Javier Ricardo
Velasco-Bucheli, Rodrigo
Del Cerro, Carlos
Mata, Isabel de la
Mora Martínez, Amanda Lucía
author Gómez Cardozo, Javier Ricardo
author_facet Gómez Cardozo, Javier Ricardo
Velasco-Bucheli, Rodrigo
Del Cerro, Carlos
Mata, Isabel de la
Mora Martínez, Amanda Lucía
author_role author
author2 Velasco-Bucheli, Rodrigo
Del Cerro, Carlos
Mata, Isabel de la
Mora Martínez, Amanda Lucía
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Colciencias (Colombia)
Ministerio de Ambiente y Desarrollo Sostenible (Colombia)
Gómez Cardozo, Javier Ricardo [0000-0001-5570-8194]
Velasco-Bucheli, Rodrigo [0000-0002-1005-2475]
Del Cerro, Carlos [0000-0003-1693-3895]
De La Mata, I. [0000-0002-6637-2451]
Mora Martínez, Amanda Lucía [0000-0002-3925-2278]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Bacillus megaterium
Glycerol
Glycerol dehydrogenase
Polyhydroxyalkanoates
topic Bacillus megaterium
Glycerol
Glycerol dehydrogenase
Polyhydroxyalkanoates
description 9 p.-4 fig.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/189298
url http://hdl.handle.net/10261/189298
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.35118/apjmbb.2019.027.3.07
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Malaysian Society of Molecular Biology & Biotechnology
publisher.none.fl_str_mv Malaysian Society of Molecular Biology & Biotechnology
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869421153613774848
score 15,811543

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