Revising Protein Corona Characterization and Combining ITC and Nano-DSC to Understand the Interaction of Proteins With Porous Nanoparticles

The exposure of nanoparticles (NPs) to biological fluids leads to the formation of a protein coating that is known as protein corona (PC). Since PC formation is influenced by the physicochemical properties of the nanoparticles, the understanding of the interplay of the factors that participate in th...

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Detalhes bibliográficos
Autores: Balmori, Alba, Sandu, Romica, Gheorghe, Daniela, Precupas, Aurica, Tanasescu, Speranta, Sánchez-García, David, Borrós, Salvador
Tipo de documento: artigo
Data de publicação:2021
País:España
Recursos:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositório:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:20.500.14342/4475
Acesso em linha:http://hdl.handle.net/20.500.14342/4475
https://doi.org/10.3389/fbioe.2021.650281
Access Level:Acceso aberto
Palavra-chave:Protein corona
Nano DSC
ITC characterization
Mesoporous silica nanoparticles (MSN)
577
612
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spelling Revising Protein Corona Characterization and Combining ITC and Nano-DSC to Understand the Interaction of Proteins With Porous NanoparticlesBalmori, AlbaSandu, RomicaGheorghe, DanielaPrecupas, AuricaTanasescu, SperantaSánchez-García, DavidBorrós, SalvadorProtein coronaNano DSCITC characterizationMesoporous silica nanoparticles (MSN)577612The exposure of nanoparticles (NPs) to biological fluids leads to the formation of a protein coating that is known as protein corona (PC). Since PC formation is influenced by the physicochemical properties of the nanoparticles, the understanding of the interplay of the factors that participate in this process is crucial for the development of nanomaterials as cell-targeted delivery vehicles. In general, it is accepted that the PC formation is a complex and dynamic process, which depends on the composition of the medium and the properties of the NP mainly size, shape, and superficial charge. Interestingly, although the interaction between the protein and the NP is essentially a superficial phenomenon, the influence of the roughness of the nanoparticle surface has been scarcely studied. In this work, the influence of superficial roughness and porosity has been studied with the aid of nanodifferential scanning calorimetry (nano-DSC) and isothermal titration calorimetry (ITC) using mesoporous silica nanoparticles (MSNs) as an NP model. The interaction process of the proteins with the NP surface was analyzed by ITC measurements, while the stability and denaturation of the proteins was monitored by nano-DSC. Thanks to the complementarity of these two techniques, a more complete insight into the PC formation on the pores has been accomplished.info:eu-repo/semantics/publishedVersionFrontiers MediaUniversitat Ramon Llull. IQS2021info:eu-repo/semantics/article9 p.http://hdl.handle.net/20.500.14342/4475https://doi.org/10.3389/fbioe.2021.650281reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésFrontiers in Bioengineering and Biotechnologyinfo:eu-repo/grantAgreement/SUR del DEC/SGR/SGR 2017 1559info:eu-repo/grantAgreement/MCIU/PN I+D/RTI2018-094734-B-C22© L'autor/aAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:20.500.14342/44752026-05-29T05:05:01Z
dc.title.none.fl_str_mv Revising Protein Corona Characterization and Combining ITC and Nano-DSC to Understand the Interaction of Proteins With Porous Nanoparticles
title Revising Protein Corona Characterization and Combining ITC and Nano-DSC to Understand the Interaction of Proteins With Porous Nanoparticles
spellingShingle Revising Protein Corona Characterization and Combining ITC and Nano-DSC to Understand the Interaction of Proteins With Porous Nanoparticles
Balmori, Alba
Protein corona
Nano DSC
ITC characterization
Mesoporous silica nanoparticles (MSN)
577
612
title_short Revising Protein Corona Characterization and Combining ITC and Nano-DSC to Understand the Interaction of Proteins With Porous Nanoparticles
title_full Revising Protein Corona Characterization and Combining ITC and Nano-DSC to Understand the Interaction of Proteins With Porous Nanoparticles
title_fullStr Revising Protein Corona Characterization and Combining ITC and Nano-DSC to Understand the Interaction of Proteins With Porous Nanoparticles
title_full_unstemmed Revising Protein Corona Characterization and Combining ITC and Nano-DSC to Understand the Interaction of Proteins With Porous Nanoparticles
title_sort Revising Protein Corona Characterization and Combining ITC and Nano-DSC to Understand the Interaction of Proteins With Porous Nanoparticles
dc.creator.none.fl_str_mv Balmori, Alba
Sandu, Romica
Gheorghe, Daniela
Precupas, Aurica
Tanasescu, Speranta
Sánchez-García, David
Borrós, Salvador
author Balmori, Alba
author_facet Balmori, Alba
Sandu, Romica
Gheorghe, Daniela
Precupas, Aurica
Tanasescu, Speranta
Sánchez-García, David
Borrós, Salvador
author_role author
author2 Sandu, Romica
Gheorghe, Daniela
Precupas, Aurica
Tanasescu, Speranta
Sánchez-García, David
Borrós, Salvador
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universitat Ramon Llull. IQS
dc.subject.none.fl_str_mv Protein corona
Nano DSC
ITC characterization
Mesoporous silica nanoparticles (MSN)
577
612
topic Protein corona
Nano DSC
ITC characterization
Mesoporous silica nanoparticles (MSN)
577
612
description The exposure of nanoparticles (NPs) to biological fluids leads to the formation of a protein coating that is known as protein corona (PC). Since PC formation is influenced by the physicochemical properties of the nanoparticles, the understanding of the interplay of the factors that participate in this process is crucial for the development of nanomaterials as cell-targeted delivery vehicles. In general, it is accepted that the PC formation is a complex and dynamic process, which depends on the composition of the medium and the properties of the NP mainly size, shape, and superficial charge. Interestingly, although the interaction between the protein and the NP is essentially a superficial phenomenon, the influence of the roughness of the nanoparticle surface has been scarcely studied. In this work, the influence of superficial roughness and porosity has been studied with the aid of nanodifferential scanning calorimetry (nano-DSC) and isothermal titration calorimetry (ITC) using mesoporous silica nanoparticles (MSNs) as an NP model. The interaction process of the proteins with the NP surface was analyzed by ITC measurements, while the stability and denaturation of the proteins was monitored by nano-DSC. Thanks to the complementarity of these two techniques, a more complete insight into the PC formation on the pores has been accomplished.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.14342/4475
https://doi.org/10.3389/fbioe.2021.650281
url http://hdl.handle.net/20.500.14342/4475
https://doi.org/10.3389/fbioe.2021.650281
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Frontiers in Bioengineering and Biotechnology
info:eu-repo/grantAgreement/SUR del DEC/SGR/SGR 2017 1559
info:eu-repo/grantAgreement/MCIU/PN I+D/RTI2018-094734-B-C22
dc.rights.none.fl_str_mv © L'autor/a
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv © L'autor/a
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 9 p.
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.81155

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