The THP1-SAC3-SUS1-CDC31 complex works in transcription elongation-mRNA export preventing RNA-mediated genome instability

The eukaryotic THO/TREX complex, involved in mRNP biogenesis, plays a key role in the maintenance of genome integrity in yeast. mRNA export factors such as Thp1-Sac3 also affect genome integrity, but their mutations have other phenotypes different from those of THO/TREX. Sus1 is a novel component of...

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Detalles Bibliográficos
Autores: González Aguilera, Cristina, Tous Rivera, Cristina, Gómez González, Belén, Huertas Sánchez, Pablo, Luna Varo, Rosa María, Aguilera López, Andrés
Tipo de recurso: artículo
Fecha de publicación:2008
País:España
Institución:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/29169
Acceso en línea:http://hdl.handle.net/11441/29169
https://doi.org/10.1091/mbc.E08-04-0355
Access Level:acceso abierto
Palabra clave:Cell Cycle Proteins/metabolism
Genomic Instability
Nuclear Proteins/metabolism
RNA, Messenger/metabolism
RNA-Binding Proteins/metabolism
Ribonucleoproteins/metabolism
Saccharomyces cerevisiae/physiology
Saccharomyces cerevisiae Proteins/metabolism
Transcription, Genetic
Calcium-Binding Proteins
metabolism
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spelling The THP1-SAC3-SUS1-CDC31 complex works in transcription elongation-mRNA export preventing RNA-mediated genome instabilityGonzález Aguilera, CristinaTous Rivera, CristinaGómez González, BelénHuertas Sánchez, PabloLuna Varo, Rosa MaríaAguilera López, AndrésCell Cycle Proteins/metabolismGenomic InstabilityNuclear Proteins/metabolismRNA, Messenger/metabolismRNA-Binding Proteins/metabolismRibonucleoproteins/metabolismSaccharomyces cerevisiae/physiologySaccharomyces cerevisiae Proteins/metabolismTranscription, GeneticCalcium-Binding ProteinsmetabolismThe eukaryotic THO/TREX complex, involved in mRNP biogenesis, plays a key role in the maintenance of genome integrity in yeast. mRNA export factors such as Thp1-Sac3 also affect genome integrity, but their mutations have other phenotypes different from those of THO/TREX. Sus1 is a novel component of SAGA transcription factor that also associates with Thp1-Sac3, but little is known about its effect on genome instability and transcription. Here we show that Thp1, Sac3, and Sus1 form a functional unit with a role in mRNP biogenesis and maintenance of genome integrity that is independent of SAGA. Importantly, the effects of ribozyme-containing transcription units, RNase H, and the action of human activation-induced cytidine deaminase on transcription and genome instability are consistent with the possibility that R-loops are formed in Thp1-Sac3-Sus1-Cdc31 as in THO mutants. Our data reveal that Thp1-Sac3-Sus1-Cdc31, together with THO/TREX, define a specific pathway connecting transcription elongation with export via an RNA-dependent dynamic process that provides a feedback mechanism for the control of transcription and the preservation of genetic integrity of transcribed DNA regions.American Society for Cell BiologyGenética2008info:eu-repo/semantics/articleapplication/pdfapplication/pdfhttp://hdl.handle.net/11441/29169https://doi.org/10.1091/mbc.E08-04-0355reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésMolecular Biology of the Cell, 19(10), 4310-4318http://dx.doi.org/10.1091/mbc.E08-04-0355info:eu-repo/semantics/openAccessoai:idus.us.es:11441/291692026-06-17T12:51:07Z
dc.title.none.fl_str_mv The THP1-SAC3-SUS1-CDC31 complex works in transcription elongation-mRNA export preventing RNA-mediated genome instability
title The THP1-SAC3-SUS1-CDC31 complex works in transcription elongation-mRNA export preventing RNA-mediated genome instability
spellingShingle The THP1-SAC3-SUS1-CDC31 complex works in transcription elongation-mRNA export preventing RNA-mediated genome instability
González Aguilera, Cristina
Cell Cycle Proteins/metabolism
Genomic Instability
Nuclear Proteins/metabolism
RNA, Messenger/metabolism
RNA-Binding Proteins/metabolism
Ribonucleoproteins/metabolism
Saccharomyces cerevisiae/physiology
Saccharomyces cerevisiae Proteins/metabolism
Transcription, Genetic
Calcium-Binding Proteins
metabolism
title_short The THP1-SAC3-SUS1-CDC31 complex works in transcription elongation-mRNA export preventing RNA-mediated genome instability
title_full The THP1-SAC3-SUS1-CDC31 complex works in transcription elongation-mRNA export preventing RNA-mediated genome instability
title_fullStr The THP1-SAC3-SUS1-CDC31 complex works in transcription elongation-mRNA export preventing RNA-mediated genome instability
title_full_unstemmed The THP1-SAC3-SUS1-CDC31 complex works in transcription elongation-mRNA export preventing RNA-mediated genome instability
title_sort The THP1-SAC3-SUS1-CDC31 complex works in transcription elongation-mRNA export preventing RNA-mediated genome instability
dc.creator.none.fl_str_mv González Aguilera, Cristina
Tous Rivera, Cristina
Gómez González, Belén
Huertas Sánchez, Pablo
Luna Varo, Rosa María
Aguilera López, Andrés
author González Aguilera, Cristina
author_facet González Aguilera, Cristina
Tous Rivera, Cristina
Gómez González, Belén
Huertas Sánchez, Pablo
Luna Varo, Rosa María
Aguilera López, Andrés
author_role author
author2 Tous Rivera, Cristina
Gómez González, Belén
Huertas Sánchez, Pablo
Luna Varo, Rosa María
Aguilera López, Andrés
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Genética
dc.subject.none.fl_str_mv Cell Cycle Proteins/metabolism
Genomic Instability
Nuclear Proteins/metabolism
RNA, Messenger/metabolism
RNA-Binding Proteins/metabolism
Ribonucleoproteins/metabolism
Saccharomyces cerevisiae/physiology
Saccharomyces cerevisiae Proteins/metabolism
Transcription, Genetic
Calcium-Binding Proteins
metabolism
topic Cell Cycle Proteins/metabolism
Genomic Instability
Nuclear Proteins/metabolism
RNA, Messenger/metabolism
RNA-Binding Proteins/metabolism
Ribonucleoproteins/metabolism
Saccharomyces cerevisiae/physiology
Saccharomyces cerevisiae Proteins/metabolism
Transcription, Genetic
Calcium-Binding Proteins
metabolism
description The eukaryotic THO/TREX complex, involved in mRNP biogenesis, plays a key role in the maintenance of genome integrity in yeast. mRNA export factors such as Thp1-Sac3 also affect genome integrity, but their mutations have other phenotypes different from those of THO/TREX. Sus1 is a novel component of SAGA transcription factor that also associates with Thp1-Sac3, but little is known about its effect on genome instability and transcription. Here we show that Thp1, Sac3, and Sus1 form a functional unit with a role in mRNP biogenesis and maintenance of genome integrity that is independent of SAGA. Importantly, the effects of ribozyme-containing transcription units, RNase H, and the action of human activation-induced cytidine deaminase on transcription and genome instability are consistent with the possibility that R-loops are formed in Thp1-Sac3-Sus1-Cdc31 as in THO mutants. Our data reveal that Thp1-Sac3-Sus1-Cdc31, together with THO/TREX, define a specific pathway connecting transcription elongation with export via an RNA-dependent dynamic process that provides a feedback mechanism for the control of transcription and the preservation of genetic integrity of transcribed DNA regions.
publishDate 2008
dc.date.none.fl_str_mv 2008
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/11441/29169
https://doi.org/10.1091/mbc.E08-04-0355
url http://hdl.handle.net/11441/29169
https://doi.org/10.1091/mbc.E08-04-0355
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Molecular Biology of the Cell, 19(10), 4310-4318
http://dx.doi.org/10.1091/mbc.E08-04-0355
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society for Cell Biology
publisher.none.fl_str_mv American Society for Cell Biology
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.300719

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