Carotenoid deficiency triggers autophagy in the model green alga Chlamydomonas reinhardtii

All aerobic organisms have developed sophisticated mechanisms to prevent, detect and respond to cell damage caused by the unavoidable production of reactive oxygen species (ROS). Plants and algae are able to synthesize specific pigments in the chloroplast called carotenoids to prevent photo-oxidativ...

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Detalhes bibliográficos
Autores: Pérez Pérez, María Esther, Couso, Inmaculada, Crespo, José L.
Tipo de documento: artigo
Estado:Versión aceptada para publicación
Data de publicação:2012
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositório:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/377515
Acesso em linha:http://hdl.handle.net/10261/377515
https://api.elsevier.com/content/abstract/scopus_id/84859126220
Access Level:Acceso aberto
Palavra-chave:Carotenoid | Chlamydomonas | High light stress | Oxidative stress | Plant | ROS
Chlamydomonas
High light stress
Oxidative stress
Plant
ROS
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spelling Carotenoid deficiency triggers autophagy in the model green alga Chlamydomonas reinhardtiiPérez Pérez, María EstherCouso, InmaculadaCrespo, José L.Carotenoid | Chlamydomonas | High light stress | Oxidative stress | Plant | ROSChlamydomonasHigh light stressOxidative stressPlantROSAll aerobic organisms have developed sophisticated mechanisms to prevent, detect and respond to cell damage caused by the unavoidable production of reactive oxygen species (ROS). Plants and algae are able to synthesize specific pigments in the chloroplast called carotenoids to prevent photo-oxidative damage caused by highly reactive by-products of photosynthesis. In this study we used the unicellular green alga Chlamydomonas reinhardtii to demonstrate that defects in carotenoid biosynthesis lead to the activation of autophagy, a membrane-trafficking process that participates in the recycling and degradation of damaged or toxic cellular components. Carotenoid depletion caused by either the mutation of phytoene synthase or the inhibition of phytoene desaturase by the herbicide norflurazon, resulted in a strong induction of autophagy. We found that high light transiently activates autophagy in wild-type Chlamydomonas cells as part of an adaptation response to this stress. Our results showed that a Chlamydomonas mutant defective in the synthesis of specific carotenoids that accumulate during high light stress exhibits constitutive autophagy. Moreover, inhibition of the ROS-generating NADPH oxidase partially reduced the autophagy induction associated to carotenoid deficiency, which revealed a link between photo-oxidative damage, ROS accumulation and autophagy activation in Chlamydomonas cells with a reduced carotenoid content.We thank the Chlamydomonas Center culture collection for providing Chlamydomonas strains and in particular to Krishna Niyogi and Arthur Grossman for making carotenoid mutant strains available to the research community. This work was supported by the Spanish Ministry of Science and Innovation (grant BFU2009-07368 to J.L.C.).Peer reviewedTaylor & FrancisMinisterio de Ciencia e Innovación (España)Pérez Pérez, María Esther [0000-0003-0779-6665]Couso, Inmaculada [0000-0003-2849-675X]Crespo, José L [0000-0003-3514-1025]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202520252012info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionaplication/pdfhttp://hdl.handle.net/10261/377515https://api.elsevier.com/content/abstract/scopus_id/84859126220reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.4161/auto.18864Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3775152026-05-22T06:33:51Z
dc.title.none.fl_str_mv Carotenoid deficiency triggers autophagy in the model green alga Chlamydomonas reinhardtii
title Carotenoid deficiency triggers autophagy in the model green alga Chlamydomonas reinhardtii
spellingShingle Carotenoid deficiency triggers autophagy in the model green alga Chlamydomonas reinhardtii
Pérez Pérez, María Esther
Carotenoid | Chlamydomonas | High light stress | Oxidative stress | Plant | ROS
Chlamydomonas
High light stress
Oxidative stress
Plant
ROS
title_short Carotenoid deficiency triggers autophagy in the model green alga Chlamydomonas reinhardtii
title_full Carotenoid deficiency triggers autophagy in the model green alga Chlamydomonas reinhardtii
title_fullStr Carotenoid deficiency triggers autophagy in the model green alga Chlamydomonas reinhardtii
title_full_unstemmed Carotenoid deficiency triggers autophagy in the model green alga Chlamydomonas reinhardtii
title_sort Carotenoid deficiency triggers autophagy in the model green alga Chlamydomonas reinhardtii
dc.creator.none.fl_str_mv Pérez Pérez, María Esther
Couso, Inmaculada
Crespo, José L.
author Pérez Pérez, María Esther
author_facet Pérez Pérez, María Esther
Couso, Inmaculada
Crespo, José L.
author_role author
author2 Couso, Inmaculada
Crespo, José L.
author2_role author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
Pérez Pérez, María Esther [0000-0003-0779-6665]
Couso, Inmaculada [0000-0003-2849-675X]
Crespo, José L [0000-0003-3514-1025]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Carotenoid | Chlamydomonas | High light stress | Oxidative stress | Plant | ROS
Chlamydomonas
High light stress
Oxidative stress
Plant
ROS
topic Carotenoid | Chlamydomonas | High light stress | Oxidative stress | Plant | ROS
Chlamydomonas
High light stress
Oxidative stress
Plant
ROS
description All aerobic organisms have developed sophisticated mechanisms to prevent, detect and respond to cell damage caused by the unavoidable production of reactive oxygen species (ROS). Plants and algae are able to synthesize specific pigments in the chloroplast called carotenoids to prevent photo-oxidative damage caused by highly reactive by-products of photosynthesis. In this study we used the unicellular green alga Chlamydomonas reinhardtii to demonstrate that defects in carotenoid biosynthesis lead to the activation of autophagy, a membrane-trafficking process that participates in the recycling and degradation of damaged or toxic cellular components. Carotenoid depletion caused by either the mutation of phytoene synthase or the inhibition of phytoene desaturase by the herbicide norflurazon, resulted in a strong induction of autophagy. We found that high light transiently activates autophagy in wild-type Chlamydomonas cells as part of an adaptation response to this stress. Our results showed that a Chlamydomonas mutant defective in the synthesis of specific carotenoids that accumulate during high light stress exhibits constitutive autophagy. Moreover, inhibition of the ROS-generating NADPH oxidase partially reduced the autophagy induction associated to carotenoid deficiency, which revealed a link between photo-oxidative damage, ROS accumulation and autophagy activation in Chlamydomonas cells with a reduced carotenoid content.
publishDate 2012
dc.date.none.fl_str_mv 2012
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/377515
https://api.elsevier.com/content/abstract/scopus_id/84859126220
url http://hdl.handle.net/10261/377515
https://api.elsevier.com/content/abstract/scopus_id/84859126220
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.4161/auto.18864
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv aplication/pdf
dc.publisher.none.fl_str_mv Taylor & Francis
publisher.none.fl_str_mv Taylor & Francis
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812429

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