Kainate receptor activation shapes short-term synaptic plasticity by controlling receptor lateral Mobility at glutamatergic synapses

Kainate receptors (KARs) mediate postsynaptic currents with a key impact on neuronal excitability. However, the molecular determinants controlling KAR postsynaptic localization and stabilization are poorly understood. Here, we exploit optogenetic and single-particle tracking approaches to study the...

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Detalhes bibliográficos
Autores: Polenghi, Alice, Nieus, Thierry, Guazzi, Stefania, Gorostiza Langa, Pablo Ignacio, Petrini, Enrica Maria, Barberis, Andrea
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2020
País:España
Recursos:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositório:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/177572
Acesso em linha:https://hdl.handle.net/2445/177572
Access Level:Acceso aberto
Palavra-chave:Sinapsi
Receptors neurals
Neurotransmissió
Synapses
Neural receptor
Neural transmission
Descrição
Resumo:Kainate receptors (KARs) mediate postsynaptic currents with a key impact on neuronal excitability. However, the molecular determinants controlling KAR postsynaptic localization and stabilization are poorly understood. Here, we exploit optogenetic and single-particle tracking approaches to study the role of KAR conformational states induced by glutamate binding on KAR lateral mobility at synapses. We report that following glutamate binding, KARs are readily and reversibly trapped at glutamatergic synapses through increased interaction with the β-catenin/N-cadherin complex. We demonstrate that such activation-dependent synaptic immobilization of KARs is crucial for the modulation of short-term plasticity of glutamatergic synapses. Thus, the present study unveils the crosstalk between conformational states and lateral mobility of KARs, a mechanism regulating glutamatergic signaling, particularly in conditions of sustained synaptic activity.