Presence and Location of CatSper 1-4, Opioid (μ, δ and κ) and CD44 Receptors in SPERMATOZOA from AOUDAD, IBERIAN IBEX and Mouflon.

Despite the apparent progress in reproductive technologies in wild ruminant species, healthy live births have been limited. Acquiring a sound knowledge of the molecular basis of most functional aspects of spermatozoa will improve the effectiveness of reproductive techniques and optimise conservation...

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Autores: Vicente Carrillo, Alejandro, Álvarez Rodríguez, Manuel, Castaño, Cristina, Toledano Díaz, Adolfo, Martínez Nevado, Eva, Rodríguez Martínez, Heriberto, Santiago Moreno, Julián
Tipo de recurso: artículo
Fecha de publicación:2025
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/123011
Acceso en línea:https://hdl.handle.net/20.500.14352/123011
Access Level:acceso abierto
Palabra clave:636.082.4
Capacitation
Reproductive techniques
Semen
Wild ruminants
Producción animal
3104 Producción Animal
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spelling Presence and Location of CatSper 1-4, Opioid (μ, δ and κ) and CD44 Receptors in SPERMATOZOA from AOUDAD, IBERIAN IBEX and Mouflon.Vicente Carrillo, AlejandroÁlvarez Rodríguez, ManuelCastaño, CristinaToledano Díaz, AdolfoMartínez Nevado, EvaRodríguez Martínez, HeribertoSantiago Moreno, Julián636.082.4CapacitationReproductive techniquesSemenWild ruminantsProducción animal3104 Producción AnimalDespite the apparent progress in reproductive technologies in wild ruminant species, healthy live births have been limited. Acquiring a sound knowledge of the molecular basis of most functional aspects of spermatozoa will improve the effectiveness of reproductive techniques and optimise conservation programs for threatened species. CatSper channels, opioid receptors and CD44 are involved in sperm capacitation of humans and domestic animals, but their presence in wild ruminants is yet undisclosed. The aim of this study was to determine the presence and localisation of CatSper 1-4, μ, δ and κ-opioid receptors and CD44 in three wild ruminant species spermatozoa (aoudad [n = 5], Iberian ibex [n = 5], mouflon [n = 5]), which show different resistance to freezing-thawing processes. Western blotting (WB) and immunocytochemistry (ICC) performed with commercially available antibodies revealed that aoudad, Iberian ibex and mouflon are equipped with the aforementioned channels and receptors, sharing localisation with other domestic animals' spermatozoa but presenting species-particularities. WB revealed homogeneous results in CatSper 1, Catsper 2, Catsper 3 and CatSper 4 among the spermatozoa of the three species, unlike μ, δ and κ-opioid and CD44 receptors that showed substantial inter-species differences in the number of bands. ICC showed inter-species differences in the location of CatSper 1-4, μ, δ and κ-opioid and CD44 receptors. Data confirmed their presence and putative role on sperm function in wild ruminant species. Inter-species differences in the location of CatSper 1-4, μ, δ and κ-opioid and CD44 receptors might underlie the variable response to reproductive technologies in these species.WileyUniversidad Complutense de Madrid20252025-01-0120252025-01-01journal articlehttp://purl.org/coar/resource_type/c_6501AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/123011reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)InglésengAgencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023 PID2023-146957OB-I00 IMPACTO DE LOS RITMOS CIRCADIANOS EN LA FUNCIONALIDAD Y CRIORRESISTENCIA ESPERMATICA EN PEQUEÑOS RUMIANTES SILVESTRES Y DOMESTICOSopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/1230112026-06-02T12:44:21Z
dc.title.none.fl_str_mv Presence and Location of CatSper 1-4, Opioid (μ, δ and κ) and CD44 Receptors in SPERMATOZOA from AOUDAD, IBERIAN IBEX and Mouflon.
title Presence and Location of CatSper 1-4, Opioid (μ, δ and κ) and CD44 Receptors in SPERMATOZOA from AOUDAD, IBERIAN IBEX and Mouflon.
spellingShingle Presence and Location of CatSper 1-4, Opioid (μ, δ and κ) and CD44 Receptors in SPERMATOZOA from AOUDAD, IBERIAN IBEX and Mouflon.
Vicente Carrillo, Alejandro
636.082.4
Capacitation
Reproductive techniques
Semen
Wild ruminants
Producción animal
3104 Producción Animal
title_short Presence and Location of CatSper 1-4, Opioid (μ, δ and κ) and CD44 Receptors in SPERMATOZOA from AOUDAD, IBERIAN IBEX and Mouflon.
title_full Presence and Location of CatSper 1-4, Opioid (μ, δ and κ) and CD44 Receptors in SPERMATOZOA from AOUDAD, IBERIAN IBEX and Mouflon.
title_fullStr Presence and Location of CatSper 1-4, Opioid (μ, δ and κ) and CD44 Receptors in SPERMATOZOA from AOUDAD, IBERIAN IBEX and Mouflon.
title_full_unstemmed Presence and Location of CatSper 1-4, Opioid (μ, δ and κ) and CD44 Receptors in SPERMATOZOA from AOUDAD, IBERIAN IBEX and Mouflon.
title_sort Presence and Location of CatSper 1-4, Opioid (μ, δ and κ) and CD44 Receptors in SPERMATOZOA from AOUDAD, IBERIAN IBEX and Mouflon.
dc.creator.none.fl_str_mv Vicente Carrillo, Alejandro
Álvarez Rodríguez, Manuel
Castaño, Cristina
Toledano Díaz, Adolfo
Martínez Nevado, Eva
Rodríguez Martínez, Heriberto
Santiago Moreno, Julián
author Vicente Carrillo, Alejandro
author_facet Vicente Carrillo, Alejandro
Álvarez Rodríguez, Manuel
Castaño, Cristina
Toledano Díaz, Adolfo
Martínez Nevado, Eva
Rodríguez Martínez, Heriberto
Santiago Moreno, Julián
author_role author
author2 Álvarez Rodríguez, Manuel
Castaño, Cristina
Toledano Díaz, Adolfo
Martínez Nevado, Eva
Rodríguez Martínez, Heriberto
Santiago Moreno, Julián
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 636.082.4
Capacitation
Reproductive techniques
Semen
Wild ruminants
Producción animal
3104 Producción Animal
topic 636.082.4
Capacitation
Reproductive techniques
Semen
Wild ruminants
Producción animal
3104 Producción Animal
description Despite the apparent progress in reproductive technologies in wild ruminant species, healthy live births have been limited. Acquiring a sound knowledge of the molecular basis of most functional aspects of spermatozoa will improve the effectiveness of reproductive techniques and optimise conservation programs for threatened species. CatSper channels, opioid receptors and CD44 are involved in sperm capacitation of humans and domestic animals, but their presence in wild ruminants is yet undisclosed. The aim of this study was to determine the presence and localisation of CatSper 1-4, μ, δ and κ-opioid receptors and CD44 in three wild ruminant species spermatozoa (aoudad [n = 5], Iberian ibex [n = 5], mouflon [n = 5]), which show different resistance to freezing-thawing processes. Western blotting (WB) and immunocytochemistry (ICC) performed with commercially available antibodies revealed that aoudad, Iberian ibex and mouflon are equipped with the aforementioned channels and receptors, sharing localisation with other domestic animals' spermatozoa but presenting species-particularities. WB revealed homogeneous results in CatSper 1, Catsper 2, Catsper 3 and CatSper 4 among the spermatozoa of the three species, unlike μ, δ and κ-opioid and CD44 receptors that showed substantial inter-species differences in the number of bands. ICC showed inter-species differences in the location of CatSper 1-4, μ, δ and κ-opioid and CD44 receptors. Data confirmed their presence and putative role on sperm function in wild ruminant species. Inter-species differences in the location of CatSper 1-4, μ, δ and κ-opioid and CD44 receptors might underlie the variable response to reproductive technologies in these species.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025-01-01
2025
2025-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
AM
http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/123011
url https://hdl.handle.net/20.500.14352/123011
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023 PID2023-146957OB-I00 IMPACTO DE LOS RITMOS CIRCADIANOS EN LA FUNCIONALIDAD Y CRIORRESISTENCIA ESPERMATICA EN PEQUEÑOS RUMIANTES SILVESTRES Y DOMESTICOS
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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