Grape epicatechin conjugates prevent erythrocyte membrane protein oxidation

Epicatechin conjugates obtained from grape have shown antioxidant activity in various systems. However, how these conjugates exert their antioxidant benefits has not been widely studied. We assessed the activity of epicatechin and epicatechin conjugates on the erythrocyte membrane in the presence an...

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Authors: Martínez Ocaña, Verónica, Ugartondo Casadevall, Vanessa, Vinardell Martínez-Hidalgo, Ma. Pilar, Torres Simón, Josep Lluís, Mitjans Arnal, Montserrat
Format: article
Status:Versión aceptada para publicación
Publication Date:2012
Country:España
Institution:Universidad de Barcelona
Repository:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/32319
Online Access:https://hdl.handle.net/2445/32319
Access Level:Open access
Keyword:Polifenols
Antioxidants
Raïms
Hematies
Proteïnes de membrana
Polyphenols
Grapes
Erythrocytes
Membrane proteins
id ES_d83a4be7cd5f60d5cfbe79e0bb29ada0
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spelling Grape epicatechin conjugates prevent erythrocyte membrane protein oxidationMartínez Ocaña, VerónicaUgartondo Casadevall, VanessaVinardell Martínez-Hidalgo, Ma. PilarTorres Simón, Josep LluísMitjans Arnal, MontserratPolifenolsAntioxidantsRaïmsHematiesProteïnes de membranaPolyphenolsAntioxidantsGrapesErythrocytesMembrane proteinsEpicatechin conjugates obtained from grape have shown antioxidant activity in various systems. However, how these conjugates exert their antioxidant benefits has not been widely studied. We assessed the activity of epicatechin and epicatechin conjugates on the erythrocyte membrane in the presence and absence of a peroxyl radical initiator, to increase our understanding of their mechanisms. Thus, we studied cell membrane fluidity by fluorescence anisotropy measurements, morphology of erythrocytes by scanning electron microscopy, and finally, red cell membrane proteins by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Our data showed that incubation of red cells in the presence of epicatechin derivatives altered membrane fluidity and erythrocyte morphology but not the membrane protein pattern. The presence in the medium of the peroxyl radical initiator 2,2′-azobis(amidinopropane) dihydrochloride (AAPH) resulted in membrane disruptions at all levels analyzed, causing changes in membrane fluidity, cell morphology, and protein degradation. The presence of antioxidants avoided protein oxidation, indicating that the interaction of epicatechin conjugates with the lipid bilayer might reduce the accessibility of AAPH to membranes, which could explain in part the inhibitory ability of these compounds against hemolysis induced by peroxidative insult.American Chemical Society, Books and Journals Division2012info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttps://hdl.handle.net/2445/32319Articles publicats en revistes (Bioquímica i Fisiologia)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésVersió postprint del document publicat a: http://dx.doi.org/10.1021/jf2051784Journal of Agricultural and Food Chemistry, 2012, vol. 60, num. 16, p. 4090-4095http://dx.doi.org/10.1021/jf2051784(c) American Chemical Society, Books and Journals Division], 2012info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/323192026-05-27T06:46:51Z
dc.title.none.fl_str_mv Grape epicatechin conjugates prevent erythrocyte membrane protein oxidation
title Grape epicatechin conjugates prevent erythrocyte membrane protein oxidation
spellingShingle Grape epicatechin conjugates prevent erythrocyte membrane protein oxidation
Martínez Ocaña, Verónica
Polifenols
Antioxidants
Raïms
Hematies
Proteïnes de membrana
Polyphenols
Antioxidants
Grapes
Erythrocytes
Membrane proteins
title_short Grape epicatechin conjugates prevent erythrocyte membrane protein oxidation
title_full Grape epicatechin conjugates prevent erythrocyte membrane protein oxidation
title_fullStr Grape epicatechin conjugates prevent erythrocyte membrane protein oxidation
title_full_unstemmed Grape epicatechin conjugates prevent erythrocyte membrane protein oxidation
title_sort Grape epicatechin conjugates prevent erythrocyte membrane protein oxidation
dc.creator.none.fl_str_mv Martínez Ocaña, Verónica
Ugartondo Casadevall, Vanessa
Vinardell Martínez-Hidalgo, Ma. Pilar
Torres Simón, Josep Lluís
Mitjans Arnal, Montserrat
author Martínez Ocaña, Verónica
author_facet Martínez Ocaña, Verónica
Ugartondo Casadevall, Vanessa
Vinardell Martínez-Hidalgo, Ma. Pilar
Torres Simón, Josep Lluís
Mitjans Arnal, Montserrat
author_role author
author2 Ugartondo Casadevall, Vanessa
Vinardell Martínez-Hidalgo, Ma. Pilar
Torres Simón, Josep Lluís
Mitjans Arnal, Montserrat
author2_role author
author
author
author
dc.subject.none.fl_str_mv Polifenols
Antioxidants
Raïms
Hematies
Proteïnes de membrana
Polyphenols
Antioxidants
Grapes
Erythrocytes
Membrane proteins
topic Polifenols
Antioxidants
Raïms
Hematies
Proteïnes de membrana
Polyphenols
Antioxidants
Grapes
Erythrocytes
Membrane proteins
description Epicatechin conjugates obtained from grape have shown antioxidant activity in various systems. However, how these conjugates exert their antioxidant benefits has not been widely studied. We assessed the activity of epicatechin and epicatechin conjugates on the erythrocyte membrane in the presence and absence of a peroxyl radical initiator, to increase our understanding of their mechanisms. Thus, we studied cell membrane fluidity by fluorescence anisotropy measurements, morphology of erythrocytes by scanning electron microscopy, and finally, red cell membrane proteins by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Our data showed that incubation of red cells in the presence of epicatechin derivatives altered membrane fluidity and erythrocyte morphology but not the membrane protein pattern. The presence in the medium of the peroxyl radical initiator 2,2′-azobis(amidinopropane) dihydrochloride (AAPH) resulted in membrane disruptions at all levels analyzed, causing changes in membrane fluidity, cell morphology, and protein degradation. The presence of antioxidants avoided protein oxidation, indicating that the interaction of epicatechin conjugates with the lipid bilayer might reduce the accessibility of AAPH to membranes, which could explain in part the inhibitory ability of these compounds against hemolysis induced by peroxidative insult.
publishDate 2012
dc.date.none.fl_str_mv 2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/32319
url https://hdl.handle.net/2445/32319
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Versió postprint del document publicat a: http://dx.doi.org/10.1021/jf2051784
Journal of Agricultural and Food Chemistry, 2012, vol. 60, num. 16, p. 4090-4095
http://dx.doi.org/10.1021/jf2051784
dc.rights.none.fl_str_mv (c) American Chemical Society, Books and Journals Division], 2012
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) American Chemical Society, Books and Journals Division], 2012
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Chemical Society, Books and Journals Division
publisher.none.fl_str_mv American Chemical Society, Books and Journals Division
dc.source.none.fl_str_mv Articles publicats en revistes (Bioquímica i Fisiologia)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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