Isolation and characterization of microsatellite loci for the endangered Midwife Betic toad Alytes dickhilleni (Discoglossidae)

We have developed eleven polymorphic micro- satellite loci for the amphibian Alytes dickhilleni using an enriched-library approach. We detected 98 alleles in 50 individuals genotyped (mean number of alleles per locus was 8.91) in two different populations in South East Spain. Expected heterozygositi...

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Detalhes bibliográficos
Autores: Albert, Eva M., Arroyo, Juan M., Godoy, José A.
Formato: artículo
Fecha de publicación:2011
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/58616
Acesso em linha:http://hdl.handle.net/10261/58616
Access Level:acceso abierto
Descrição
Resumo:We have developed eleven polymorphic micro- satellite loci for the amphibian Alytes dickhilleni using an enriched-library approach. We detected 98 alleles in 50 individuals genotyped (mean number of alleles per locus was 8.91) in two different populations in South East Spain. Expected heterozygosities ranged from 0.324 to 0.891 in one population (Arroyo Guadahornillos) and 0.424–0.909 in the other population (Cueva Paria). The levels of poly- morphism of the developed markers render them readily applicable for population genetic studies of diversity, structure, and migration.