Polyphasic Taxonomy of a Novel Yeast Isolated from Antarctic environment; Description of <i>Cryptococcus victoriae</i> sp. nov

In 1992 some samples of mosses, lichens and soils were collected from Botany Bay, Southern Victoria Land (77° 01′ S 162° 32′ E) and, as a result of a routine screening programme some yeasts were isolated. One of them, designated as strain G5, showed marked differences when compared to other antarcti...

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Autores: Montes López, María Jesús, Belloch, Carmela, Galiana, Mónica, García, María Dolores, Andrés Lacueva, Ma. Cristina, Ferrer, Santiago, Torres-Rodriguez, Jose María, Guinea, Jesús
Tipo de documento: artigo
Estado:Versión aceptada para publicación
Data de publicação:1999
País:España
Recursos:Universidad de Barcelona
Repositório:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/218305
Acesso em linha:https://hdl.handle.net/2445/218305
Access Level:Acceso aberto
Palavra-chave:Bacteris
Taxonomia (Biologia)
Antàrtida
Bacteria
Taxonomy (Biology)
Antarctica
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spelling Polyphasic Taxonomy of a Novel Yeast Isolated from Antarctic environment; Description of <i>Cryptococcus victoriae</i> sp. novMontes López, María JesúsBelloch, CarmelaGaliana, MónicaGarcía, María DoloresAndrés Lacueva, Ma. CristinaFerrer, SantiagoTorres-Rodriguez, Jose MaríaGuinea, JesúsBacterisTaxonomia (Biologia)AntàrtidaBacteriaTaxonomy (Biology)AntarcticaIn 1992 some samples of mosses, lichens and soils were collected from Botany Bay, Southern Victoria Land (77° 01′ S 162° 32′ E) and, as a result of a routine screening programme some yeasts were isolated. One of them, designated as strain G5, showed marked differences when compared to other antarctic yeasts. According to morphological and physiological characteristics, we were able to identify the strain G5 as a yeast belonging to the genus Cryptococcus. Some characteristics of this genus are the growth response to myo-inositol, celobiose, raffinose and D-glucuronate, no-fermentation, the absence of mycelium and pseudomycelium, asexual reproduction, Diazolium blue B test (DBB) and urea hydrolisis positive and the growth without vitamines. This strain (G5) formed cream colonies of slimy appearance with cells of 3×2 urn in size, that grew between 4 °C and 20 °C. The G+C content of strain G5 was 50.3 mol%. The molecular characterization by whole-cell proteins and RFLP analysis of the 5.8S rRNA gene and the two ribosomal internal transcribed spacers (5.8S-ITS region), revealed that this strain was different from other antarctic species of this genus. The phylogenetic tree deduced from the 5.8S rRNA gene sequence showed the strain G5 as a member of the genus Cryptococcus, clearly separated from other basidiomycetous yeasts. On the basis of the physiological, genotypical and phylogenetical data, the new isolate G5 was described as Cryptococcus victoriae, sp. nov., with the type strain G5 (= CECT 11114).Elsevier1999info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttps://hdl.handle.net/2445/218305Articles publicats en revistes (Biologia, Sanitat i Medi Ambient)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésVersió postprint del document publicat a:Systematic and Applied Microbiology, 1999, vol. 22, p. 97-105(c) Elsevier, 1999info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/2183052026-05-27T06:46:51Z
dc.title.none.fl_str_mv Polyphasic Taxonomy of a Novel Yeast Isolated from Antarctic environment; Description of <i>Cryptococcus victoriae</i> sp. nov
title Polyphasic Taxonomy of a Novel Yeast Isolated from Antarctic environment; Description of <i>Cryptococcus victoriae</i> sp. nov
spellingShingle Polyphasic Taxonomy of a Novel Yeast Isolated from Antarctic environment; Description of <i>Cryptococcus victoriae</i> sp. nov
Montes López, María Jesús
Bacteris
Taxonomia (Biologia)
Antàrtida
Bacteria
Taxonomy (Biology)
Antarctica
title_short Polyphasic Taxonomy of a Novel Yeast Isolated from Antarctic environment; Description of <i>Cryptococcus victoriae</i> sp. nov
title_full Polyphasic Taxonomy of a Novel Yeast Isolated from Antarctic environment; Description of <i>Cryptococcus victoriae</i> sp. nov
title_fullStr Polyphasic Taxonomy of a Novel Yeast Isolated from Antarctic environment; Description of <i>Cryptococcus victoriae</i> sp. nov
title_full_unstemmed Polyphasic Taxonomy of a Novel Yeast Isolated from Antarctic environment; Description of <i>Cryptococcus victoriae</i> sp. nov
title_sort Polyphasic Taxonomy of a Novel Yeast Isolated from Antarctic environment; Description of <i>Cryptococcus victoriae</i> sp. nov
dc.creator.none.fl_str_mv Montes López, María Jesús
Belloch, Carmela
Galiana, Mónica
García, María Dolores
Andrés Lacueva, Ma. Cristina
Ferrer, Santiago
Torres-Rodriguez, Jose María
Guinea, Jesús
author Montes López, María Jesús
author_facet Montes López, María Jesús
Belloch, Carmela
Galiana, Mónica
García, María Dolores
Andrés Lacueva, Ma. Cristina
Ferrer, Santiago
Torres-Rodriguez, Jose María
Guinea, Jesús
author_role author
author2 Belloch, Carmela
Galiana, Mónica
García, María Dolores
Andrés Lacueva, Ma. Cristina
Ferrer, Santiago
Torres-Rodriguez, Jose María
Guinea, Jesús
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Bacteris
Taxonomia (Biologia)
Antàrtida
Bacteria
Taxonomy (Biology)
Antarctica
topic Bacteris
Taxonomia (Biologia)
Antàrtida
Bacteria
Taxonomy (Biology)
Antarctica
description In 1992 some samples of mosses, lichens and soils were collected from Botany Bay, Southern Victoria Land (77° 01′ S 162° 32′ E) and, as a result of a routine screening programme some yeasts were isolated. One of them, designated as strain G5, showed marked differences when compared to other antarctic yeasts. According to morphological and physiological characteristics, we were able to identify the strain G5 as a yeast belonging to the genus Cryptococcus. Some characteristics of this genus are the growth response to myo-inositol, celobiose, raffinose and D-glucuronate, no-fermentation, the absence of mycelium and pseudomycelium, asexual reproduction, Diazolium blue B test (DBB) and urea hydrolisis positive and the growth without vitamines. This strain (G5) formed cream colonies of slimy appearance with cells of 3×2 urn in size, that grew between 4 °C and 20 °C. The G+C content of strain G5 was 50.3 mol%. The molecular characterization by whole-cell proteins and RFLP analysis of the 5.8S rRNA gene and the two ribosomal internal transcribed spacers (5.8S-ITS region), revealed that this strain was different from other antarctic species of this genus. The phylogenetic tree deduced from the 5.8S rRNA gene sequence showed the strain G5 as a member of the genus Cryptococcus, clearly separated from other basidiomycetous yeasts. On the basis of the physiological, genotypical and phylogenetical data, the new isolate G5 was described as Cryptococcus victoriae, sp. nov., with the type strain G5 (= CECT 11114).
publishDate 1999
dc.date.none.fl_str_mv 1999
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/218305
url https://hdl.handle.net/2445/218305
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Versió postprint del document publicat a:
Systematic and Applied Microbiology, 1999, vol. 22, p. 97-105
dc.rights.none.fl_str_mv (c) Elsevier, 1999
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) Elsevier, 1999
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Articles publicats en revistes (Biologia, Sanitat i Medi Ambient)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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