A multi-OMIC characterisation of biodegradation and microbial community succession within the PET plastisphere

[Background] Plastics now pollute marine environments across the globe. On entering these environments, plastics are rapidly colonised by a diverse community of microorganisms termed the plastisphere. Members of the plastisphere have a myriad of diverse functions typically found in any biofilm but,...

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Autores: Wright, Robyn J., Bosch, Rafael, Langille, Morgan G. I., Gibson, Matthew I., Christie-Oleza, Joseph Alexander
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/257483
Acceso en línea:http://hdl.handle.net/10261/257483
Access Level:acceso abierto
Palabra clave:Plastisphere
Polyethylene terephthalate
Plastic biodegradation
Microbial community succession
Proteogenomics
Metabolomics
id ES_d7e7f343737cef45b1d87757bb8a4fe4
oai_identifier_str oai:digital.csic.es:10261/257483
network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv A multi-OMIC characterisation of biodegradation and microbial community succession within the PET plastisphere
title A multi-OMIC characterisation of biodegradation and microbial community succession within the PET plastisphere
spellingShingle A multi-OMIC characterisation of biodegradation and microbial community succession within the PET plastisphere
Wright, Robyn J.
Plastisphere
Polyethylene terephthalate
Plastic biodegradation
Microbial community succession
Proteogenomics
Metabolomics
title_short A multi-OMIC characterisation of biodegradation and microbial community succession within the PET plastisphere
title_full A multi-OMIC characterisation of biodegradation and microbial community succession within the PET plastisphere
title_fullStr A multi-OMIC characterisation of biodegradation and microbial community succession within the PET plastisphere
title_full_unstemmed A multi-OMIC characterisation of biodegradation and microbial community succession within the PET plastisphere
title_sort A multi-OMIC characterisation of biodegradation and microbial community succession within the PET plastisphere
dc.creator.none.fl_str_mv Wright, Robyn J.
Bosch, Rafael
Langille, Morgan G. I.
Gibson, Matthew I.
Christie-Oleza, Joseph Alexander
author Wright, Robyn J.
author_facet Wright, Robyn J.
Bosch, Rafael
Langille, Morgan G. I.
Gibson, Matthew I.
Christie-Oleza, Joseph Alexander
author_role author
author2 Bosch, Rafael
Langille, Morgan G. I.
Gibson, Matthew I.
Christie-Oleza, Joseph Alexander
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Natural Environment Research Council (UK)
Ministerio de Ciencia, Innovación y Universidades (España)
Agencia Estatal de Investigación (España)
European Commission
Ministerio de Economía y Competitividad (España)
European Research Council
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Plastisphere
Polyethylene terephthalate
Plastic biodegradation
Microbial community succession
Proteogenomics
Metabolomics
topic Plastisphere
Polyethylene terephthalate
Plastic biodegradation
Microbial community succession
Proteogenomics
Metabolomics
description [Background] Plastics now pollute marine environments across the globe. On entering these environments, plastics are rapidly colonised by a diverse community of microorganisms termed the plastisphere. Members of the plastisphere have a myriad of diverse functions typically found in any biofilm but, additionally, a number of marine plastisphere studies have claimed the presence of plastic-biodegrading organisms, although with little mechanistic verification. Here, we obtained a microbial community from marine plastic debris and analysed the community succession across 6 weeks of incubation with different polyethylene terephthalate (PET) products as the sole carbon source, and further characterised the mechanisms involved in PET degradation by two bacterial isolates from the plastisphere.
publishDate 2021
dc.date.none.fl_str_mv 2021
2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/257483
url http://hdl.handle.net/10261/257483
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
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#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MICINN//RYC-2017-22452
info:eu-repo/grantAgreement/MINECO//CTM2015-70180-R
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-109509RB-I00
info:eu-repo/grantAgreement/EC/H2020/638631
Wright, Robyn J.; Bosch, Rafael; Langille, Morgan G. I.; Gibson, Matthew I.; Christie-Oleza, Joseph Alexander. Correction to: A multi-OMIC characterisation of biodegradation and microbial community succession within the PET plastisphere. Microbiome 9: 155 (2021). https://doi.org/10.1186/s40168-021-01120-y . http://hdl.handle.net/10261/257498
http://dx.doi.org/10.1186/s40168-021-01054-5
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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spelling A multi-OMIC characterisation of biodegradation and microbial community succession within the PET plastisphereWright, Robyn J.Bosch, RafaelLangille, Morgan G. I.Gibson, Matthew I.Christie-Oleza, Joseph AlexanderPlastispherePolyethylene terephthalatePlastic biodegradationMicrobial community successionProteogenomicsMetabolomics[Background] Plastics now pollute marine environments across the globe. On entering these environments, plastics are rapidly colonised by a diverse community of microorganisms termed the plastisphere. Members of the plastisphere have a myriad of diverse functions typically found in any biofilm but, additionally, a number of marine plastisphere studies have claimed the presence of plastic-biodegrading organisms, although with little mechanistic verification. Here, we obtained a microbial community from marine plastic debris and analysed the community succession across 6 weeks of incubation with different polyethylene terephthalate (PET) products as the sole carbon source, and further characterised the mechanisms involved in PET degradation by two bacterial isolates from the plastisphere.[Results] We found that all communities differed significantly from the inoculum and were dominated by Gammaproteobacteria, i.e. Alteromonadaceae and Thalassospiraceae at early time points, Alcanivoraceae at later time points and Vibrionaceae throughout. The large number of encoded enzymes involved in PET degradation found in predicted metagenomes and the observation of polymer oxidation by FTIR analyses both suggested PET degradation was occurring. However, we were unable to detect intermediates of PET hydrolysis with metabolomic analyses, which may be attributed to their rapid depletion by the complex community. To further confirm the PET biodegrading potential within the plastisphere of marine plastic debris, we used a combined proteogenomic and metabolomic approach to characterise amorphous PET degradation by two novel marine isolates, Thioclava sp. BHET1 and Bacillus sp. BHET2. The identification of PET hydrolytic intermediates by metabolomics confirmed that both isolates were able to degrade PET. High-throughput proteomics revealed that whilst Thioclava sp. BHET1 used the degradation pathway identified in terrestrial environment counterparts, these were absent in Bacillus sp. BHET2, indicating that either the enzymes used by this bacterium share little homology with those characterised previously, or that this bacterium uses a novel pathway for PET degradation.[Conclusions] Overall, the results of our multi-OMIC characterisation of PET degradation provide a significant step forwards in our understanding of marine plastic degradation by bacterial isolates and communities and evidences the biodegrading potential extant in the plastisphere of marine plastic debris.RW was supported by a Midlands Integrative Biosciences Training Partnership PhD scholarship via grant BB/M01116X/1, JC-O by the Natural Environment Research Council Independent Research Fellowship NE/K009044/1 and Ramón y Cajal contract RYC-2017-22452 (funded by the Ministry of Science, Innovation and Universities, the National Agency of Research, and the European Social Fund), RB by the MINECO project CTM2015-70180-R (FEDER cofunding) and MIG by European Research Council grant 638631. This work was supported by the Agencia Estatal de Investigación (project PID2019-109509RB-I00 / AEI / 10.13039/501100011033).BioMed CentralNatural Environment Research Council (UK)Ministerio de Ciencia, Innovación y Universidades (España)Agencia Estatal de Investigación (España)European CommissionMinisterio de Economía y Competitividad (España)European Research CouncilConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2022202220212022info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/257483reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MICINN//RYC-2017-22452info:eu-repo/grantAgreement/MINECO//CTM2015-70180-Rinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-109509RB-I00info:eu-repo/grantAgreement/EC/H2020/638631Wright, Robyn J.; Bosch, Rafael; Langille, Morgan G. I.; Gibson, Matthew I.; Christie-Oleza, Joseph Alexander. Correction to: A multi-OMIC characterisation of biodegradation and microbial community succession within the PET plastisphere. Microbiome 9: 155 (2021). https://doi.org/10.1186/s40168-021-01120-y . http://hdl.handle.net/10261/257498http://dx.doi.org/10.1186/s40168-021-01054-5Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2574832026-05-22T06:33:51Z
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