Cutibacterium acnes strain improvement and establishment of skin microbiome sequencing technologies
Certain skin disorders are associated with skin microbiome dysbiosis, highlighting the importance of the microbiome in the maintenance of the skin's healthy state. Modulating the microbiome to reestablish its healthy state is a novel strategy to treat such skin diseases. One strategy to modulat...
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| Tipo de recurso: | tesis doctoral |
| Estado: | Versión publicada |
| Fecha de publicación: | 2022 |
| País: | España |
| Institución: | CBUC, CESCA |
| Repositorio: | TDR. Tesis Doctorales en Red |
| OAI Identifier: | oai:www.tdx.cat:10803/687391 |
| Acceso en línea: | http://hdl.handle.net/10803/687391 |
| Access Level: | acceso abierto |
| Palabra clave: | Skin Microbiome Sequencing Probiotics Cutibacterium Pell Microbioma Sequenciacio 616.5 |
| Sumario: | Certain skin disorders are associated with skin microbiome dysbiosis, highlighting the importance of the microbiome in the maintenance of the skin's healthy state. Modulating the microbiome to reestablish its healthy state is a novel strategy to treat such skin diseases. One strategy to modulate the skin microbiome and obtain dermatological benefits is through the topical application of probiotics. Identifying and selecting the most suitable bacterial strains to treat specific skin diseases is key to success. In this research a Cutibacterium acnes strain phenotype was improved using non-GMO techniques, making its usage as a probiotic for more skin diseases possible. Furthermore, taxonomic identification of skin microbiome samples using Nanopore sequencing was benchmarked and improved, contributing to advancements in both diagnosis and novel treatments. Finally, a probiotic cocktail was clinically evaluated on patients suffering from acne vulgaris, showing dermatological benefits as well as skin microbiome modulation effects. In summary, this research contributed to the advancement in treating skin diseases by generating new probiotics, benchmarking and improving bacterial taxonomic identification using Nanopore sequencing and proving the benefits of a bacterial cocktail in treating acne vulgaris. |
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