Haplosporidium pinnae Parasite Detection in Seawater Samples

In this study, we investigated the presence of the parasite Haplosporidium pinnae, which is a pathogen for the bivalve Pinna nobilis, in water samples from different environments. Fifteen mantle samples of P. nobilis infected by H. pinnae were used to characterize the ribosomal unit of this parasite...

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Detalhes bibliográficos
Autores: Moro-Martínez, Irene, Vázquez-Luis, Maite, García-March, José Rafael, Prado, Patricia, Mičić, Milena, Catanese, Gaetano
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/337978
Acesso em linha:http://hdl.handle.net/10261/337978
Access Level:acceso abierto
Palavra-chave:Haplosporidium pinnae
eDNA
ribosomal unit
Pinna nobilis
benthic invertebrate
critically endangered
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spelling Haplosporidium pinnae Parasite Detection in Seawater SamplesMoro-Martínez, IreneVázquez-Luis, MaiteGarcía-March, José RafaelPrado, PatriciaMičić, MilenaCatanese, GaetanoHaplosporidium pinnaeeDNAribosomal unitPinna nobilisbenthic invertebratecritically endangeredIn this study, we investigated the presence of the parasite Haplosporidium pinnae, which is a pathogen for the bivalve Pinna nobilis, in water samples from different environments. Fifteen mantle samples of P. nobilis infected by H. pinnae were used to characterize the ribosomal unit of this parasite. The obtained sequences were employed to develop a method for eDNA detection of H. pinnae. We collected 56 water samples (from aquaria, open sea and sanctuaries) for testing the methodology. In this work, we developed three different PCRs generating amplicons of different lengths to determine the level of degradation of the DNA, since the status of H. pinnae in water and, therefore, its infectious capacity are unknown. The results showed the ability of the method to detect H. pinnae in sea waters from different areas persistent in the environment but with different degrees of DNA fragmentation. This developed method offers a new tool for preventive analysis for monitoring areas and to better understand the life cycle and the spread of this parasite.This research was partially funded by EsMarEs (Ministerio para la Transición Ecológica, Spanish Government), and the European Union’s LIFE programme through the projects LIFE INTEMARES (LIFE15 IPE ES 012, coordinated by the Biodiversity Foundation of the Ministry for the Ecological Transition and the Demographic Challenge) and LIFE PINNARCA (NAT/ES/001265).Peer reviewedMultidisciplinary Digital Publishing InstituteMinisterio para la Transición Ecológica y el Reto Demográfico (España)Fundación BiodiversidadConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2023202320232023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/337978reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.3390/microorganisms11051146Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3379782026-05-22T06:33:51Z
dc.title.none.fl_str_mv Haplosporidium pinnae Parasite Detection in Seawater Samples
title Haplosporidium pinnae Parasite Detection in Seawater Samples
spellingShingle Haplosporidium pinnae Parasite Detection in Seawater Samples
Moro-Martínez, Irene
Haplosporidium pinnae
eDNA
ribosomal unit
Pinna nobilis
benthic invertebrate
critically endangered
title_short Haplosporidium pinnae Parasite Detection in Seawater Samples
title_full Haplosporidium pinnae Parasite Detection in Seawater Samples
title_fullStr Haplosporidium pinnae Parasite Detection in Seawater Samples
title_full_unstemmed Haplosporidium pinnae Parasite Detection in Seawater Samples
title_sort Haplosporidium pinnae Parasite Detection in Seawater Samples
dc.creator.none.fl_str_mv Moro-Martínez, Irene
Vázquez-Luis, Maite
García-March, José Rafael
Prado, Patricia
Mičić, Milena
Catanese, Gaetano
author Moro-Martínez, Irene
author_facet Moro-Martínez, Irene
Vázquez-Luis, Maite
García-March, José Rafael
Prado, Patricia
Mičić, Milena
Catanese, Gaetano
author_role author
author2 Vázquez-Luis, Maite
García-March, José Rafael
Prado, Patricia
Mičić, Milena
Catanese, Gaetano
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio para la Transición Ecológica y el Reto Demográfico (España)
Fundación Biodiversidad
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Haplosporidium pinnae
eDNA
ribosomal unit
Pinna nobilis
benthic invertebrate
critically endangered
topic Haplosporidium pinnae
eDNA
ribosomal unit
Pinna nobilis
benthic invertebrate
critically endangered
description In this study, we investigated the presence of the parasite Haplosporidium pinnae, which is a pathogen for the bivalve Pinna nobilis, in water samples from different environments. Fifteen mantle samples of P. nobilis infected by H. pinnae were used to characterize the ribosomal unit of this parasite. The obtained sequences were employed to develop a method for eDNA detection of H. pinnae. We collected 56 water samples (from aquaria, open sea and sanctuaries) for testing the methodology. In this work, we developed three different PCRs generating amplicons of different lengths to determine the level of degradation of the DNA, since the status of H. pinnae in water and, therefore, its infectious capacity are unknown. The results showed the ability of the method to detect H. pinnae in sea waters from different areas persistent in the environment but with different degrees of DNA fragmentation. This developed method offers a new tool for preventive analysis for monitoring areas and to better understand the life cycle and the spread of this parasite.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/337978
url http://hdl.handle.net/10261/337978
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.3390/microorganisms11051146
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,198674

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