Multiomic-based immune response profiling in migraine, vestibular migraine and Meniere's disease

Migraine (MI) is the most common neurological disease, affecting with 20% of the world population. A subset of 25% of MI patients showcase concurrent vestibular symptoms, which may classify as vestibular migraine (VM). Meniere's disease (MD) is a complex inner ear disorder defined by episodes o...

Descripción completa

Detalles Bibliográficos
Autores: Cruz-Granados, Pablo, Frejo, Lidia, Perez-Carpena, Patricia, Lopez-Escamez, Jose A, Amor-Dorado, Juan Carlos, Dominguez-Duran, Emilio, Fernandez-Nava, Maria Jose, Batuecas-Caletrio, Angel, Haro-Hernandez, Elisheba, Martinez-Martinez, Marta
Tipo de recurso: artículo
Fecha de publicación:2024
País:España
Institución:Conselleria de Salut i Consum del Govern de les Illes Balears
Repositorio:Docusalut
Idioma:inglés
OAI Identifier:oai:docusalut.com:20.500.13003/21106
Acceso en línea:https://hdl.handle.net/20.500.13003/21106
Access Level:acceso abierto
Palabra clave:Adult
Aged
Case-Control Studies
Female
Gene Expression Profiling
Humans
Killer Cells, Natural / immunology
Male
Meniere Disease* / genetics
Meniere Disease* / immunology
Middle Aged
Migraine Disorders* / blood
Migraine Disorders* / genetics
Migraine Disorders* / immunology
Transcriptome*
Adulto
Anciano
Estudios de Casos y Controles
Femenino
Perfilación de la Expresión Génica
Humanos
Masculino
Persona de Mediana Edad
Transcriptoma
Descripción
Sumario:Migraine (MI) is the most common neurological disease, affecting with 20% of the world population. A subset of 25% of MI patients showcase concurrent vestibular symptoms, which may classify as vestibular migraine (VM). Meniere's disease (MD) is a complex inner ear disorder defined by episodes of vertigo associated with tinnitus and sensorineural hearing loss with a significant autoimmune/autoinflammatory contribution, which symptoms overlap with VM. Blood samples from 18 patients with MI (5), VM (5) and MD (8) and 6 controls were collected and compared in a case-control study. Droplet-isolated nuclei from mononuclear cells used to generate scRNAseq and scATACseq data sets from MI, VM and MD. MI and VM have no differences in their immune transcriptome; therefore, they were considered as a single cluster for further analyses. Natural Killer (NK) cells transcriptomic data support a polarisation triggered by Type 1 innate immune cells via the release of interleukin (IL)-12, IL-15 and IL-18. According to the monocyte scRNAseq data, there were two MD clusters, one inactive and one driven by monocytes. The unique pathways of the MI + VM cluster were cellular responses to metal ions, whereas MD monocyte-driven cluster pathways showed responses to biotic stimuli. MI and MD have different immune responses. These findings support that MI and VM have a Type 1 immune lymphoid cell response, and that there are two clusters of MD patients, one inactive and one Monocyte-driven.