Comprehensive RNA-Seq profiling to evaluate lactating sheep mammary gland transcriptome

[EN] RNA-Seq enables the generation of extensive transcriptome information providing the capability to characterize transcripts (including alternative isoforms and polymorphism), to quantify expression and to identify differential regulation in a single experiment. Our aim in this study was to take...

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Autores: Suárez Vega, Aroa, Gutiérrez Gil, Beatriz, Klopp, Christophe, Tosser-Klopp, Gwenola, Arranz Santos, Juan José
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:España
Recursos:Universidad de León
Repositorio:BULERIA. Repositorio Institucional de la Universidad de León
OAI Identifier:oai:buleria.unileon.es:10612/24342
Acesso em linha:https://www.nature.com/articles/sdata201651
https://hdl.handle.net/10612/24342
Access Level:acceso abierto
Palavra-chave:Genética
Producción animal
Veterinaria
Time series design
Strain comparison design
Transcription profiling design
Transcription profiling assay
RNA sequencing
Selectively maintained organism
Time measurement datum
Ovis aries
Milk
3109 Ciencias Veterinarias
3104 Producción Animal
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oai_identifier_str oai:buleria.unileon.es:10612/24342
network_acronym_str ES
network_name_str España
repository_id_str
spelling Comprehensive RNA-Seq profiling to evaluate lactating sheep mammary gland transcriptomeSuárez Vega, AroaGutiérrez Gil, BeatrizKlopp, ChristopheTosser-Klopp, GwenolaArranz Santos, Juan JoséGenéticaProducción animalVeterinariaTime series designStrain comparison designTranscription profiling designTranscription profiling assayRNA sequencingSelectively maintained organismTime measurement datumOvis ariesMilk3109 Ciencias Veterinarias3104 Producción Animal[EN] RNA-Seq enables the generation of extensive transcriptome information providing the capability to characterize transcripts (including alternative isoforms and polymorphism), to quantify expression and to identify differential regulation in a single experiment. Our aim in this study was to take advantage of using RNA-Seq high-throughput technology to provide a comprehensive transcriptome profiling of the sheep lactating mammary gland. Eight ewes of two dairy sheep breeds with differences in milk production traits were used in this experiment (four Churra and four Assaf ewes). Milk samples from these animals were collected on days 10, 50, 120 and 150 after lambing to cover the various physiological stages of the mammary gland across the complete lactation. RNA samples were extracted from milk somatic cells. The RNA-Seq dataset was generated using an Illumina HiSeq 2000 sequencer. The information reported here will be useful to understand the biology of lactation in sheep, providing also an opportunity to characterize their different patterns on milk production aptitudeSIThis work is included in the framework of the project AGL2012-34437 funded by the Spanish Ministry of Economy and Competitiveness (MINECO). B.G.G. is funded through the Spanish ‘Ramón y Cajal’ Program (RYC-2012-10230) from the MINECONature ResearchProducción AnimalFacultad de Veterinaria2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://www.nature.com/articles/sdata201651https://hdl.handle.net/10612/24342reponame:BULERIA. Repositorio Institucional de la Universidad de Leóninstname:Universidad de LeónInglésinfo:eu-repo/grantAgreement/MINECO/Programa Nacional de Contratación e Incorporación/RYC-2012-10230http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:buleria.unileon.es:10612/243422026-06-24T12:43:27Z
dc.title.none.fl_str_mv Comprehensive RNA-Seq profiling to evaluate lactating sheep mammary gland transcriptome
title Comprehensive RNA-Seq profiling to evaluate lactating sheep mammary gland transcriptome
spellingShingle Comprehensive RNA-Seq profiling to evaluate lactating sheep mammary gland transcriptome
Suárez Vega, Aroa
Genética
Producción animal
Veterinaria
Time series design
Strain comparison design
Transcription profiling design
Transcription profiling assay
RNA sequencing
Selectively maintained organism
Time measurement datum
Ovis aries
Milk
3109 Ciencias Veterinarias
3104 Producción Animal
title_short Comprehensive RNA-Seq profiling to evaluate lactating sheep mammary gland transcriptome
title_full Comprehensive RNA-Seq profiling to evaluate lactating sheep mammary gland transcriptome
title_fullStr Comprehensive RNA-Seq profiling to evaluate lactating sheep mammary gland transcriptome
title_full_unstemmed Comprehensive RNA-Seq profiling to evaluate lactating sheep mammary gland transcriptome
title_sort Comprehensive RNA-Seq profiling to evaluate lactating sheep mammary gland transcriptome
dc.creator.none.fl_str_mv Suárez Vega, Aroa
Gutiérrez Gil, Beatriz
Klopp, Christophe
Tosser-Klopp, Gwenola
Arranz Santos, Juan José
author Suárez Vega, Aroa
author_facet Suárez Vega, Aroa
Gutiérrez Gil, Beatriz
Klopp, Christophe
Tosser-Klopp, Gwenola
Arranz Santos, Juan José
author_role author
author2 Gutiérrez Gil, Beatriz
Klopp, Christophe
Tosser-Klopp, Gwenola
Arranz Santos, Juan José
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Producción Animal
Facultad de Veterinaria
dc.subject.none.fl_str_mv Genética
Producción animal
Veterinaria
Time series design
Strain comparison design
Transcription profiling design
Transcription profiling assay
RNA sequencing
Selectively maintained organism
Time measurement datum
Ovis aries
Milk
3109 Ciencias Veterinarias
3104 Producción Animal
topic Genética
Producción animal
Veterinaria
Time series design
Strain comparison design
Transcription profiling design
Transcription profiling assay
RNA sequencing
Selectively maintained organism
Time measurement datum
Ovis aries
Milk
3109 Ciencias Veterinarias
3104 Producción Animal
description [EN] RNA-Seq enables the generation of extensive transcriptome information providing the capability to characterize transcripts (including alternative isoforms and polymorphism), to quantify expression and to identify differential regulation in a single experiment. Our aim in this study was to take advantage of using RNA-Seq high-throughput technology to provide a comprehensive transcriptome profiling of the sheep lactating mammary gland. Eight ewes of two dairy sheep breeds with differences in milk production traits were used in this experiment (four Churra and four Assaf ewes). Milk samples from these animals were collected on days 10, 50, 120 and 150 after lambing to cover the various physiological stages of the mammary gland across the complete lactation. RNA samples were extracted from milk somatic cells. The RNA-Seq dataset was generated using an Illumina HiSeq 2000 sequencer. The information reported here will be useful to understand the biology of lactation in sheep, providing also an opportunity to characterize their different patterns on milk production aptitude
publishDate 2016
dc.date.none.fl_str_mv 2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://www.nature.com/articles/sdata201651
https://hdl.handle.net/10612/24342
url https://www.nature.com/articles/sdata201651
https://hdl.handle.net/10612/24342
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/MINECO/Programa Nacional de Contratación e Incorporación/RYC-2012-10230
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Nature Research
publisher.none.fl_str_mv Nature Research
dc.source.none.fl_str_mv reponame:BULERIA. Repositorio Institucional de la Universidad de León
instname:Universidad de León
instname_str Universidad de León
reponame_str BULERIA. Repositorio Institucional de la Universidad de León
collection BULERIA. Repositorio Institucional de la Universidad de León
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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