Implementation of anti-HDV reflex testing among HBsAg-positive individuals increases testing for hepatitis D

Although EASL guidelines recommend anti-HDV testing in all HBsAg-positive individuals, HDV infection remains an underdiagnosed condition. We describe the impact of an HDV screening program by reflex anti-HDV testing in all HBsAg-positive samples and compare the results before and after its implement...

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Detalles Bibliográficos
Autores: Palom, Adriana|||0000-0002-0130-1302, Rando-Segura, Ariadna|||0000-0003-4555-7286, Vico, Judit, Pacín Ruiz, Beatriz|||0000-0001-8718-6589, Vargas, Elena, Barreira-Diaz, Ana|||0000-0002-4368-8939, Rodríguez Frías, Francisco|||0000-0002-9128-7013, Riveiro Barciela, Mar|||0000-0001-9309-2052, Esteban, Rafael|||0000-0001-5280-392X, Buti, Maria|||0000-0002-0732-3078
Tipo de recurso: artículo
Fecha de publicación:2022
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:292192
Acceso en línea:https://ddd.uab.cat/record/292192
https://dx.doi.org/urn:doi:10.1016/j.jhepr.2022.100547
Access Level:acceso abierto
Palabra clave:AASLD, American Association for the Study of Liver Diseases
ALT, alanine aminotransferase
APASL, Asian-Pacific Association for the Study of the Liver
APRI, AST to platelet ratio index
AST, aspartate aminotransferase
Anti-HDV reflex testing
Anti-HDV screening
Chronic hepatitis D
FIB-4, fibrosis-4
GGT, gamma glutamyl transferase
HDV diagnosis
PWID, people who inject drugs
Descripción
Sumario:Although EASL guidelines recommend anti-HDV testing in all HBsAg-positive individuals, HDV infection remains an underdiagnosed condition. We describe the impact of an HDV screening program by reflex anti-HDV testing in all HBsAg-positive samples and compare the results before and after its implementation. In total, 2,236 HBsAg-positive determinations were included from January 2018 to December 2021. Only the first sample from each participant was evaluated: 1,492 samples before reflex anti-HDV testing (2018-2020) and 744 samples after (2021). Demographic and clinical characteristics of anti-HDV-positive patients were collected. Before reflex testing, anti-HDV had been tested in 7.6% (114/1492) of HBsAg-positive individuals: 23% (91/390) attended in an academic hospital and only 2% (23/1,102) in primary care centres. After reflex testing was established, 93% (691/744) of HBsAg-positive cases were evaluated for anti-HDV: 91% (533/586) in the academic hospital and 100% (158/158) in primary care. The anti-HDV-positive prevalence was similar before and after reflex testing: 9.6% (11/114) and 8.1% (56/691), respectively. However, the absolute number of anti-HDV-positive patients increased. Most anti-HDV-positive patients were young, HBeAg-negative, Caucasian males. HDV-RNA was detectable in 35 (65%) of 54 tested, HBV-DNA was undetectable in 64%, and alanine aminotransferase levels were normal in 48%. Anti-HDV reflex testing quintupled the absolute number of diagnoses of chronic hepatitis D infection. Before the reflex test, a large percentage of HBsAg-positive individuals had not undergone any anti-HDV determination. Implementation of reflex testing increases the diagnosis of patients with chronic hepatitis D. Chronic hepatitis delta (CHD) is a viral disease caused by HDV, which requires the presence of HBV to propagate. HDV infection can cause rapid progression to cirrhosis, among other severe complications. The prevalence of CHD worldwide is controversial, and the infection often goes unrecognised, mainly because of unawareness among physicians. Use of reflex testing in other viral hepatitis has proven to increase detection and linking-to-care of infected patients. Implementation of anti-HDV testing in all HBsAg-positive patients has led to a 5-fold increase in the number of HDV diagnoses in an academic hospital and primary care centres.