H3K4 monomethylation dictates nucleosome dynamics and chromatin remodeling at stress-responsive genes

Chromatin remodeling is essential for proper adaptation to extracellular stimuli. The p38-related Hog1 SAPK is an important regulator of transcription that mediates chromatin remodeling upon stress. Hog1 targets the RSC chromatin remodeling complex to stress-responsive genes and rsc deficient cells...

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Autores: Nadal Jiménez, Mariona, Mas, Glòria, Millán Zambrano, Gonzalo, Solé, Carme, Chávez de Diego, Sebastián, Nadal, Eulàlia de
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2015
País:España
Recursos:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/55827
Acesso em linha:http://hdl.handle.net/11441/55827
https://doi.org/10.1093/nar/gkv220
Access Level:acceso abierto
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spelling H3K4 monomethylation dictates nucleosome dynamics and chromatin remodeling at stress-responsive genesNadal Jiménez, MarionaMas, GlòriaMillán Zambrano, GonzaloSolé, CarmeChávez de Diego, SebastiánNadal, Eulàlia deChromatin remodeling is essential for proper adaptation to extracellular stimuli. The p38-related Hog1 SAPK is an important regulator of transcription that mediates chromatin remodeling upon stress. Hog1 targets the RSC chromatin remodeling complex to stress-responsive genes and rsc deficient cells display reduced induction of gene expression. Here we show that the absence of H3K4 methylation, either achieved by deletion of the SET1 methyltransferase or by amino acid substitution of H3K4, bypasses the requirement of RSC for stress-responsive gene expression. Monomethylation of H3K4 is specifically inhibiting RSC-independent chromatin remodeling and thus, it prevents osmostress-induced gene expression. The absence of H3K4 monomethylation permits that the association of alternative remodelers with stress-responsive genes and the Swr1 complex (SWR-C) is instrumental in the induction of gene expression upon stress. Accordingly, the absence of SWR-C or histone H2A.Z results in compromised chromatin remodeling and impaired gene expression in the absence of RSC and H3K4 methylation. These results indicate that expression of stress-responsive genes is controlled by two remodeling mechanisms: RSC in the presence of monomethylated H3K4, and SWR-C in the absence of H3K4 monomethylation. Our findings point to a novel role for H3K4 monomethylation in dictating the specificity of chromatin remodeling, adding an extra layer of regulation to the transcriptional stress response.España Ministerio de Economía y Competitividad BFU2012-33503España Ministerio de Economía y Competitividad BFU2013-48643-C3-1-PJunta de Andalucía BIO-271Junta de Andalucía P12-BIO-193España Ministerio de Economía y Competitividad BFU2011-26722Oxford University PressGenética2015info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/11441/55827https://doi.org/10.1093/nar/gkv220reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésNucleic Acids Research, 43 (10), 4937-4949.BFU2013-48643-C3-1-PBFU2012-33503BIO-271P12-BIO-193BFU2011-26722http://dx.doi.org/10.1093/nar/gkv220info:eu-repo/semantics/openAccessoai:idus.us.es:11441/558272026-06-17T12:51:07Z
dc.title.none.fl_str_mv H3K4 monomethylation dictates nucleosome dynamics and chromatin remodeling at stress-responsive genes
title H3K4 monomethylation dictates nucleosome dynamics and chromatin remodeling at stress-responsive genes
spellingShingle H3K4 monomethylation dictates nucleosome dynamics and chromatin remodeling at stress-responsive genes
Nadal Jiménez, Mariona
title_short H3K4 monomethylation dictates nucleosome dynamics and chromatin remodeling at stress-responsive genes
title_full H3K4 monomethylation dictates nucleosome dynamics and chromatin remodeling at stress-responsive genes
title_fullStr H3K4 monomethylation dictates nucleosome dynamics and chromatin remodeling at stress-responsive genes
title_full_unstemmed H3K4 monomethylation dictates nucleosome dynamics and chromatin remodeling at stress-responsive genes
title_sort H3K4 monomethylation dictates nucleosome dynamics and chromatin remodeling at stress-responsive genes
dc.creator.none.fl_str_mv Nadal Jiménez, Mariona
Mas, Glòria
Millán Zambrano, Gonzalo
Solé, Carme
Chávez de Diego, Sebastián
Nadal, Eulàlia de
author Nadal Jiménez, Mariona
author_facet Nadal Jiménez, Mariona
Mas, Glòria
Millán Zambrano, Gonzalo
Solé, Carme
Chávez de Diego, Sebastián
Nadal, Eulàlia de
author_role author
author2 Mas, Glòria
Millán Zambrano, Gonzalo
Solé, Carme
Chávez de Diego, Sebastián
Nadal, Eulàlia de
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Genética
description Chromatin remodeling is essential for proper adaptation to extracellular stimuli. The p38-related Hog1 SAPK is an important regulator of transcription that mediates chromatin remodeling upon stress. Hog1 targets the RSC chromatin remodeling complex to stress-responsive genes and rsc deficient cells display reduced induction of gene expression. Here we show that the absence of H3K4 methylation, either achieved by deletion of the SET1 methyltransferase or by amino acid substitution of H3K4, bypasses the requirement of RSC for stress-responsive gene expression. Monomethylation of H3K4 is specifically inhibiting RSC-independent chromatin remodeling and thus, it prevents osmostress-induced gene expression. The absence of H3K4 monomethylation permits that the association of alternative remodelers with stress-responsive genes and the Swr1 complex (SWR-C) is instrumental in the induction of gene expression upon stress. Accordingly, the absence of SWR-C or histone H2A.Z results in compromised chromatin remodeling and impaired gene expression in the absence of RSC and H3K4 methylation. These results indicate that expression of stress-responsive genes is controlled by two remodeling mechanisms: RSC in the presence of monomethylated H3K4, and SWR-C in the absence of H3K4 monomethylation. Our findings point to a novel role for H3K4 monomethylation in dictating the specificity of chromatin remodeling, adding an extra layer of regulation to the transcriptional stress response.
publishDate 2015
dc.date.none.fl_str_mv 2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11441/55827
https://doi.org/10.1093/nar/gkv220
url http://hdl.handle.net/11441/55827
https://doi.org/10.1093/nar/gkv220
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Nucleic Acids Research, 43 (10), 4937-4949.
BFU2013-48643-C3-1-P
BFU2012-33503
BIO-271
P12-BIO-193
BFU2011-26722
http://dx.doi.org/10.1093/nar/gkv220
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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