Stenotrophomonas maltophilia responds to exogenous AHL signals through the LuxR solo SmoR (Smlt1839)

Quorum Sensing (QS) mediated by Acyl Homoserine Lactone (AHL) molecules are probably the most widespread and studied among Gram-negative bacteria. Canonical AHL systems are composed by a synthase (LuxI family) and a regulator element (LuxR family), whose genes are usually adjacent in the genome. How...

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Autores: Martínez Alcalá, Paula, Huedo Moreno, Pol|||0000-0002-2933-0586, Martínez-Servat, Sònia, Planell Cerezo, Raquel|||0009-0003-9513-0245, Ferrer-Navarro, Mario|||0000-0001-9990-914X, Daura i Ribera, Xavier|||0000-0001-9235-6730, Yero, Daniel|||0000-0002-6234-4082, Gibert, Isidre|||0000-0003-1442-2258
Tipo de recurso: artículo
Fecha de publicación:2015
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:catalán
OAI Identifier:oai:ddd.uab.cat:154676
Acceso en línea:https://ddd.uab.cat/record/154676
https://dx.doi.org/urn:doi:10.3389/fcimb.2015.00041
Access Level:acceso abierto
Palabra clave:LuxR Orphan
AHL
Acyl-Homoserine lactone
Lactonase
Quorum sensing
Swarming
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spelling Stenotrophomonas maltophilia responds to exogenous AHL signals through the LuxR solo SmoR (Smlt1839)Martínez Alcalá, PaulaHuedo Moreno, Pol|||0000-0002-2933-0586Martínez-Servat, SòniaPlanell Cerezo, Raquel|||0009-0003-9513-0245Ferrer-Navarro, Mario|||0000-0001-9990-914XDaura i Ribera, Xavier|||0000-0001-9235-6730Yero, Daniel|||0000-0002-6234-4082Gibert, Isidre|||0000-0003-1442-2258LuxR OrphanAHLAcyl-Homoserine lactoneLactonaseQuorum sensingSwarmingQuorum Sensing (QS) mediated by Acyl Homoserine Lactone (AHL) molecules are probably the most widespread and studied among Gram-negative bacteria. Canonical AHL systems are composed by a synthase (LuxI family) and a regulator element (LuxR family), whose genes are usually adjacent in the genome. However, incomplete AHL-QS machinery lacking the synthase LuxI is frequently observed in Proteobacteria, and the regulator element is then referred as LuxR solo. It has been shown that certain LuxR solos participate in interspecific communication by detecting signals produced by different organisms. In the case of Stenotrophomonas maltophilia, a preliminary genome sequence analysis revealed numerous putative luxR genes, none of them associated to a luxI gene. From these, the hypothetical LuxR solo Smlt1839, here designated SmoR, presents a conserved AHL binding domain and a helix-turn-helix DNA binding motif. Its genomic organization-adjacent to hchA gene-indicate that SmoR belongs to the new family "LuxR regulator chaperone HchA-associated." AHL-binding assays revealed that SmoR binds to AHLs in-vitro, at least to oxo-C8-homoserine lactone, and it regulates operon transcription, likely by recognizing a conserved palindromic regulatory box in the hchA upstream region. Supplementation with concentrated supernatants from Pseudomonas aeruginosa, which contain significant amounts of AHLs, promoted swarming motility in S. maltophilia. Contrarily, no swarming stimulation was observed when the P. aeruginosa supernatant was treated with the lactonase AiiA from Bacillus subtilis, confirming that AHL contributes to enhance the swarming ability of S. maltophilia. Finally, mutation of smoR resulted in a swarming alteration and an apparent insensitivity to the exogenous AHLs provided by P. aeruginosa. In conclusion, our results demonstrate that S. maltophilia senses AHLs produced by neighboring bacteria through the LuxR solo SmoR, regulating population behaviors such as swarming motility. 22015-01-0120152015-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/154676https://dx.doi.org/urn:doi:10.3389/fcimb.2015.00041reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaCataláncatEuropean Commission https://doi.org/10.13039/501100000780 223101Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2014/SGR-1280open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/3.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:1546762026-06-06T12:50:31Z
dc.title.none.fl_str_mv Stenotrophomonas maltophilia responds to exogenous AHL signals through the LuxR solo SmoR (Smlt1839)
title Stenotrophomonas maltophilia responds to exogenous AHL signals through the LuxR solo SmoR (Smlt1839)
spellingShingle Stenotrophomonas maltophilia responds to exogenous AHL signals through the LuxR solo SmoR (Smlt1839)
Martínez Alcalá, Paula
LuxR Orphan
AHL
Acyl-Homoserine lactone
Lactonase
Quorum sensing
Swarming
title_short Stenotrophomonas maltophilia responds to exogenous AHL signals through the LuxR solo SmoR (Smlt1839)
title_full Stenotrophomonas maltophilia responds to exogenous AHL signals through the LuxR solo SmoR (Smlt1839)
title_fullStr Stenotrophomonas maltophilia responds to exogenous AHL signals through the LuxR solo SmoR (Smlt1839)
title_full_unstemmed Stenotrophomonas maltophilia responds to exogenous AHL signals through the LuxR solo SmoR (Smlt1839)
title_sort Stenotrophomonas maltophilia responds to exogenous AHL signals through the LuxR solo SmoR (Smlt1839)
dc.creator.none.fl_str_mv Martínez Alcalá, Paula
Huedo Moreno, Pol|||0000-0002-2933-0586
Martínez-Servat, Sònia
Planell Cerezo, Raquel|||0009-0003-9513-0245
Ferrer-Navarro, Mario|||0000-0001-9990-914X
Daura i Ribera, Xavier|||0000-0001-9235-6730
Yero, Daniel|||0000-0002-6234-4082
Gibert, Isidre|||0000-0003-1442-2258
author Martínez Alcalá, Paula
author_facet Martínez Alcalá, Paula
Huedo Moreno, Pol|||0000-0002-2933-0586
Martínez-Servat, Sònia
Planell Cerezo, Raquel|||0009-0003-9513-0245
Ferrer-Navarro, Mario|||0000-0001-9990-914X
Daura i Ribera, Xavier|||0000-0001-9235-6730
Yero, Daniel|||0000-0002-6234-4082
Gibert, Isidre|||0000-0003-1442-2258
author_role author
author2 Huedo Moreno, Pol|||0000-0002-2933-0586
Martínez-Servat, Sònia
Planell Cerezo, Raquel|||0009-0003-9513-0245
Ferrer-Navarro, Mario|||0000-0001-9990-914X
Daura i Ribera, Xavier|||0000-0001-9235-6730
Yero, Daniel|||0000-0002-6234-4082
Gibert, Isidre|||0000-0003-1442-2258
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv LuxR Orphan
AHL
Acyl-Homoserine lactone
Lactonase
Quorum sensing
Swarming
topic LuxR Orphan
AHL
Acyl-Homoserine lactone
Lactonase
Quorum sensing
Swarming
description Quorum Sensing (QS) mediated by Acyl Homoserine Lactone (AHL) molecules are probably the most widespread and studied among Gram-negative bacteria. Canonical AHL systems are composed by a synthase (LuxI family) and a regulator element (LuxR family), whose genes are usually adjacent in the genome. However, incomplete AHL-QS machinery lacking the synthase LuxI is frequently observed in Proteobacteria, and the regulator element is then referred as LuxR solo. It has been shown that certain LuxR solos participate in interspecific communication by detecting signals produced by different organisms. In the case of Stenotrophomonas maltophilia, a preliminary genome sequence analysis revealed numerous putative luxR genes, none of them associated to a luxI gene. From these, the hypothetical LuxR solo Smlt1839, here designated SmoR, presents a conserved AHL binding domain and a helix-turn-helix DNA binding motif. Its genomic organization-adjacent to hchA gene-indicate that SmoR belongs to the new family "LuxR regulator chaperone HchA-associated." AHL-binding assays revealed that SmoR binds to AHLs in-vitro, at least to oxo-C8-homoserine lactone, and it regulates operon transcription, likely by recognizing a conserved palindromic regulatory box in the hchA upstream region. Supplementation with concentrated supernatants from Pseudomonas aeruginosa, which contain significant amounts of AHLs, promoted swarming motility in S. maltophilia. Contrarily, no swarming stimulation was observed when the P. aeruginosa supernatant was treated with the lactonase AiiA from Bacillus subtilis, confirming that AHL contributes to enhance the swarming ability of S. maltophilia. Finally, mutation of smoR resulted in a swarming alteration and an apparent insensitivity to the exogenous AHLs provided by P. aeruginosa. In conclusion, our results demonstrate that S. maltophilia senses AHLs produced by neighboring bacteria through the LuxR solo SmoR, regulating population behaviors such as swarming motility.
publishDate 2015
dc.date.none.fl_str_mv 2
2015-01-01
2015
2015-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/154676
https://dx.doi.org/urn:doi:10.3389/fcimb.2015.00041
url https://ddd.uab.cat/record/154676
https://dx.doi.org/urn:doi:10.3389/fcimb.2015.00041
dc.language.none.fl_str_mv Catalán
cat
language_invalid_str_mv Catalán
language cat
dc.relation.none.fl_str_mv European Commission https://doi.org/10.13039/501100000780 223101
Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2014/SGR-1280
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/3.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/3.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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