Development and angiogenic potential of cell-derived microtissues using microcarrier-template

Tissue engineering and regenerative medicine approaches use biomaterials in combination with cells to regenerate lost functions of tissues and organs to prevent organ transplantation. However, most of the current strategies fail in mimicking the tissue’s extracellular matrix properties. In order to...

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Autores: Rubí Sans, Gerard|||0000-0003-2782-8716, Cano Garcia, Irene, Pérez Amodio, Soledad Graciela|||0000-0001-6825-0194, Blanco, Bárbara, Mateos Timoneda, Miguel Ángel|||0000-0001-7657-1414, Engel López, Elisabeth|||0000-0003-4855-8874
Tipo de recurso: artículo
Fecha de publicación:2021
País:España
Institución:Universitat Politècnica de Catalunya (UPC)
Repositorio:UPCommons. Portal del coneixement obert de la UPC
Idioma:inglés
OAI Identifier:oai:upcommons.upc.edu:2117/351411
Acceso en línea:https://hdl.handle.net/2117/351411
https://dx.doi.org/10.3390/biomedicines9030232
Access Level:acceso abierto
Palabra clave:Tissue engineering
Regenerative medicine
Biomedical materials
Poly-lactic acid microcarriers
Cultispher® S
Rat bone marrow mesenchymal stem cells
Microtissue
Cell-derived matrix
Angiogenesis
Enginyeria de teixits
Medicina regenerativa
Materials biomèdics
Àrees temàtiques de la UPC::Enginyeria dels materials
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spelling Development and angiogenic potential of cell-derived microtissues using microcarrier-templateRubí Sans, Gerard|||0000-0003-2782-8716Cano Garcia, IrenePérez Amodio, Soledad Graciela|||0000-0001-6825-0194Blanco, BárbaraMateos Timoneda, Miguel Ángel|||0000-0001-7657-1414Engel López, Elisabeth|||0000-0003-4855-8874Tissue engineeringRegenerative medicineBiomedical materialsPoly-lactic acid microcarriersCultispher® SRat bone marrow mesenchymal stem cellsMicrotissueCell-derived matrixAngiogenesisEnginyeria de teixitsMedicina regenerativaMaterials biomèdicsÀrees temàtiques de la UPC::Enginyeria dels materialsTissue engineering and regenerative medicine approaches use biomaterials in combination with cells to regenerate lost functions of tissues and organs to prevent organ transplantation. However, most of the current strategies fail in mimicking the tissue’s extracellular matrix properties. In order to mimic native tissue conditions, we developed cell-derived matrix (CDM) microtissues (MT). Our methodology uses poly-lactic acid (PLA) and Cultispher® S microcarriers’ (MCs’) as scaffold templates, which are seeded with rat bone marrow mesenchymal stem cells (rBM-MSCs). The scaffold template allows cells to generate an extracellular matrix, which is then extracted for downstream use. The newly formed CDM provides cells with a complex physical (MT architecture) and biochemical (deposited ECM proteins) environment, also showing spontaneous angiogenic potential. Our results suggest that MTs generated from the combination of these two MCs (mixed MTs) are excellent candidates for tissue vascularization. Overall, this study provides a methodology for in-house fabrication of microtissues with angiogenic potential for downstream use in various tissue regenerative strategies.Peer Reviewed20212021-02-2520212021-09-16journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/2117/351411https://dx.doi.org/10.3390/biomedicines9030232reponame:UPCommons. Portal del coneixement obert de la UPCinstname:Universitat Politècnica de Catalunya (UPC)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivates 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:upcommons.upc.edu:2117/3514112026-05-27T15:37:01Z
dc.title.none.fl_str_mv Development and angiogenic potential of cell-derived microtissues using microcarrier-template
title Development and angiogenic potential of cell-derived microtissues using microcarrier-template
spellingShingle Development and angiogenic potential of cell-derived microtissues using microcarrier-template
Rubí Sans, Gerard|||0000-0003-2782-8716
Tissue engineering
Regenerative medicine
Biomedical materials
Poly-lactic acid microcarriers
Cultispher® S
Rat bone marrow mesenchymal stem cells
Microtissue
Cell-derived matrix
Angiogenesis
Enginyeria de teixits
Medicina regenerativa
Materials biomèdics
Àrees temàtiques de la UPC::Enginyeria dels materials
title_short Development and angiogenic potential of cell-derived microtissues using microcarrier-template
title_full Development and angiogenic potential of cell-derived microtissues using microcarrier-template
title_fullStr Development and angiogenic potential of cell-derived microtissues using microcarrier-template
title_full_unstemmed Development and angiogenic potential of cell-derived microtissues using microcarrier-template
title_sort Development and angiogenic potential of cell-derived microtissues using microcarrier-template
dc.creator.none.fl_str_mv Rubí Sans, Gerard|||0000-0003-2782-8716
Cano Garcia, Irene
Pérez Amodio, Soledad Graciela|||0000-0001-6825-0194
Blanco, Bárbara
Mateos Timoneda, Miguel Ángel|||0000-0001-7657-1414
Engel López, Elisabeth|||0000-0003-4855-8874
author Rubí Sans, Gerard|||0000-0003-2782-8716
author_facet Rubí Sans, Gerard|||0000-0003-2782-8716
Cano Garcia, Irene
Pérez Amodio, Soledad Graciela|||0000-0001-6825-0194
Blanco, Bárbara
Mateos Timoneda, Miguel Ángel|||0000-0001-7657-1414
Engel López, Elisabeth|||0000-0003-4855-8874
author_role author
author2 Cano Garcia, Irene
Pérez Amodio, Soledad Graciela|||0000-0001-6825-0194
Blanco, Bárbara
Mateos Timoneda, Miguel Ángel|||0000-0001-7657-1414
Engel López, Elisabeth|||0000-0003-4855-8874
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Tissue engineering
Regenerative medicine
Biomedical materials
Poly-lactic acid microcarriers
Cultispher® S
Rat bone marrow mesenchymal stem cells
Microtissue
Cell-derived matrix
Angiogenesis
Enginyeria de teixits
Medicina regenerativa
Materials biomèdics
Àrees temàtiques de la UPC::Enginyeria dels materials
topic Tissue engineering
Regenerative medicine
Biomedical materials
Poly-lactic acid microcarriers
Cultispher® S
Rat bone marrow mesenchymal stem cells
Microtissue
Cell-derived matrix
Angiogenesis
Enginyeria de teixits
Medicina regenerativa
Materials biomèdics
Àrees temàtiques de la UPC::Enginyeria dels materials
description Tissue engineering and regenerative medicine approaches use biomaterials in combination with cells to regenerate lost functions of tissues and organs to prevent organ transplantation. However, most of the current strategies fail in mimicking the tissue’s extracellular matrix properties. In order to mimic native tissue conditions, we developed cell-derived matrix (CDM) microtissues (MT). Our methodology uses poly-lactic acid (PLA) and Cultispher® S microcarriers’ (MCs’) as scaffold templates, which are seeded with rat bone marrow mesenchymal stem cells (rBM-MSCs). The scaffold template allows cells to generate an extracellular matrix, which is then extracted for downstream use. The newly formed CDM provides cells with a complex physical (MT architecture) and biochemical (deposited ECM proteins) environment, also showing spontaneous angiogenic potential. Our results suggest that MTs generated from the combination of these two MCs (mixed MTs) are excellent candidates for tissue vascularization. Overall, this study provides a methodology for in-house fabrication of microtissues with angiogenic potential for downstream use in various tissue regenerative strategies.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021-02-25
2021
2021-09-16
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/2117/351411
https://dx.doi.org/10.3390/biomedicines9030232
url https://hdl.handle.net/2117/351411
https://dx.doi.org/10.3390/biomedicines9030232
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivates 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivates 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:UPCommons. Portal del coneixement obert de la UPC
instname:Universitat Politècnica de Catalunya (UPC)
instname_str Universitat Politècnica de Catalunya (UPC)
reponame_str UPCommons. Portal del coneixement obert de la UPC
collection UPCommons. Portal del coneixement obert de la UPC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.300719

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