A Novel Quantum Dot-Based pH Probe for Long-Term Fluorescence Lifetime Imaging Microscopy Experiments in Living Cells
The use of two nanoparticles for quantitative pH measurements in live cells by means of fluorescence lifetime imaging microscopy (FLIM) is investigated here. These results show the promising applications of this nanoprobe to monitor the intracellular pH and cell state employing the FLIM technique.
| Autores: | , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2022 |
| País: | España |
| Institución: | Universidad de Castilla-La Mancha |
| Repositorio: | RUIdeRA. Repositorio Institucional de la UCLM |
| OAI Identifier: | oai:ruidera.uclm.es:10578/36854 |
| Acceso en línea: | https://hdl.handle.net/10578/36854 |
| Access Level: | acceso abierto |
| Palabra clave: | Fluorescence lifetime probe Intracellular pH quantification Histidine-based nanoparticle Quantum dot |
| Sumario: | The use of two nanoparticles for quantitative pH measurements in live cells by means of fluorescence lifetime imaging microscopy (FLIM) is investigated here. These results show the promising applications of this nanoprobe to monitor the intracellular pH and cell state employing the FLIM technique. |
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