Supplementary Information for Engineering pan-HIV-1 neutralization potency through multispecific antibody avidity
13 pages. -- This PDF file includes: Figures S1 to S7 and Tables S1 to S2. -- Fig. S1. Characterization of scFab-apoferritin fusions. -- Fig. S2. Multabody affinity-purification scheme. Protein A and Protein L sequential affinity purification. -- Fig. S3. Generation of a Multabody that cross-targets...
| Autores: | , , , , , , , , , , , , |
|---|---|
| Formato: | conjunto de datos |
| Fecha de publicación: | 2022 |
| País: | España |
| Recursos: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/331427 |
| Acesso em linha: | http://hdl.handle.net/10261/331427 |
| Access Level: | acceso abierto |
| Resumo: | 13 pages. -- This PDF file includes: Figures S1 to S7 and Tables S1 to S2. -- Fig. S1. Characterization of scFab-apoferritin fusions. -- Fig. S2. Multabody affinity-purification scheme. Protein A and Protein L sequential affinity purification. -- Fig. S3. Generation of a Multabody that cross-targets the HIV-1 Env and the CD4 receptor. -- Fig. S4. Biophysical characterization of HIV-1 Multabodies. Comparison of the Tm and Tagg temperatures of T-01/T-02 MB, 12-mer ferritin fusions, parental IgGs and the N6/PGDM1400x10E8v4 trispecific antibody. -- Fig. S5. Binding characteristics of IgGs binding to four different antigens. -- Fig. S6. Multabody v2 features. -- Fig. S7. PsV neutralization and inhibition of primary PBMC infection by Multabodies. -- Table S1. IC50 of individual and IgG mixtures, PGDM1400/N6x10E8v4 trispecific and HIV-1 Multabodies against a 14-PsV panel and 25-PsV panel (additional 11 HIV-1 strains highly resistant to PGDM1400). -- Table S2. Potency of parental and IgG mixtures and T-01 Multabody versions against a 118-PsV panel. |
|---|