miR-143 Interferes with ERK5 Signaling, and Abrogates Prostate Cancer Progression in Mice

Abstract Background: Micro RNAs are small, non-coding, single-stranded RNAs that negatively regulate gene expression at the post-transcriptional level. Since miR-143 was found to be down-regulated in prostate cancer cells, we wanted to analyze its expression in human prostate cancer, and test the ab...

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Autores: Clapé, Cyrielle, Fritz, Vanessa, Henriquet, Corinne, Apparailly, Florence, Fernández Ruiz, Pedro Luis, Iborra, François, Avancès, Christophe, Villalba, Martin, Culine, Stéphane, Fajas, Lluis
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2009
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/44087
Acceso en línea:https://hdl.handle.net/2445/44087
Access Level:acceso abierto
Palabra clave:Micro RNAs
Càncer de pròstata
Bioinformàtica
MicroRNAs
Prostate cancer
Bioinformatics
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spelling miR-143 Interferes with ERK5 Signaling, and Abrogates Prostate Cancer Progression in MiceClapé, CyrielleFritz, VanessaHenriquet, CorinneApparailly, FlorenceFernández Ruiz, Pedro LuisIborra, FrançoisAvancès, ChristopheVillalba, MartinCuline, StéphaneFajas, LluisMicro RNAsCàncer de pròstataBioinformàticaMicroRNAsProstate cancerBioinformaticsAbstract Background: Micro RNAs are small, non-coding, single-stranded RNAs that negatively regulate gene expression at the post-transcriptional level. Since miR-143 was found to be down-regulated in prostate cancer cells, we wanted to analyze its expression in human prostate cancer, and test the ability of miR-43 to arrest prostate cancer cell growth in vitro and in vivo. Results: Expression of miR-143 was analyzed in human prostate cancers by quantitative PCR, and by in situ hybridization. miR-143 was introduced in cancer cells in vivo by electroporation. Bioinformatics analysis and luciferase-based assays were used to determine miR-143 targets. We show in this study that miR-143 levels are inversely correlated with advanced stages of prostate cancer. Rescue of miR-143 expression in cancer cells results in the arrest of cell proliferation and the abrogation of tumor growth in mice. Furthermore, we show that the effects of miR-143 are mediated, at least in part by the inhibition of extracellular signal-regulated kinase-5 (ERK5) activity. We show here that ERK5 is a miR-143 target in prostate cancer. Conclusions: miR-143 is as a new target for prostate cancer treatment.Public Library of Science (PLoS)2009info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/44087Articles publicats en revistes (Fonaments Clínics)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: http://dx.doi.org/10.1371/journal.pone.0007542PLoS One, 2009, vol. 4, num. 10, p. e7542http://dx.doi.org/10.1371/journal.pone.0007542cc-by (c) Clapé, C. et al., 2009http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/440872026-05-27T06:46:51Z
dc.title.none.fl_str_mv miR-143 Interferes with ERK5 Signaling, and Abrogates Prostate Cancer Progression in Mice
title miR-143 Interferes with ERK5 Signaling, and Abrogates Prostate Cancer Progression in Mice
spellingShingle miR-143 Interferes with ERK5 Signaling, and Abrogates Prostate Cancer Progression in Mice
Clapé, Cyrielle
Micro RNAs
Càncer de pròstata
Bioinformàtica
MicroRNAs
Prostate cancer
Bioinformatics
title_short miR-143 Interferes with ERK5 Signaling, and Abrogates Prostate Cancer Progression in Mice
title_full miR-143 Interferes with ERK5 Signaling, and Abrogates Prostate Cancer Progression in Mice
title_fullStr miR-143 Interferes with ERK5 Signaling, and Abrogates Prostate Cancer Progression in Mice
title_full_unstemmed miR-143 Interferes with ERK5 Signaling, and Abrogates Prostate Cancer Progression in Mice
title_sort miR-143 Interferes with ERK5 Signaling, and Abrogates Prostate Cancer Progression in Mice
dc.creator.none.fl_str_mv Clapé, Cyrielle
Fritz, Vanessa
Henriquet, Corinne
Apparailly, Florence
Fernández Ruiz, Pedro Luis
Iborra, François
Avancès, Christophe
Villalba, Martin
Culine, Stéphane
Fajas, Lluis
author Clapé, Cyrielle
author_facet Clapé, Cyrielle
Fritz, Vanessa
Henriquet, Corinne
Apparailly, Florence
Fernández Ruiz, Pedro Luis
Iborra, François
Avancès, Christophe
Villalba, Martin
Culine, Stéphane
Fajas, Lluis
author_role author
author2 Fritz, Vanessa
Henriquet, Corinne
Apparailly, Florence
Fernández Ruiz, Pedro Luis
Iborra, François
Avancès, Christophe
Villalba, Martin
Culine, Stéphane
Fajas, Lluis
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Micro RNAs
Càncer de pròstata
Bioinformàtica
MicroRNAs
Prostate cancer
Bioinformatics
topic Micro RNAs
Càncer de pròstata
Bioinformàtica
MicroRNAs
Prostate cancer
Bioinformatics
description Abstract Background: Micro RNAs are small, non-coding, single-stranded RNAs that negatively regulate gene expression at the post-transcriptional level. Since miR-143 was found to be down-regulated in prostate cancer cells, we wanted to analyze its expression in human prostate cancer, and test the ability of miR-43 to arrest prostate cancer cell growth in vitro and in vivo. Results: Expression of miR-143 was analyzed in human prostate cancers by quantitative PCR, and by in situ hybridization. miR-143 was introduced in cancer cells in vivo by electroporation. Bioinformatics analysis and luciferase-based assays were used to determine miR-143 targets. We show in this study that miR-143 levels are inversely correlated with advanced stages of prostate cancer. Rescue of miR-143 expression in cancer cells results in the arrest of cell proliferation and the abrogation of tumor growth in mice. Furthermore, we show that the effects of miR-143 are mediated, at least in part by the inhibition of extracellular signal-regulated kinase-5 (ERK5) activity. We show here that ERK5 is a miR-143 target in prostate cancer. Conclusions: miR-143 is as a new target for prostate cancer treatment.
publishDate 2009
dc.date.none.fl_str_mv 2009
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/44087
url https://hdl.handle.net/2445/44087
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: http://dx.doi.org/10.1371/journal.pone.0007542
PLoS One, 2009, vol. 4, num. 10, p. e7542
http://dx.doi.org/10.1371/journal.pone.0007542
dc.rights.none.fl_str_mv cc-by (c) Clapé, C. et al., 2009
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Clapé, C. et al., 2009
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Public Library of Science (PLoS)
publisher.none.fl_str_mv Public Library of Science (PLoS)
dc.source.none.fl_str_mv Articles publicats en revistes (Fonaments Clínics)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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