Regulation of cortico-thalamic JNK1/2 and ERK1/2 MAPKs and apoptosis-related signaling pathways in PDYN gene-deficient mice following acute and chronic mild stress

This article belongs to the Special Issue Involvement of the MAPK Pathway in Cancer and Immunology.

Bibliographic Details
Authors: Yáñez-Gómez, Fernando, Ramos-Miguel, Alfredo, García-Sevilla, Jesús Andrés, Manzanares, Jorge, Femenía, Teresa
Format: article
Status:Published version
Publication Date:2023
Country:España
Institution:Consejo Superior de Investigaciones Científicas (CSIC)
Repository:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/309073
Online Access:http://hdl.handle.net/10261/309073
Access Level:Open access
Keyword:Prodynorphin
Phosphorylation
JNK
Apoptosis
ERK
Chronic mild stress
id ES_d70f4f35039ea4be5ab0fa800ca6bddd
oai_identifier_str oai:digital.csic.es:10261/309073
network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv Regulation of cortico-thalamic JNK1/2 and ERK1/2 MAPKs and apoptosis-related signaling pathways in PDYN gene-deficient mice following acute and chronic mild stress
title Regulation of cortico-thalamic JNK1/2 and ERK1/2 MAPKs and apoptosis-related signaling pathways in PDYN gene-deficient mice following acute and chronic mild stress
spellingShingle Regulation of cortico-thalamic JNK1/2 and ERK1/2 MAPKs and apoptosis-related signaling pathways in PDYN gene-deficient mice following acute and chronic mild stress
Yáñez-Gómez, Fernando
Prodynorphin
Phosphorylation
JNK
Apoptosis
ERK
Chronic mild stress
title_short Regulation of cortico-thalamic JNK1/2 and ERK1/2 MAPKs and apoptosis-related signaling pathways in PDYN gene-deficient mice following acute and chronic mild stress
title_full Regulation of cortico-thalamic JNK1/2 and ERK1/2 MAPKs and apoptosis-related signaling pathways in PDYN gene-deficient mice following acute and chronic mild stress
title_fullStr Regulation of cortico-thalamic JNK1/2 and ERK1/2 MAPKs and apoptosis-related signaling pathways in PDYN gene-deficient mice following acute and chronic mild stress
title_full_unstemmed Regulation of cortico-thalamic JNK1/2 and ERK1/2 MAPKs and apoptosis-related signaling pathways in PDYN gene-deficient mice following acute and chronic mild stress
title_sort Regulation of cortico-thalamic JNK1/2 and ERK1/2 MAPKs and apoptosis-related signaling pathways in PDYN gene-deficient mice following acute and chronic mild stress
dc.creator.none.fl_str_mv Yáñez-Gómez, Fernando
Ramos-Miguel, Alfredo
García-Sevilla, Jesús Andrés
Manzanares, Jorge
Femenía, Teresa
author Yáñez-Gómez, Fernando
author_facet Yáñez-Gómez, Fernando
Ramos-Miguel, Alfredo
García-Sevilla, Jesús Andrés
Manzanares, Jorge
Femenía, Teresa
author_role author
author2 Ramos-Miguel, Alfredo
García-Sevilla, Jesús Andrés
Manzanares, Jorge
Femenía, Teresa
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Salud Carlos III
European Commission
Plan Nacional sobre Drogas (España)
Ministerio de Sanidad (España)
Ministerio de Ciencia, Innovación y Universidades (España)
Agencia Estatal de Investigación (España)
Ministerio de Ciencia e Innovación (España)
Ministerio de Economía y Competitividad (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Prodynorphin
Phosphorylation
JNK
Apoptosis
ERK
Chronic mild stress
topic Prodynorphin
Phosphorylation
JNK
Apoptosis
ERK
Chronic mild stress
description This article belongs to the Special Issue Involvement of the MAPK Pathway in Cancer and Immunology.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/309073
url http://hdl.handle.net/10261/309073
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
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info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-094414-A-I00
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-109323RA-I00
info:eu-repo/grantAgreement/MICINN//SAF2008-01311
info:eu-repo/grantAgreement/MINECO//RYC-2016-19282
info:eu-repo/grantAgreement/AEI//RYC-2017-22666
International Journal of Molecular Sciences
The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.3390/ijms24032303
https://doi.org/10.3390/ijms24032303
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
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spelling Regulation of cortico-thalamic JNK1/2 and ERK1/2 MAPKs and apoptosis-related signaling pathways in PDYN gene-deficient mice following acute and chronic mild stressYáñez-Gómez, FernandoRamos-Miguel, AlfredoGarcía-Sevilla, Jesús AndrésManzanares, JorgeFemenía, TeresaProdynorphinPhosphorylationJNKApoptosisERKChronic mild stressThis article belongs to the Special Issue Involvement of the MAPK Pathway in Cancer and Immunology.The crosstalk between the opioidergic system and mitogen-activated protein kinases (MAPKs) has a critical role in mediating stress-induced behaviors related to the pathophysiology of anxiety. The present study evaluated the basal status and stress-induced alterations of cortico-thalamic MAPKs and other cell fate-related signaling pathways potentially underlying the anxiogenic endophenotype of PDYN gene-deficient mice. Compared to littermates, PDYN knockout (KO) mice had lower cortical and or thalamic amounts of the phospho-activated MAPKs c-Jun N-terminal kinase (JNK1/2) and extracellular signal-regulated kinase (ERK1/2). Similarly, PDYN-KO animals displayed reduced cortico-thalamic densities of total and phosphorylated (at Ser191) species of the cell fate regulator Fas-associated protein with death domain (FADD) without alterations in the Fas receptor. Exposure to acute restraint and chronic mild stress stimuli induced the robust stimulation of JNK1/2 and ERK1/2 MAPKs, FADD, and Akt-mTOR pathways, without apparent increases in apoptotic rates. Interestingly, PDYN deficiency prevented stress-induced JNK1/2 and FADD but not ERK1/2 or Akt-mTOR hyperactivations. These findings suggest that cortico-thalamic MAPK- and FADD-dependent neuroplasticity might be altered in PDYN-KO mice. In addition, the results also indicate that the PDYN gene (and hence dynorphin release) may be required to stimulate JNK1/2 and FADD (but not ERK1/2 or Akt/mTOR) pathways under environmental stress conditions.This joint research was funded by Red Temática de Investigación Cooperativa en Salud–Red de Trastornos Adictivos (RETICS–RTA, Instituto de Salud Carlos III [ISCIII], MCIU/AEI/FEDER), Grupos RD06/0001/0004 (J.M.) and RD06/0001/0003 (J.A.G.-S.). J.M. also received financial support from Proyectos de Investigación en Salud—ISCIII (grant RD. PI18/00576), Red de Investigación en Atención Primaria de Adicciones (grant RD21/0009/0008), and Delegación del Gobierno para el Plan Nacional Sobre Drogas (PNSD, grant 2019I012) from the Spanish Ministry of Health (MSC). This study was also supported by MCIU/AEI/FEDER (grants RTI2018-094414-A-I00 to A.R.-M., PID2019-109323RA-I00 to T.F., and SAF2008-01311 to J.A.G.-S.), and MSC/FEDER (FIS 05/0429 to J.M). A.R.-M. (grant RYC-2016-19282) and T.F. (grant RYC-2017-22666) are ‘Ramón y Cajal’ Researchers.Peer reviewedMultidisciplinary Digital Publishing InstituteInstituto de Salud Carlos IIIEuropean CommissionPlan Nacional sobre Drogas (España)Ministerio de Sanidad (España)Ministerio de Ciencia, Innovación y Universidades (España)Agencia Estatal de Investigación (España)Ministerio de Ciencia e Innovación (España)Ministerio de Economía y Competitividad (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/309073reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-094414-A-I00info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-109323RA-I00info:eu-repo/grantAgreement/MICINN//SAF2008-01311info:eu-repo/grantAgreement/MINECO//RYC-2016-19282info:eu-repo/grantAgreement/AEI//RYC-2017-22666International Journal of Molecular SciencesThe underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.3390/ijms24032303https://doi.org/10.3390/ijms24032303Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3090732026-05-22T06:33:51Z
score 15.811543

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