Genetic basis for denitrification in Ensifer meliloti

[Background] Denitrification is defined as the dissimilatory reduction of nitrate or nitrite to nitric oxide (NO), nitrous oxide (N2O), or dinitrogen gas (N2). N2O is a powerful atmospheric greenhouse gas and cause of ozone layer depletion. Legume crops might contribute to N2O production by providin...

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Autores: Torres Porras, María Jesús, Rubia, María I., Coba de la Peña, Teodoro, Pueyo, José Javier, Bedmar, Eulogio J., Delgado Igeño, María Jesús
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2014
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/125901
Acceso en línea:http://hdl.handle.net/10261/125901
Access Level:acceso abierto
Palabra clave:Cu-containing nitrite reductase
Nitrate respiration
Nitric oxide reductase
Nitrous oxide reductase
Periplasmic nitrate reductase
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spelling Genetic basis for denitrification in Ensifer melilotiTorres Porras, María JesúsRubia, María I.Coba de la Peña, TeodoroPueyo, José JavierBedmar, Eulogio J.Delgado Igeño, María JesúsCu-containing nitrite reductaseNitrate respirationNitric oxide reductaseNitrous oxide reductasePeriplasmic nitrate reductase[Background] Denitrification is defined as the dissimilatory reduction of nitrate or nitrite to nitric oxide (NO), nitrous oxide (N2O), or dinitrogen gas (N2). N2O is a powerful atmospheric greenhouse gas and cause of ozone layer depletion. Legume crops might contribute to N2O production by providing nitrogen-rich residues for decomposition or by associating with rhizobia that are able to denitrify under free-living and symbiotic conditions. However, there are limited direct empirical data concerning N2O production by endosymbiotic bacteria associated with legume crops. Analysis of the Ensifer meliloti 1021 genome sequence revealed the presence of the napEFDABC, nirK, norECBQD and nosRZDFYLX denitrification genes. It was recently reported that this bacterium is able to grow using nitrate respiration when cells are incubated with an initial O2 concentration of 2%; however, these cells were unable to use nitrate respiration when initially incubated anoxically. The involvement of the nap, nirK, nor and nos genes in E. meliloti denitrification has not been reported.[Results] E. meliloti nap, nirK and norC mutant strains exhibited defects in their ability to grow using nitrate as a respiratory substrate. However, E meliloti nosZ was not essential for growth under these conditions. The E. meliloti napA, nirK, norC and nosZ genes encode corresponding nitrate, nitrite, nitric oxide and nitrous oxide reductases, respectively. The NorC component of the E. meliloti nitric oxide reductase has been identified as a c-type cytochrome that is 16 kDa in size. Herein, we also show that maximal expression of the E. meliloti napA, nirK, norC and nosZ genes occurred when cells were initially incubated anoxically with nitrate.[Conclusion] The E. meliloti napA, nirK, norC and nosZ genes are involved in nitrate respiration and in the expression of denitrification enzymes in this bacterium. Our findings expand the short list of rhizobia for which denitrification gene function has been demonstrated. The inability of E. meliloti to grow when cells are initially subjected to anoxic conditions is not attributable to defects in the expression of the napA, nirK, norC and nosZ denitrification genes.This work was supported by a Fondo Europeo de Desarrollo Regional (FEDER)-co-financed grant (AGL2010-18607) and grant AGL2009-10371 from the Ministerio de Economía y Competitividad (Spain). Grant S2009/AMB-1511 from the Comunidad de Madrid and support from the Junta de Andalucía to Group BIO-275 are also acknowledged. We thank G. Tortosa for technical support and A. Becker for providing the E. meliloti mutants. MJT was supported by a fellowship from the Consejo Superior de Investigaciones Cientificas I3P Programme.BioMed CentralEuropean CommissionMinisterio de Economía y Competitividad (España)Comunidad de MadridJunta de AndalucíaConsejo Superior de Investigaciones Científicas (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2015201520142015info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/125901reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#S2009/AMB-1511/MICROAMBIENTE-CMhttp://dx.doi.org/10.1186/1471-2180-14-142Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1259012026-05-22T06:33:51Z
dc.title.none.fl_str_mv Genetic basis for denitrification in Ensifer meliloti
title Genetic basis for denitrification in Ensifer meliloti
spellingShingle Genetic basis for denitrification in Ensifer meliloti
Torres Porras, María Jesús
Cu-containing nitrite reductase
Nitrate respiration
Nitric oxide reductase
Nitrous oxide reductase
Periplasmic nitrate reductase
title_short Genetic basis for denitrification in Ensifer meliloti
title_full Genetic basis for denitrification in Ensifer meliloti
title_fullStr Genetic basis for denitrification in Ensifer meliloti
title_full_unstemmed Genetic basis for denitrification in Ensifer meliloti
title_sort Genetic basis for denitrification in Ensifer meliloti
dc.creator.none.fl_str_mv Torres Porras, María Jesús
Rubia, María I.
Coba de la Peña, Teodoro
Pueyo, José Javier
Bedmar, Eulogio J.
Delgado Igeño, María Jesús
author Torres Porras, María Jesús
author_facet Torres Porras, María Jesús
Rubia, María I.
Coba de la Peña, Teodoro
Pueyo, José Javier
Bedmar, Eulogio J.
Delgado Igeño, María Jesús
author_role author
author2 Rubia, María I.
Coba de la Peña, Teodoro
Pueyo, José Javier
Bedmar, Eulogio J.
Delgado Igeño, María Jesús
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv European Commission
Ministerio de Economía y Competitividad (España)
Comunidad de Madrid
Junta de Andalucía
Consejo Superior de Investigaciones Científicas (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Cu-containing nitrite reductase
Nitrate respiration
Nitric oxide reductase
Nitrous oxide reductase
Periplasmic nitrate reductase
topic Cu-containing nitrite reductase
Nitrate respiration
Nitric oxide reductase
Nitrous oxide reductase
Periplasmic nitrate reductase
description [Background] Denitrification is defined as the dissimilatory reduction of nitrate or nitrite to nitric oxide (NO), nitrous oxide (N2O), or dinitrogen gas (N2). N2O is a powerful atmospheric greenhouse gas and cause of ozone layer depletion. Legume crops might contribute to N2O production by providing nitrogen-rich residues for decomposition or by associating with rhizobia that are able to denitrify under free-living and symbiotic conditions. However, there are limited direct empirical data concerning N2O production by endosymbiotic bacteria associated with legume crops. Analysis of the Ensifer meliloti 1021 genome sequence revealed the presence of the napEFDABC, nirK, norECBQD and nosRZDFYLX denitrification genes. It was recently reported that this bacterium is able to grow using nitrate respiration when cells are incubated with an initial O2 concentration of 2%; however, these cells were unable to use nitrate respiration when initially incubated anoxically. The involvement of the nap, nirK, nor and nos genes in E. meliloti denitrification has not been reported.
publishDate 2014
dc.date.none.fl_str_mv 2014
2015
2015
2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/125901
url http://hdl.handle.net/10261/125901
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
S2009/AMB-1511/MICROAMBIENTE-CM
http://dx.doi.org/10.1186/1471-2180-14-142
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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